B0R5Y8 (GCST_HALS3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable aminomethyltransferase
EC=2.1.2.10

Alternative name(s):
Glycine cleavage system T protein

Gene names

Name:	gcvT
Ordered Locus Names:	OE3278R

Organism

Halobacterium salinarum (strain ATCC 29341 / DSM 671 / R1) [Complete proteome] [HAMAP]

Taxonomic identifier

478009 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Halobacteria › Halobacteriales › Halobacteriaceae › Halobacterium

Protein attributes

Sequence length	363 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The glycine cleavage system catalyzes the degradation of glycine By similarity. HAMAP MF_00259
Catalytic activity	[Protein]-S(8)-aminomethyldihydrolipoyllysine + tetrahydrofolate = [protein]-dihydrolipoyllysine + 5,10-methylenetetrahydrofolate + NH3. HAMAP MF_00259
Subunit structure	The glycine cleavage system is composed of four proteins: P, T, L and H By similarity.
Sequence similarities	Belongs to the GcvT family.

Ontologies

Keywords
Molecular function	Aminotransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycine catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	aminomethyltransferase activity Inferred from electronic annotation. Source: EC transaminase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 363	363	Probable aminomethyltransferase HAMAP MF_00259		PRO_1000114098

Sequences

	Sequence		Length	Mass (Da)
	B0R5Y8 [UniParc]. Last modified April 8, 2008. Version 1. Checksum: FD25399E1A94FE70	FASTA	363	38,199
	10 20 30 40 50 60 MGLRTSPLHG RHEDRGASFT EFGGWNMPVD FDGIQAEHAA VREAAGIFDV SHMGEIEVSG 70 80 90 100 110 120 PDAERLMQRL TTNDVSRLDP GDAQYAAITD DDGIMIDDTV VYRTPADWPG AFLFVPNAGH 130 140 150 160 170 180 DAAAFDRWTD HRDAHDLDAS VDNVTTDYGM VAVQGPDAPD LVAARAGDGV HDLGRFEAAT 190 200 210 220 230 240 VGVAGVECLV ANTGYTGEAG VEIVFPADGA GAVWDAIAND CQPCGLGARD TLRMEHGFLL 250 260 270 280 290 300 SGQDFDPEEN PRTPFEAGIG FAVAPESGFV GRDALADTDS PEQQFVGLTL DERGVPRHGY 310 320 330 340 350 360 AVTTPAGDEI GTVTSGTMSP TLGEPIGLGY VDSAHAADGT TVAVRIRGTD KQATITTPPF LDQ

References

[1]

"Evolution in the laboratory: the genome of Halobacterium salinarum strain R1 compared to that of strain NRC-1." Pfeiffer F., Schuster S.C., Broicher A., Falb M., Palm P., Rodewald K., Ruepp A., Soppa J., Tittor J., Oesterhelt D. Genomics 91:335-346(2008) [PubMed: 18313895] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29341 / DSM 671 / R1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM774415 Genomic DNA. Translation: CAP14157.1.
RefSeq	YP_001689503.1. NC_010364.1.
3D structure databases
ProteinModelPortal	B0R5Y8.
ModBase	Search...
Protein-protein interaction databases
STRING	B0R5Y8.
Proteomic databases
PRIDE	B0R5Y8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5953781.
GenomeReviews	Gene locus OE3278R in contig AM774415_GR.
KEGG	hsl:OE3278R.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG299834.
OMA	DAIANDC.
PhylomeDB	B0R5Y8.
ProtClustDB	PRK00389.
Enzyme and pathway databases
BioCyc	HSAL478009:OE3278R-MONOMER.
Family and domain databases
HAMAP	MF_00259. GcvT. [Tree]
InterPro	IPR013977. GCV_T_C. IPR006222. GCV_T_N. IPR006223. GcvT. IPR022903. NH2_Me_Trfase. [Graphical view]
KO	K00605.
PANTHER	PTHR13847:SF5. PTHR13847:SF5. 1 hit.
Pfam	PF01571. GCV_T. 1 hit. PF08669. GCV_T_C. 1 hit. [Graphical view]
PIRSF	PIRSF006487. GcvT. 1 hit.
TIGRFAMs	TIGR00528. GcvT. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GCST_HALS3

Accession

Primary (citable) accession number: B0R5Y8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	April 8, 2008
Last modified:	December 14, 2011
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program