B0R5S5 (KITH_HALS3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thymidine kinase
EC=2.7.1.21

Gene names

Name:	tdk
Ordered Locus Names:	OE_3159R

Organism

Halobacterium salinarum (strain ATCC 29341 / DSM 671 / R1) [Complete proteome] [HAMAP]

Taxonomic identifier

478009 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Halobacteria › Halobacteriales › Halobacteriaceae › Halobacterium › Halobacterium salinarum

Protein attributes

Sequence length	195 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + thymidine = ADP + thymidine 5'-phosphate. HAMAP-Rule MF_00124
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm Potential HAMAP-Rule MF_00124.
Sequence similarities	Belongs to the thymidine kinase family.

Ontologies

Keywords
Biological process	DNA synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP thymidine kinase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 195	195	Thymidine kinase HAMAP-Rule MF_00124		PRO_1000095433
Regions
	Nucleotide binding	15 – 22	8	ATP By similarity
	Nucleotide binding	91 – 94	4	ATP By similarity
Sites
	Active site	92	1	Proton acceptor Potential
	Metal binding	148	1	Zinc By similarity
	Metal binding	151	1	Zinc By similarity
	Metal binding	186	1	Zinc By similarity
	Metal binding	189	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	B0R5S5 [UniParc]. Last modified April 8, 2008. Version 1. Checksum: EBA3E45144CD3B1E	FASTA	195	21,305
	10 20 30 40 50 60 MHAITNSGWV EVITGSMFSG KTEELLRRLR RAEIAGQDVA AVTPAVDDRY GEATLGSHAG 70 80 90 100 110 120 RSWAATVVEP TAEGVASIPT LLNGEQVVAI DEANFFPAEL VDVCQELAAD GRRVVLSGTD 130 140 150 160 170 180 QTFRGEPFEP VPQLMAIAEY VDKMRAICMQ CGEPATRNQR LIEGEPAHYD DPTVMVGAEE 190 TYEARCRNCH VVRRE

References

[1]

"Evolution in the laboratory: the genome of Halobacterium salinarum strain R1 compared to that of strain NRC-1." Pfeiffer F., Schuster S.C., Broicher A., Falb M., Palm P., Rodewald K., Ruepp A., Soppa J., Tittor J., Oesterhelt D. Genomics 91:335-346(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29341 / DSM 671 / R1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM774415 Genomic DNA. Translation: CAP14092.1.
RefSeq	YP_001689440.1. NC_010364.1.
3D structure databases
ProteinModelPortal	B0R5S5.
ModBase	Search...
Protein-protein interaction databases
STRING	478009.OE3159R.
Proteomic databases
PRIDE	B0R5S5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAP14092; CAP14092; OE_3159R.
GeneID	5953011.
KEGG	hsl:OE3159R.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1435.
HOGENOM	HOG000076390.
KO	K00857.
OMA	KVHAICV.
ProtClustDB	PRK04296.
Enzyme and pathway databases
BioCyc	HSAL478009:GJVF-1240-MONOMER.
Family and domain databases
HAMAP	MF_00124. Thymidine_kinase.
InterPro	IPR001267. Thymidine_kinase. IPR020633. Thymidine_kinase_CS. IPR020634. Thymidine_kinase_subgr. [Graphical view]
PANTHER	PTHR11441. PTHR11441. 1 hit.
Pfam	PF00265. TK. 1 hit. [Graphical view]
PIRSF	PIRSF035805. TK_cell. 1 hit.
PROSITE	PS00603. TK_CELLULAR_TYPE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KITH_HALS3

Accession

Primary (citable) accession number: B0R5S5

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	April 8, 2008
Last modified:	May 1, 2013
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program