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B0R5R0 (B0R5R0_HALS3) Unreviewed, UniProtKB/TrEMBL

Last modified January 25, 2012. Version 25. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Amidophosphoribosyltransferase PIRNR PIRNR000485
Short name=ATase PIRNR PIRNR000485
EC=2.4.2.14 PIRNR PIRNR000485
Alternative name(s):
Glutamine phosphoribosylpyrophosphate amidotransferase PIRNR PIRNR000485
Gene names
Name:purF EMBL CAP14077.1
Ordered Locus Names:OE3139R
OrganismHalobacterium salinarum (strain ATCC 29341 / DSM 671 / R1) [Complete proteome] [HAMAP]
Taxonomic identifier478009 [NCBI]
Taxonomic lineageArchaea › Euryarchaeota › Halobacteria › Halobacteriales › Halobacteriaceae › Halobacterium

Protein attributes

Sequence length490 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O. PIRNR PIRNR000485

Cofactor

Binds 1 4Fe-4S cluster per subunit By similarity. PIRSR PIRSR000485-3

Binds 1 magnesium ion per subunit By similarity. PIRNR PIRNR000485

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/2. PIRNR PIRNR000485

Sequence similarities

In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family. PIRNR PIRNR000485

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Active site181For GATase activity By similarity PIRSR PIRSR000485-1
Metal binding2561Iron-sulfur (4Fe-4S) By similarity PIRSR PIRSR000485-3
Metal binding4071Iron-sulfur (4Fe-4S) By similarity PIRSR PIRSR000485-3
Metal binding4581Iron-sulfur (4Fe-4S) By similarity PIRSR PIRSR000485-3
Metal binding4611Iron-sulfur (4Fe-4S) By similarity PIRSR PIRSR000485-3

Sequences

Sequence LengthMass (Da)Tools
B0R5R0 [UniParc].

Last modified April 8, 2008. Version 1.
Checksum: DC8D768185FF98EF

FASTA49052,028
        10         20         30         40         50         60 
MSGGPADGPM SDHPTEKCGV VGASLSARDA ALPTYYALYA LQHRGQESAG IVAHDGFQQH 

        70         80         90        100        110        120 
QHVGMGLVGD AFDEADIDAL HGSAAIGHVR YPTAGSVDKS CAQPFSVSFK GGALGLSHNG 

       130        140        150        160        170        180 
NLVNADELRA DLAASGHAFT SDGDTEVIAH DLARNLLDAD LVDAVERTME RIHGSYSLTI 

       190        200        210        220        230        240 
MHDDTVLGVR DPRGNRPLVL GTVEDGYVIA SESAAIDTIG GDVVRDVRPG EAIVLDDDGT 

       250        260        270        280        290        300 
GFSAHQLVDR DTTAHCFFEY VYFARPDSVI DDELVYEVRR NLGRQLWHET GVESDVVVPV 

       310        320        330        340        350        360 
PDSGRAFASG YADAATEDGS DVEFAEGLMK NRYVGRTFIM PTQEARERAV RLKLNPIKST 

       370        380        390        400        410        420 
VEGQSVTIID DSIVRGTTSS QLVDLVRDAG ATEVHVRIGA PQIQAPCYFG IDMATREELI 

       430        440        450        460        470        480 
AADRTADEIA AEIGADSLSY LPLSAVTAAI GKSEADMCAG CVTGAYPYDV DGEATDRDAE 

       490 
RPTVTPSSDD

« Hide

References

[1]"Evolution in the laboratory: the genome of Halobacterium salinarum strain R1 compared to that of strain NRC-1."
Pfeiffer F., Schuster S.C., Broicher A., Falb M., Palm P., Rodewald K., Ruepp A., Soppa J., Tittor J., Oesterhelt D.
Genomics 91:335-346(2008) [PubMed: 18313895] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 29341 / DSM 671 / R1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AM774415 Genomic DNA. Translation: CAP14077.1.
RefSeqYP_001689425.1. NC_010364.1.

3D structure databases

ProteinModelPortalB0R5R0.
ModBaseSearch...

Protein-protein interaction databases

STRINGB0R5R0.

Proteomic databases

PRIDEB0R5R0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID5953001.
GenomeReviewsGene locus OE3139R in contig AM774415_GR.
KEGGhsl:OE3139R.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG392416.
OMAVWAPGEE.
PhylomeDBB0R5R0.
ProtClustDBCLSK511436.

Family and domain databases

InterProIPR005854. Amd_phspho_trans.
IPR000583. GATase_2.
IPR017932. GATase_II.
IPR000836. PRibTrfase.
[Graphical view]
KOK00764.
PANTHERPTHR11907. Amd_phspho_trans. 1 hit.
PfamPF00310. GATase_2. 1 hit.
PF00156. Pribosyltran. 1 hit.
[Graphical view]
PIRSFPIRSF000485. Amd_phspho_trans. 1 hit.
TIGRFAMsTIGR01134. PurF. 1 hit.
PROSITEPS51278. GATASE_TYPE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameB0R5R0_HALS3
AccessionPrimary (citable) accession number: B0R5R0
Entry history
Integrated into UniProtKB/TrEMBL: April 8, 2008
Last sequence update: April 8, 2008
Last modified: January 25, 2012
This is version 25 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info