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Protein

Imidazolonepropionase

Gene

hutI

Organism
Halobacterium salinarum (strain ATCC 29341 / DSM 671 / R1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

(S)-3-(5-oxo-4,5-dihydro-3H-imidazol-4-yl)propanoate + H2O = N-formimidoyl-L-glutamate + H+.UniRule annotation

Cofactori

Zn2+UniRule annotation, Fe2+UniRule annotationNote: Binds 1 zinc or iron ion per subunit.UniRule annotation

Pathwayi: L-histidine degradation into L-glutamate

This protein is involved in step 3 of the subpathway that synthesizes N-formimidoyl-L-glutamate from L-histidine.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Probable histidine ammonia-lyase (hutH)
  2. Probable urocanate hydratase (hutU)
  3. Imidazolonepropionase (hutI)
This subpathway is part of the pathway L-histidine degradation into L-glutamate, which is itself part of Amino-acid degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N-formimidoyl-L-glutamate from L-histidine, the pathway L-histidine degradation into L-glutamate and in Amino-acid degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi77 – 771Zinc or ironUniRule annotation
Metal bindingi79 – 791Zinc or ironUniRule annotation
Binding sitei86 – 861SubstrateUniRule annotation
Binding sitei99 – 991SubstrateUniRule annotation
Binding sitei149 – 1491SubstrateUniRule annotation
Binding sitei182 – 1821SubstrateUniRule annotation
Metal bindingi244 – 2441Zinc or ironUniRule annotation
Binding sitei247 – 2471SubstrateUniRule annotation
Metal bindingi323 – 3231Zinc or ironUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Histidine metabolism

Keywords - Ligandi

Iron, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciHSAL478009:GJVF-999-MONOMER.
UniPathwayiUPA00379; UER00551.

Protein family/group databases

MEROPSiM38.980.

Names & Taxonomyi

Protein namesi
Recommended name:
ImidazolonepropionaseUniRule annotation (EC:3.5.2.7UniRule annotation)
Alternative name(s):
Imidazolone-5-propionate hydrolaseUniRule annotation
Gene namesi
Name:hutIUniRule annotation
Ordered Locus Names:OE_2738F
OrganismiHalobacterium salinarum (strain ATCC 29341 / DSM 671 / R1)
Taxonomic identifieri478009 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaHalobacteriaHalobacterialesHalobacteriaceaeHalobacterium
Proteomesi
  • UP000001321 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 417417ImidazolonepropionasePRO_1000121543Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliB0R543.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the HutI family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000218460.
KOiK01468.
OMAiDHCTHLT.

Family and domain databases

Gene3Di2.30.40.10. 1 hit.
HAMAPiMF_00372. HutI. 1 hit.
InterProiIPR006680. Amidohydro-rel.
IPR005920. HutI.
IPR011059. Metal-dep_hydrolase_composite.
IPR032466. Metal_Hydrolase.
[Graphical view]
PfamiPF01979. Amidohydro_1. 1 hit.
[Graphical view]
SUPFAMiSSF51338. SSF51338. 2 hits.
SSF51556. SSF51556. 1 hit.
TIGRFAMsiTIGR01224. hutI. 1 hit.

Sequencei

Sequence statusi: Complete.

B0R543-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSLDAVVHG ARELVVGPAA GGDTLETHAD GAVAVVDGAV AAVGDTADVL
60 70 80 90 100
AAYPAENATT AIDATGKTVL PGFVDPHTHA LFAGDRSDEF AAKLRGKPYQ
110 120 130 140 150
EILAEGGGIL RTVDAVRAAS DAALVANLTA QLDVMLAHGT TTAEVKTGYG
160 170 180 190 200
LDTETECRML DAIAAAAAEH PVDVVTTFLG AHAVPDDTDA DAYVDAVIDD
210 220 230 240 250
QLPAAANGPA RFCDVFCEAD VFTVEQSRRI LDAGREHGLA PKLHAEEFTR
260 270 280 290 300
LGGAQLAADL GATSADHLLH ATPEDAAALA DAGVTPVLLP ATAFVLDEAY
310 320 330 340 350
ADPQQFLAAA DNRTGAPVAL GTDLNPNCYT HSMGFVVSLA CNGMRMAPAD
360 370 380 390 400
AVLAATAWAA SALDRGRDGT GTLREGTDGD VLVVDAPSHV HLPYNPGVNN
410
VEAVLTDGTV AVGGGGA
Length:417
Mass (Da):42,297
Last modified:April 8, 2008 - v1
Checksum:i3D2EF2B8918D3CF1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM774415 Genomic DNA. Translation: CAP13858.1.
RefSeqiWP_010902875.1. NC_010364.1.

Genome annotation databases

EnsemblBacteriaiCAP13858; CAP13858; OE_2738F.
GeneIDi5953909.
KEGGihsl:OE_2738F.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM774415 Genomic DNA. Translation: CAP13858.1.
RefSeqiWP_010902875.1. NC_010364.1.

3D structure databases

ProteinModelPortaliB0R543.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiM38.980.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAP13858; CAP13858; OE_2738F.
GeneIDi5953909.
KEGGihsl:OE_2738F.

Phylogenomic databases

HOGENOMiHOG000218460.
KOiK01468.
OMAiDHCTHLT.

Enzyme and pathway databases

UniPathwayiUPA00379; UER00551.
BioCyciHSAL478009:GJVF-999-MONOMER.

Family and domain databases

Gene3Di2.30.40.10. 1 hit.
HAMAPiMF_00372. HutI. 1 hit.
InterProiIPR006680. Amidohydro-rel.
IPR005920. HutI.
IPR011059. Metal-dep_hydrolase_composite.
IPR032466. Metal_Hydrolase.
[Graphical view]
PfamiPF01979. Amidohydro_1. 1 hit.
[Graphical view]
SUPFAMiSSF51338. SSF51338. 2 hits.
SSF51556. SSF51556. 1 hit.
TIGRFAMsiTIGR01224. hutI. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiHUTI_HALS3
AccessioniPrimary (citable) accession number: B0R543
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: April 8, 2008
Last modified: September 7, 2016
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.