B0R542 (HUTG_HALS3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Formimidoylglutamase
EC=3.5.3.8

Alternative name(s):
Formiminoglutamase
Formiminoglutamate hydrolase

Gene names

Name:	hutG
Ordered Locus Names:	OE_2736F

Organism

Halobacterium salinarum (strain ATCC 29341 / DSM 671 / R1) [Complete proteome] [HAMAP]

Taxonomic identifier

478009 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Halobacteria › Halobacteriales › Halobacteriaceae › Halobacterium › Halobacterium salinarum

Protein attributes

Sequence length	306 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	N-formimidoyl-L-glutamate + H2O = L-glutamate + formamide. HAMAP-Rule MF_00737
Cofactor	Binds 2 manganese ions per subunit By similarity.
Pathway	Amino-acid degradation; L-histidine degradation into L-glutamate; L-glutamate from N-formimidoyl-L-glutamate (hydrolase route): step 1/1. HAMAP-Rule MF_00737
Sequence similarities	Belongs to the arginase family.

Ontologies

Keywords
Biological process	Histidine metabolism
Ligand	Manganese Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	histidine catabolic process to glutamate and formamide Inferred from electronic annotation. Source: UniProtKB-UniPathway histidine catabolic process to glutamate and formate Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	formimidoylglutamase activity Inferred from electronic annotation. Source: HAMAP manganese ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 306	306	Formimidoylglutamase HAMAP-Rule MF_00737		PRO_1000133001
Sites
	Metal binding	123	1	Manganese 1 By similarity
	Metal binding	147	1	Manganese 1 By similarity
	Metal binding	147	1	Manganese 2 By similarity
	Metal binding	149	1	Manganese 2 By similarity
	Metal binding	151	1	Manganese 1 By similarity
	Metal binding	234	1	Manganese 1 By similarity
	Metal binding	234	1	Manganese 2 By similarity
	Metal binding	236	1	Manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	B0R542 [UniParc]. Last modified April 8, 2008. Version 1. Checksum: FD98BD7DBEBDF958	FASTA	306	31,422
	10 20 30 40 50 60 MFQNATDWTP TSTDPRDEQF GGVVEPVPTP SDADDYDAVL VGEPYDGAVI GRRGARDGPS 70 80 90 100 110 120 AIRESLAGVK THHFDAGAVS GVADLGDVVL PDGDVADTQA AVREAAAEVH ETAALPVFVG 130 140 150 160 170 180 GDNSLSYANV APLVAADNGA VGVVSVDAHL DCRAVGDRGP TSGTPYRQLF DAGLDALAVV 190 200 210 220 230 240 GARHFETTTT YAGFLRDQGG RIVTSDAVAA DRDGSLDAAR EALDGVDHVY VSVDIDVLDA 250 260 270 280 290 300 AYPGASAPTP GGIQPRELFA LVEALAASDD RIRGFELVET APTLDTGGRT VDAAARTIAH FLAGAQ

References

[1]

"Evolution in the laboratory: the genome of Halobacterium salinarum strain R1 compared to that of strain NRC-1." Pfeiffer F., Schuster S.C., Broicher A., Falb M., Palm P., Rodewald K., Ruepp A., Soppa J., Tittor J., Oesterhelt D. Genomics 91:335-346(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29341 / DSM 671 / R1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM774415 Genomic DNA. Translation: CAP13857.1.
RefSeq	YP_001689206.1. NC_010364.1.
3D structure databases
ProteinModelPortal	B0R542.
ModBase	Search...
Protein-protein interaction databases
STRING	478009.OE2736F.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAP13857; CAP13857; OE_2736F.
GeneID	5953912.
KEGG	hsl:OE2736F.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0010.
HOGENOM	HOG000237658.
KO	K01479.
OMA	KTIRAML.
ProtClustDB	CLSK242790.
Enzyme and pathway databases
BioCyc	HSAL478009:GJVF-998-MONOMER.
UniPathway	UPA00379; UER00552.
Family and domain databases
Gene3D	3.40.800.10. 1 hit.
HAMAP	MF_00737. Formimidoylglutam.
InterPro	IPR005923. HutG. IPR006035. Ureohydrolase. IPR023696. Ureohydrolase_domain. [Graphical view]
PANTHER	PTHR11358. PTHR11358. 1 hit.
Pfam	PF00491. Arginase. 1 hit. [Graphical view]
PIRSF	PIRSF036979. Arginase. 1 hit.
TIGRFAMs	TIGR01227. hutG. 1 hit.
PROSITE	PS01053. ARGINASE_1. False negative. PS51409. ARGINASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HUTG_HALS3

Accession

Primary (citable) accession number: B0R542

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	April 8, 2008
Last modified:	May 1, 2013
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program