B0R4Z6 (SYE_HALS3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS

Gene names

Name:	gltX
Ordered Locus Names:	OE_2652F

Organism

Halobacterium salinarum (strain ATCC 29341 / DSM 671 / R1) [Complete proteome] [HAMAP]

Taxonomic identifier

478009 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Halobacteria › Halobacteriales › Halobacteriaceae › Halobacterium › Halobacterium salinarum

Protein attributes

Sequence length	586 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP-Rule MF_00022
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP-Rule MF_00022
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00022.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: UniProtKB-HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-HAMAP glutamate-tRNA ligase activity Inferred from electronic annotation. Source: UniProtKB-HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 586	586	Glutamate--tRNA ligase HAMAP-Rule MF_00022		PRO_1000090079
Regions
	Motif	114 – 124	11	"HIGH" region HAMAP-Rule MF_00022

Sequences

	Sequence		Length	Mass (Da)
	B0R4Z6 [UniParc]. Last modified April 8, 2008. Version 1. Checksum: E844ED37F8F46776	FASTA	586	65,312
	10 20 30 40 50 60 MDDELRERVR RAAERAALFN ALKHGSDAQV GAIMGPMMGE HPDFRAHGDE IPGVIAPVIE 70 80 90 100 110 120 DVNGMSDDEQ RARLAELAPE DVEELDSEDE GDDHALPALP NAEAYDEVRM RCAPNPNGPW 130 140 150 160 170 180 HVGHARMPSV IGTYKQRYDG SFVVRFDDTD PETKRPDLDA YDQILEDLSY LGFEADEVLT 190 200 210 220 230 240 ASDRVETYYE HARRLIGMGG AYTCSCPGAE FSDLKNSGEA CPHRDKDPET VADEFEAMVD 250 260 270 280 290 300 GEYNSGEMVL RVKTDITHKN PALRDWVAFR MVDTPHPRDA ASGYRCWPML DFQSGVDDHR 310 320 330 340 350 360 TGVTHIIRGI DLQDSAKRQR FLYEYFGWEY PEVVHWGHVQ LDAYDVAMST STIKERIAAG 370 380 390 400 410 420 ELEGWDDPRA PTMQSIRRRG IRGAAVVDAM VELGTSSSDV ELAMSAVYAN NRDLVDDTAP 430 440 450 460 470 480 RQFLVRDGFE ARGEGPEESH EAVEVPVDDG PDEATPQVHP EHPDRGDREI PVGERVLVEA 490 500 510 520 530 540 TDLPAAGDRV WLKGYGPVRY DGERFAFLDA DIDIVREEGV DVVQWVPAEH NVDVRLRTMD 550 560 570 580 GDVTGYAEPG FADRDADEIV QFVRVGFARV DAHRDGGASV AYFTHP

References

[1]

"Evolution in the laboratory: the genome of Halobacterium salinarum strain R1 compared to that of strain NRC-1." Pfeiffer F., Schuster S.C., Broicher A., Falb M., Palm P., Rodewald K., Ruepp A., Soppa J., Tittor J., Oesterhelt D. Genomics 91:335-346(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29341 / DSM 671 / R1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM774415 Genomic DNA. Translation: CAP13811.1.
RefSeq	YP_001689159.1. NC_010364.1.
3D structure databases
ProteinModelPortal	B0R4Z6.
ModBase	Search...
Protein-protein interaction databases
STRING	478009.OE2652F.
Proteomic databases
PRIDE	B0R4Z6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAP13811; CAP13811; OE_2652F.
GeneID	5952846.
KEGG	hsl:OE2652F.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0008.
HOGENOM	HOG000073585.
KO	K01885.
OMA	MRFAPNP.
ProtClustDB	PRK04156.
Enzyme and pathway databases
BioCyc	HSAL478009:GJVF-951-MONOMER.
Family and domain databases
Gene3D	1.10.1160.10. 1 hit. 3.40.50.620. 2 hits.
HAMAP	MF_00022_A. Glu_tRNA_synth_A.
InterPro	IPR004526. Glu-tRNA-synth_arc/euk. IPR000924. Glu/Gln-tRNA-synth. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd. IPR022888. Glu_tRNA_synth_arc. IPR011035. Ribosomal_L25/Gln-tRNA_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
PANTHER	PTHR10119. PTHR10119. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. PF03950. tRNA-synt_1c_C. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF50715. SSF50715. 1 hit.
TIGRFAMs	TIGR00463. gltX_arch. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE_HALS3

Accession

Primary (citable) accession number: B0R4Z6

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	April 8, 2008
Last modified:	October 16, 2013
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program