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Protein

Chemotaxis response regulator protein-glutamate methylesterase/glutamine deamidase

Gene

cheB

Organism
Halobacterium salinarum (strain ATCC 29341 / DSM 671 / R1)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the Htr transducer proteins (methyl-accepting chemotaxis proteins) by CheR. Also required for Htr deamidations, at least at a specific glutamine-glutamate pair in HTR-II and a specific aspartate-glutamine pair in Htr4.1 Publication

Catalytic activityi

Protein L-glutamate O5-methyl ester + H2O = protein L-glutamate + methanol.1 Publication
Protein L-glutamine + H2O = protein L-glutamate + NH3.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei164By similarity1
Active sitei191By similarity1
Active sitei288By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processChemotaxis

Names & Taxonomyi

Protein namesi
Recommended name:
Chemotaxis response regulator protein-glutamate methylesterase/glutamine deamidase (EC:3.1.1.61, EC:3.5.1.44)
Gene namesi
Name:cheB
Ordered Locus Names:OE_2416R
OrganismiHalobacterium salinarum (strain ATCC 29341 / DSM 671 / R1)
Taxonomic identifieri478009 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaHalobacteriaHalobacterialesHalobacteriaceaeHalobacterium
Proteomesi
  • UP000001321 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Deletion leads to the loss of both chemotactic and phototactic responses. Mutants show increased methylation of the Htr transducer proteins.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004290681 – 347Chemotaxis response regulator protein-glutamate methylesterase/glutamine deamidaseAdd BLAST347

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei534-aspartylphosphateBy similarity1

Post-translational modificationi

Phosphorylated by CheA. Phosphorylation suppresses the inhibitory activity of the N-terminal domain (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiB0R4K0.

Structurei

3D structure databases

ProteinModelPortaliB0R4K0.
SMRiB0R4K0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 119Response regulatoryPROSITE-ProRule annotationAdd BLAST117
Domaini152 – 346CheB-type methylesteraseAdd BLAST195

Domaini

The N-terminal regulatory domain inhibits the activity of the C-terminal effector domain.By similarity

Phylogenomic databases

HOGENOMiHOG000151424.
KOiK03412.
OMAiMLEMHRA.
OrthoDBiPOG093Z00HE.

Family and domain databases

CDDicd16432. CheB_Rec. 1 hit.
cd00156. REC. 1 hit.
Gene3Di3.40.50.180. 1 hit.
HAMAPiMF_00099. CheB_methylest. 1 hit.
InterProiView protein in InterPro
IPR035909. CheB_C.
IPR011006. CheY-like_superfamily.
IPR008248. Sig_transdc_resp-reg_CheB.
IPR000673. Sig_transdc_resp-reg_Me-estase.
IPR001789. Sig_transdc_resp-reg_receiver.
PfamiView protein in Pfam
PF01339. CheB_methylest. 1 hit.
PF00072. Response_reg. 1 hit.
PIRSFiPIRSF000876. RR_chemtxs_CheB. 1 hit.
SMARTiView protein in SMART
SM00448. REC. 1 hit.
SUPFAMiSSF52172. SSF52172. 1 hit.
SSF52738. SSF52738. 1 hit.
PROSITEiView protein in PROSITE
PS50122. CHEB. 1 hit.
PS50110. RESPONSE_REGULATORY. 1 hit.

Sequencei

Sequence statusi: Complete.

B0R4K0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEALVVDDS HFMRTVISDI LEDGGVDVVG TAENGARALD AVTDVQPDVI
60 70 80 90 100
TMDVEMPEMD GIEATAEIMR EQPTPILMVS ALTTEDADAT LEAMEKGAID
110 120 130 140 150
TFAKPGGTIS TELSGHSEEL VAAVERVASA DPTAGHDVEM EPASPPDATT
160 170 180 190 200
SEYADNPTLL IGASTGGPNV VESILASLPA EADFRVLIVQ HMPDQFTSRF
210 220 230 240 250
ADRLDAASQY DITEAEDGSR IGGGEGLVAR GDYHMRVSGY SNGRLRVRLD
260 270 280 290 300
QSERLHSVRP AIDVTFKSAA ERVTDPLVSV VLTGMGSDGA DGVRAVKDAG
310 320 330 340
GATLAQNEAT SAVFGIPERA IETGCVDDVL PVDQLTEAIA DSIRRTT
Length:347
Mass (Da):36,484
Last modified:April 8, 2008 - v1
Checksum:i9691873DC8EA113B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti295A → P in CAA60162 (PubMed:7556066).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X86407 Genomic DNA. Translation: CAA60162.1.
AM774415 Genomic DNA. Translation: CAP13665.1.
PIRiS58646.
RefSeqiWP_010902691.1. NC_010364.1.

Genome annotation databases

EnsemblBacteriaiCAP13665; CAP13665; OE_2416R.
GeneIDi5953631.
KEGGihsl:OE_2416R.

Similar proteinsi

Entry informationi

Entry nameiCHEB_HALS3
AccessioniPrimary (citable) accession number: B0R4K0
Secondary accession number(s): Q48300, Q9HQW6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 14, 2014
Last sequence update: April 8, 2008
Last modified: November 22, 2017
This is version 60 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome