B0R4D5 (B0R4D5_HALS3) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
DNA gyrase subunit A HAMAP MF_01897
EC=5.99.1.3 HAMAP MF_01897

Gene names

Name:	gyrA HAMAP MF_01897 EMBL CAP13600.1
Ordered Locus Names:	OE2304F

Organism

Halobacterium salinarum (strain ATCC 29341 / DSM 671 / R1) [Complete proteome] [HAMAP]

Taxonomic identifier

478009 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Halobacteria › Halobacteriales › Halobacteriaceae › Halobacterium

Protein attributes

Sequence length	824 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings By similarity. HAMAP MF_01897
Catalytic activity	ATP-dependent breakage, passage and rejoining of double-stranded DNA. HAMAP MF_01897
Subunit structure	Made up of two chains. The A chain is responsible for DNA breakage and rejoining; the B chain catalyzes ATP hydrolysis. The enzyme forms an A2B2 tetramer By similarity. HAMAP MF_01897
Subcellular location	Cytoplasm By similarity HAMAP MF_01897.
Sequence similarities	Belongs to the topoisomerase GyrA/ParC subunit family. HAMAP MF_01897

Ontologies

Keywords
Cellular component	Cytoplasm HAMAP MF_01897
Ligand	ATP-binding HAMAP MF_01897 DNA-binding HAMAP MF_01897 Nucleotide-binding
Molecular function	Isomerase Topoisomerase HAMAP MF_01897
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro DNA-dependent DNA replication Inferred from electronic annotation. Source: HAMAP regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP DNA topoisomerase (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	B0R4D5 [UniParc]. Last modified April 8, 2008. Version 1. Checksum: CB55755219FE09F0	FASTA	824	90,223
	10 20 30 40 50 60 MSSESPDVPD DVADRVKNVR VDEEMEQSYI DYAMSVIAGR ALPDVRDGLK PVHRRILYAM 70 80 90 100 110 120 HESGVTSGSG HRKSSNIVGD TMGDYHPHGD SPIYDALVRM AQDFSMRYPL VDGQGNFGSV 130 140 150 160 170 180 DGDPAAAMRY TEARMAPIAE ELLTDIEKDT VAFQANYDDR LTEPAVLPSS FPNLLVNGSS 190 200 210 220 230 240 GIAVGMSTNV PPHNLGEVID ATIHLIDTPD CTVLDLMEYV KGPDFPTAAN IVGRADVKQA 250 260 270 280 290 300 YQTGRGRVRM RAEYHVESNG RRDSIVITEL PYQQNKSTLV ERIADDVRDG KLEGITDLRD 310 320 330 340 350 360 ESDRNGVRIV IDLKQKANTD VVENRLLESH LEKTFSVINL ALVDGEPKVL TLKELLAEYV 370 380 390 400 410 420 SHRKEVVRRR SEHDLGEAED RAHILDGRLT ALENADEVVE LIQDAEDRDA AKETLKSAFD 430 440 450 460 470 480 FSQDQADHIV RMQLGSLTSM EAADIESEYE DVTARIERLE EILGSEQELL DVIKDELREI 490 500 510 520 530 540 KTQYADERRT SFIEDTGTVA DEDLIPEEDT VVVLSEGDYI KRVPAETFDA QHRGGKGIIG 550 560 570 580 590 600 SDLKDGDRVS TVFTASTHDY LLCFTDQGQV YRLKVYQVPE MSRTARGTSA VNILDLDDGE 610 620 630 640 650 660 EISAVVTADD LDFKADDEYL TMATRNGYVK RTSVGEFGNI LSTGIIAIDL EDGDALADVE 670 680 690 700 710 720 VTDGSHDVIL GSEAGMAIRF DEGDVRAMGR NARGVRGMDL DAADRIAGVA AVESEDDRSL 730 740 750 760 770 780 LTVTEFGYGK RTRVGEYRSQ SRNGKGLVDI KTGDRNGDVV SVDAVGDDDS LVVMSADGQI 790 800 810 820 IQMPVDEIST VGRNTKGVNI MAVGSGDEVA GVSVRAADQA DGEE

References

[1]

"Evolution in the laboratory: the genome of Halobacterium salinarum strain R1 compared to that of strain NRC-1." Pfeiffer F., Schuster S.C., Broicher A., Falb M., Palm P., Rodewald K., Ruepp A., Soppa J., Tittor J., Oesterhelt D. Genomics 91:335-346(2008) [PubMed: 18313895] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29341 / DSM 671 / R1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM774415 Genomic DNA. Translation: CAP13600.1.
RefSeq	YP_001688948.1. NC_010364.1.
3D structure databases
ProteinModelPortal	B0R4D5.
ModBase	Search...
Protein-protein interaction databases
STRING	B0R4D5.
Proteomic databases
PRIDE	B0R4D5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5953841.
GenomeReviews	Gene locus OE2304F in contig AM774415_GR.
KEGG	hsl:OE2304F.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG635183.
OMA	TGRGRIY.
PhylomeDB	B0R4D5.
ProtClustDB	PRK05560.
Family and domain databases
HAMAP	MF_01897. GyrA. [Tree]
InterPro	IPR005743. GyrA. IPR006691. GyrA/parC_pinwhl. IPR002205. Topo_IIA_A/C. IPR013758. Topo_IIA_A/C_ab. IPR013760. Topo_IIA_cen. [Graphical view]
Gene3D	G3DSA:3.90.199.10. Topo_IIA_A/C_ab. 2 hits.
KO	K02469.
Pfam	PF03989. DNA_gyraseA_C. 6 hits. PF00521. DNA_topoisoIV. 1 hit. [Graphical view]
SMART	SM00434. TOP4c. 1 hit. [Graphical view]
SUPFAM	SSF56719. Topo_IIA_cen. 1 hit.
TIGRFAMs	TIGR01063. GyrA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

B0R4D5_HALS3

Accession

Primary (citable) accession number: B0R4D5

Entry history

Integrated into UniProtKB/TrEMBL:	April 8, 2008
Last sequence update:	April 8, 2008
Last modified:	January 25, 2012
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)