B0R4C9 (DNLI_HALS3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA ligase
EC=6.5.1.1

Alternative name(s):
Polydeoxyribonucleotide synthase [ATP]

Gene names

Name:	lig
Ordered Locus Names:	OE2298F

Organism

Halobacterium salinarum (strain ATCC 29341 / DSM 671 / R1) [Complete proteome] [HAMAP]

Taxonomic identifier

478009 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Halobacteria › Halobacteriales › Halobacteriaceae › Halobacterium

Protein attributes

Sequence length	561 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair By similarity. HAMAP MF_00407
Catalytic activity	ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m). HAMAP MF_00407
Cofactor	Divalent metal cations By similarity. HAMAP MF_00407
Sequence similarities	Belongs to the ATP-dependent DNA ligase family.

Ontologies

Keywords
Biological process	Cell cycle Cell division DNA damage DNA recombination DNA repair DNA replication
Ligand	ATP-binding Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA ligation involved in DNA repair Inferred from electronic annotation. Source: InterPro DNA recombination Inferred from electronic annotation. Source: UniProtKB-KW DNA replication Inferred from electronic annotation. Source: UniProtKB-KW cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA binding Inferred from electronic annotation. Source: InterPro DNA ligase (ATP) activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 561	561	DNA ligase HAMAP MF_00407		PRO_0000365245
Sites
	Active site	255	1	N6-AMP-lysine intermediate By similarity
	Binding site	253	1	ATP By similarity
	Binding site	260	1	ATP By similarity
	Binding site	275	1	ATP By similarity
	Binding site	304	1	ATP By similarity
	Binding site	344	1	ATP By similarity
	Binding site	421	1	ATP By similarity
	Binding site	427	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	B0R4C9 [UniParc]. Last modified April 8, 2008. Version 1. Checksum: 5BB30D3C1D106D2E	FASTA	561	59,620
	10 20 30 40 50 60 MKFGSFATFA ADIEATDADL DVVALVAELF DAADSDLDVV ARFVQGRVFP AHSETKLDIG 70 80 90 100 110 120 PQLCYTALSR AAGRNVDADD IEARLAETGD IGAVAGSLDL GGQTGLAAFG GGDDHADGLT 130 140 150 160 170 180 VSGVADELAA LAAAAGDGSQ SEKVTLLFGL FNQCTSREAR YLARLVLGEM RIGVGEGAVR 190 200 210 220 230 240 DAIAEAFDVP TAAVQRALQV SNDYGLVAET ARDSGTDALD AMGLEVGRPV QAMLAQAGTV 250 260 270 280 290 300 TDALDEWHEA AVETKFDGAR VQVHYDGDDV VLYSRNMENV TGALPELVEF VENNVTAPVI 310 320 330 340 350 360 IDGEAVAADE DGDPLPFQEI LKRFRRKHDV AAMREEISVE LHAFDCLHAP SADGGEDLLD 370 380 390 400 410 420 APFSDRHRRL RSVVDDASAV SEVLVTDDAD EIAAFEATAL EGGHEGIMLK NPAAPYTPGD 430 440 450 460 470 480 RGKDWLKRKP DVETLDLVVT GAEWGEGRRA SVLGTFLLSA RDAAGDGFET IGKVATGLTD 490 500 510 520 530 540 EELAALSDRL EPHVRSEDGQ TVDIEPAVVL EVGYEEIQAS PTYSSGYALR FPRFVSVRED 550 560 KTPTTADTIE RVERLAAQQQ Q

References

[1]

"Evolution in the laboratory: the genome of Halobacterium salinarum strain R1 compared to that of strain NRC-1." Pfeiffer F., Schuster S.C., Broicher A., Falb M., Palm P., Rodewald K., Ruepp A., Soppa J., Tittor J., Oesterhelt D. Genomics 91:335-346(2008) [PubMed: 18313895] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29341 / DSM 671 / R1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM774415 Genomic DNA. Translation: CAP13594.1.
RefSeq	YP_001688942.1. NC_010364.1.
3D structure databases
ProteinModelPortal	B0R4C9.
ModBase	Search...
Protein-protein interaction databases
STRING	B0R4C9.
Proteomic databases
PRIDE	B0R4C9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5954142.
GenomeReviews	Gene locus OE2298F in contig AM774415_GR.
KEGG	hsl:OE2298F.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG570821.
OMA	GRPRPFQ.
PhylomeDB	B0R4C9.
ProtClustDB	CLSK511253.
Enzyme and pathway databases
BioCyc	HSAL478009:OE2298F-MONOMER.
Family and domain databases
HAMAP	MF_00407. DNA_ligase. [Tree]
InterPro	IPR022865. DNA_ligae_ATP-dep_bac/arc. IPR000977. DNA_ligase_ATP-dep. IPR012309. DNA_ligase_ATP-dep_C. IPR012310. DNA_ligase_ATP-dep_cent. IPR016059. DNA_ligase_ATP-dep_CS. IPR012308. DNA_ligase_ATP-dep_N. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. [Graphical view]
Gene3D	G3DSA:1.10.3260.10. DNA_ligase_ATP-dep_N. 1 hit. G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
KO	K10747.
Pfam	PF04679. DNA_ligase_A_C. 1 hit. PF01068. DNA_ligase_A_M. 1 hit. PF04675. DNA_ligase_A_N. 1 hit. [Graphical view]
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit. SSF117018. SSF117018. 1 hit.
TIGRFAMs	TIGR00574. Dnl1. 1 hit.
PROSITE	PS00697. DNA_LIGASE_A1. 1 hit. PS00333. DNA_LIGASE_A2. False negative. PS50160. DNA_LIGASE_A3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNLI_HALS3

Accession

Primary (citable) accession number: B0R4C9

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 3, 2009
Last sequence update:	April 8, 2008
Last modified:	December 14, 2011
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program