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B0R414

- RTCA_HALS3

UniProt

B0R414 - RTCA_HALS3

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Protein

RNA 3'-terminal phosphate cyclase

Gene

rtcA

Organism
Halobacterium salinarum (strain ATCC 29341 / DSM 671 / R1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing.UniRule annotation

Catalytic activityi

ATP + RNA 3'-terminal-phosphate = AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei109 – 1091ATPUniRule annotation
Active sitei310 – 3101Tele-AMP-histidine intermediateUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi286 – 2905ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. RNA-3'-phosphate cyclase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. RNA processing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciHSAL478009:GJVF-611-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
RNA 3'-terminal phosphate cyclaseUniRule annotation (EC:6.5.1.4UniRule annotation)
Short name:
RNA cyclaseUniRule annotation
Short name:
RNA-3'-phosphate cyclaseUniRule annotation
Gene namesi
Name:rtcAUniRule annotation
Ordered Locus Names:OE_2081R
OrganismiHalobacterium salinarum (strain ATCC 29341 / DSM 671 / R1)
Taxonomic identifieri478009 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaHalobacteriaHalobacterialesHalobacteriaceaeHalobacterium
ProteomesiUP000001321: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 339339RNA 3'-terminal phosphate cyclasePRO_1000099348Add
BLAST

Proteomic databases

PRIDEiB0R414.

Interactioni

Protein-protein interaction databases

STRINGi478009.OE2081R.

Structurei

3D structure databases

ProteinModelPortaliB0R414.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0430.
HOGENOMiHOG000015264.
KOiK01974.
OMAiTTHIETC.

Family and domain databases

Gene3Di3.30.360.20. 1 hit.
3.65.10.20. 2 hits.
HAMAPiMF_00200. RTC.
InterProiIPR013791. RNA3'-term_phos_cycl_insert.
IPR023797. RNA3'_phos_cyclase_dom.
IPR000228. RNA3'_term_phos_cyc.
IPR017770. RNA3'_term_phos_cyc_type_1.
IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b.
[Graphical view]
PANTHERiPTHR11096. PTHR11096. 1 hit.
PfamiPF01137. RTC. 1 hit.
PF05189. RTC_insert. 1 hit.
[Graphical view]
SUPFAMiSSF52913. SSF52913. 1 hit.
SSF55205. SSF55205. 1 hit.
TIGRFAMsiTIGR03399. RNA_3prim_cycl. 1 hit.

Sequencei

Sequence statusi: Complete.

B0R414-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MHVLDGSSGG GQLVRTALTC AAVSGESFRM RYVRRARSNP GLQAQHCAAV
60 70 80 90 100
NAVADICDAA TDGVEVGSEA FSFEPEVAAE EADDDEEPTL GGTTSVEVGT
110 120 130 140 150
AGSIPLVFDS LLPLAGALDE PITATLTGGT DAKWAPPMDY FQHVKLPLLR
160 170 180 190 200
EHGIDATVSV DRRGFYPRGG GEATLTVEPS TPTPITLTER GDREALTAYS
210 220 230 240 250
VAESSLADDE VAEQQATAAA PDAAHEIAYT DADSAGSAVV LAAEYEHSRA
260 270 280 290 300
GFAALGERGV SADAVGENAA DALAAFESGP GAVDSHLADQ LVPVVAVAGG
310 320 330
EVRAPEVTTH IETCVDLLAE FDYDIDIEHT DDGAVVLSA
Length:339
Mass (Da):34,879
Last modified:April 8, 2008 - v1
Checksum:i27B75201F22A61FE
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM774415 Genomic DNA. Translation: CAP13479.1.
RefSeqiWP_012289214.1. NC_010364.1.
YP_001688827.1. NC_010364.1.

Genome annotation databases

EnsemblBacteriaiCAP13479; CAP13479; OE_2081R.
GeneIDi5952667.
KEGGihsl:OE2081R.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM774415 Genomic DNA. Translation: CAP13479.1 .
RefSeqi WP_012289214.1. NC_010364.1.
YP_001688827.1. NC_010364.1.

3D structure databases

ProteinModelPortali B0R414.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 478009.OE2081R.

Proteomic databases

PRIDEi B0R414.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAP13479 ; CAP13479 ; OE_2081R .
GeneIDi 5952667.
KEGGi hsl:OE2081R.

Phylogenomic databases

eggNOGi COG0430.
HOGENOMi HOG000015264.
KOi K01974.
OMAi TTHIETC.

Enzyme and pathway databases

BioCyci HSAL478009:GJVF-611-MONOMER.

Family and domain databases

Gene3Di 3.30.360.20. 1 hit.
3.65.10.20. 2 hits.
HAMAPi MF_00200. RTC.
InterProi IPR013791. RNA3'-term_phos_cycl_insert.
IPR023797. RNA3'_phos_cyclase_dom.
IPR000228. RNA3'_term_phos_cyc.
IPR017770. RNA3'_term_phos_cyc_type_1.
IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b.
[Graphical view ]
PANTHERi PTHR11096. PTHR11096. 1 hit.
Pfami PF01137. RTC. 1 hit.
PF05189. RTC_insert. 1 hit.
[Graphical view ]
SUPFAMi SSF52913. SSF52913. 1 hit.
SSF55205. SSF55205. 1 hit.
TIGRFAMsi TIGR03399. RNA_3prim_cycl. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Evolution in the laboratory: the genome of Halobacterium salinarum strain R1 compared to that of strain NRC-1."
    Pfeiffer F., Schuster S.C., Broicher A., Falb M., Palm P., Rodewald K., Ruepp A., Soppa J., Tittor J., Oesterhelt D.
    Genomics 91:335-346(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 29341 / DSM 671 / R1.

Entry informationi

Entry nameiRTCA_HALS3
AccessioniPrimary (citable) accession number: B0R414
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: April 8, 2008
Last modified: October 29, 2014
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3