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B0R414

- RTCA_HALS3

UniProt

B0R414 - RTCA_HALS3

Protein

RNA 3'-terminal phosphate cyclase

Gene

rtcA

Organism
Halobacterium salinarum (strain ATCC 29341 / DSM 671 / R1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 44 (01 Oct 2014)
      Sequence version 1 (08 Apr 2008)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing.UniRule annotation

    Catalytic activityi

    ATP + RNA 3'-terminal-phosphate = AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei109 – 1091ATPUniRule annotation
    Active sitei310 – 3101Tele-AMP-histidine intermediateUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi286 – 2905ATPUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. RNA-3'-phosphate cyclase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. RNA processing Source: InterPro

    Keywords - Molecular functioni

    Ligase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciHSAL478009:GJVF-611-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    RNA 3'-terminal phosphate cyclaseUniRule annotation (EC:6.5.1.4UniRule annotation)
    Short name:
    RNA cyclaseUniRule annotation
    Short name:
    RNA-3'-phosphate cyclaseUniRule annotation
    Gene namesi
    Name:rtcAUniRule annotation
    Ordered Locus Names:OE_2081R
    OrganismiHalobacterium salinarum (strain ATCC 29341 / DSM 671 / R1)
    Taxonomic identifieri478009 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaHalobacteriaHalobacterialesHalobacteriaceaeHalobacterium
    ProteomesiUP000001321: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 339339RNA 3'-terminal phosphate cyclasePRO_1000099348Add
    BLAST

    Proteomic databases

    PRIDEiB0R414.

    Interactioni

    Protein-protein interaction databases

    STRINGi478009.OE2081R.

    Structurei

    3D structure databases

    ProteinModelPortaliB0R414.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0430.
    HOGENOMiHOG000015264.
    KOiK01974.
    OMAiTTHIETC.

    Family and domain databases

    Gene3Di3.30.360.20. 1 hit.
    3.65.10.20. 2 hits.
    HAMAPiMF_00200. RTC.
    InterProiIPR013791. RNA3'-term_phos_cycl_insert.
    IPR023797. RNA3'_phos_cyclase_dom.
    IPR000228. RNA3'_term_phos_cyc.
    IPR017770. RNA3'_term_phos_cyc_type_1.
    IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b.
    [Graphical view]
    PANTHERiPTHR11096. PTHR11096. 1 hit.
    PfamiPF01137. RTC. 1 hit.
    PF05189. RTC_insert. 1 hit.
    [Graphical view]
    SUPFAMiSSF52913. SSF52913. 1 hit.
    SSF55205. SSF55205. 1 hit.
    TIGRFAMsiTIGR03399. RNA_3prim_cycl. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    B0R414-1 [UniParc]FASTAAdd to Basket

    « Hide

    MHVLDGSSGG GQLVRTALTC AAVSGESFRM RYVRRARSNP GLQAQHCAAV    50
    NAVADICDAA TDGVEVGSEA FSFEPEVAAE EADDDEEPTL GGTTSVEVGT 100
    AGSIPLVFDS LLPLAGALDE PITATLTGGT DAKWAPPMDY FQHVKLPLLR 150
    EHGIDATVSV DRRGFYPRGG GEATLTVEPS TPTPITLTER GDREALTAYS 200
    VAESSLADDE VAEQQATAAA PDAAHEIAYT DADSAGSAVV LAAEYEHSRA 250
    GFAALGERGV SADAVGENAA DALAAFESGP GAVDSHLADQ LVPVVAVAGG 300
    EVRAPEVTTH IETCVDLLAE FDYDIDIEHT DDGAVVLSA 339
    Length:339
    Mass (Da):34,879
    Last modified:April 8, 2008 - v1
    Checksum:i27B75201F22A61FE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM774415 Genomic DNA. Translation: CAP13479.1.
    RefSeqiYP_001688827.1. NC_010364.1.

    Genome annotation databases

    EnsemblBacteriaiCAP13479; CAP13479; OE_2081R.
    GeneIDi5952667.
    KEGGihsl:OE2081R.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM774415 Genomic DNA. Translation: CAP13479.1 .
    RefSeqi YP_001688827.1. NC_010364.1.

    3D structure databases

    ProteinModelPortali B0R414.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 478009.OE2081R.

    Proteomic databases

    PRIDEi B0R414.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAP13479 ; CAP13479 ; OE_2081R .
    GeneIDi 5952667.
    KEGGi hsl:OE2081R.

    Phylogenomic databases

    eggNOGi COG0430.
    HOGENOMi HOG000015264.
    KOi K01974.
    OMAi TTHIETC.

    Enzyme and pathway databases

    BioCyci HSAL478009:GJVF-611-MONOMER.

    Family and domain databases

    Gene3Di 3.30.360.20. 1 hit.
    3.65.10.20. 2 hits.
    HAMAPi MF_00200. RTC.
    InterProi IPR013791. RNA3'-term_phos_cycl_insert.
    IPR023797. RNA3'_phos_cyclase_dom.
    IPR000228. RNA3'_term_phos_cyc.
    IPR017770. RNA3'_term_phos_cyc_type_1.
    IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b.
    [Graphical view ]
    PANTHERi PTHR11096. PTHR11096. 1 hit.
    Pfami PF01137. RTC. 1 hit.
    PF05189. RTC_insert. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52913. SSF52913. 1 hit.
    SSF55205. SSF55205. 1 hit.
    TIGRFAMsi TIGR03399. RNA_3prim_cycl. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Evolution in the laboratory: the genome of Halobacterium salinarum strain R1 compared to that of strain NRC-1."
      Pfeiffer F., Schuster S.C., Broicher A., Falb M., Palm P., Rodewald K., Ruepp A., Soppa J., Tittor J., Oesterhelt D.
      Genomics 91:335-346(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 29341 / DSM 671 / R1.

    Entry informationi

    Entry nameiRTCA_HALS3
    AccessioniPrimary (citable) accession number: B0R414
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 24, 2009
    Last sequence update: April 8, 2008
    Last modified: October 1, 2014
    This is version 44 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3