B0R414 (RTCA_HALS3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
RNA 3'-terminal phosphate cyclase
Short name=RNA cyclase
Short name=RNA-3'-phosphate cyclase
EC=6.5.1.4

Gene names

Name:	rtcA
Ordered Locus Names:	OE2081R

Organism

Halobacterium salinarum (strain ATCC 29341 / DSM 671 / R1) [Complete proteome] [HAMAP]

Taxonomic identifier

478009 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Halobacteria › Halobacteriales › Halobacteriaceae › Halobacterium

Protein attributes

Sequence length	339 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing By similarity. HAMAP MF_00200
Catalytic activity	ATP + RNA 3'-terminal-phosphate = AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate. HAMAP MF_00200
Subcellular location	Cytoplasm Potential HAMAP MF_00200.
Sequence similarities	Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	RNA processing Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA-3'-phosphate cyclase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 339	339	RNA 3'-terminal phosphate cyclase HAMAP MF_00200		PRO_1000099348
Regions
	Nucleotide binding	286 – 290	5	ATP By similarity
Sites
	Active site	310	1	Tele-AMP-histidine intermediate By similarity
	Binding site	109	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	B0R414 [UniParc]. Last modified April 8, 2008. Version 1. Checksum: 27B75201F22A61FE	FASTA	339	34,879
	10 20 30 40 50 60 MHVLDGSSGG GQLVRTALTC AAVSGESFRM RYVRRARSNP GLQAQHCAAV NAVADICDAA 70 80 90 100 110 120 TDGVEVGSEA FSFEPEVAAE EADDDEEPTL GGTTSVEVGT AGSIPLVFDS LLPLAGALDE 130 140 150 160 170 180 PITATLTGGT DAKWAPPMDY FQHVKLPLLR EHGIDATVSV DRRGFYPRGG GEATLTVEPS 190 200 210 220 230 240 TPTPITLTER GDREALTAYS VAESSLADDE VAEQQATAAA PDAAHEIAYT DADSAGSAVV 250 260 270 280 290 300 LAAEYEHSRA GFAALGERGV SADAVGENAA DALAAFESGP GAVDSHLADQ LVPVVAVAGG 310 320 330 EVRAPEVTTH IETCVDLLAE FDYDIDIEHT DDGAVVLSA

References

[1]

"Evolution in the laboratory: the genome of Halobacterium salinarum strain R1 compared to that of strain NRC-1." Pfeiffer F., Schuster S.C., Broicher A., Falb M., Palm P., Rodewald K., Ruepp A., Soppa J., Tittor J., Oesterhelt D. Genomics 91:335-346(2008) [PubMed: 18313895] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29341 / DSM 671 / R1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM774415 Genomic DNA. Translation: CAP13479.1.
RefSeq	YP_001688827.1. NC_010364.1.
3D structure databases
ProteinModelPortal	B0R414.
ModBase	Search...
Protein-protein interaction databases
STRING	B0R414.
Proteomic databases
PRIDE	B0R414.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5952667.
GenomeReviews	Gene locus OE2081R in contig AM774415_GR.
KEGG	hsl:OE2081R.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG683539.
OMA	GGTDVAW.
PhylomeDB	B0R414.
ProtClustDB	PRK04204.
Enzyme and pathway databases
BioCyc	HSAL478009:OE2081R-MONOMER.
Family and domain databases
HAMAP	MF_00200. RTC. [Tree]
InterPro	IPR013791. RNA3'-term_phos_cycl_insert. IPR023797. RNA3'_phos_cyclase_dom. IPR000228. RNA3'_term_phos_cyc. IPR017770. RNA3'_term_phos_cyc_type_1. IPR013796. RNA3'_term_phos_cycl_insert. IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b. [Graphical view]
Gene3D	G3DSA:3.30.360.20. G3DSA:3.30.360.20. 1 hit. G3DSA:3.65.10.20. RNA3'_term_phos_cycl. 2 hits.
KO	K01974.
PANTHER	PTHR11096. RNA3'_term_phos_cycl. 1 hit.
Pfam	PF01137. RTC. 1 hit. PF05189. RTC_insert. 1 hit. [Graphical view]
SUPFAM	SSF52913. RNA3'-term_phos_cycl_insert. 1 hit. SSF55205. RNA3'_cycl/enolpyr_transf_A/B. 1 hit.
TIGRFAMs	TIGR03399. RNA_3prim_cycl. 1 hit.
PROSITE	PS01287. RTC. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RTCA_HALS3

Accession

Primary (citable) accession number: B0R414

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	April 8, 2008
Last modified:	December 14, 2011
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program