B0R3A3 (SYP_HALS3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Proline--tRNA ligase
EC=6.1.1.15

Alternative name(s):
Prolyl-tRNA synthetase
Short name=ProRS

Gene names

Name:	proS
Ordered Locus Names:	OE1595F

Organism

Halobacterium salinarum (strain ATCC 29341 / DSM 671 / R1) [Complete proteome] [HAMAP]

Taxonomic identifier

478009 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Halobacteria › Halobacteriales › Halobacteriaceae › Halobacterium

Protein attributes

Sequence length	501 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro) By similarity. HAMAP MF_01571
Catalytic activity	ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro). HAMAP MF_01571
Subunit structure	Homodimer By similarity. HAMAP MF_01571
Subcellular location	Cytoplasm By similarity HAMAP MF_01571.
Domain	Consists of three domains: the N-terminal catalytic domain, the anticodon-binding domain and the C-terminal extension By similarity. HAMAP MF_01571
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	prolyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW proline-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 501	501	Proline--tRNA ligase HAMAP MF_01571		PRO_1000215565

Sequences

	Sequence		Length	Mass (Da)
	B0R3A3 [UniParc]. Last modified April 8, 2008. Version 1. Checksum: 6ECAB1AB82DF1D03	FASTA	501	56,123
	10 20 30 40 50 60 MSDDDQELGI TESKEHSPGD WYAEVVQKAG LADYAPMGGF IVTRPRGYAL WEAIQDNLDG 70 80 90 100 110 120 WFKDTGVENA YFPMFIPEDY LEREKDIVEG FDPEVAWVTQ GGHDDLDQRL AVRPTSESII 130 140 150 160 170 180 APYLSQWVRS HRDLPLRVNQ WNSVVRWEAT ETKPFFRTKE FLWQEGHTAH ATDEAAWAET 190 200 210 220 230 240 TLRLDQYHRL YEDVLGIPVL RGRKPDHDKF PGADTTMSVE ALMPDGKSVQ GGTSHHLGQS 250 260 270 280 290 300 FADAFDITFA DEDEAERTAY TTSWGLSWRA IGALVMSHSD DQGLVLPPTV APKQVVIVPI 310 320 330 340 350 360 WQEDTKDDVE QYGAEIAAEL EAQGVRVHFD DRDGRNPGFK FNEWELNGVP VRFEIGPNEV 370 380 390 400 410 420 EDDEVTVVHR PDGESTVEDR AAIADRVHDH LDEVYDKLYD AAADRLAENV READNRADIL 430 440 450 460 470 480 GTIGQHGGYV KAPWCGDQDC EAEIKDQIAA EIVMVPLGED SAARAASELE GERVPEPDHD 490 500 GEDCAICGDE ATRTAYFAKS Y

References

[1]

"Evolution in the laboratory: the genome of Halobacterium salinarum strain R1 compared to that of strain NRC-1." Pfeiffer F., Schuster S.C., Broicher A., Falb M., Palm P., Rodewald K., Ruepp A., Soppa J., Tittor J., Oesterhelt D. Genomics 91:335-346(2008) [PubMed: 18313895] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29341 / DSM 671 / R1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM774415 Genomic DNA. Translation: CAP13217.1.
RefSeq	YP_001688566.1. NC_010364.1.
3D structure databases
ProteinModelPortal	B0R3A3.
ModBase	Search...
Protein-protein interaction databases
STRING	B0R3A3.
Proteomic databases
PRIDE	B0R3A3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5952517.
GenomeReviews	Gene locus OE1595F in contig AM774415_GR.
KEGG	hsl:OE1595F.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG334108.
OMA	KFAEYEL.
PhylomeDB	B0R3A3.
ProtClustDB	PRK08661.
Enzyme and pathway databases
BioCyc	HSAL478009:OE1595F-MONOMER.
Family and domain databases
HAMAP	MF_01571. Pro_tRNA_synth_type3. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II. IPR004154. Anticodon-bd. IPR002316. Pro-tRNA-synth_IIa. IPR004499. Pro-tRNA-synth_IIa_arc-type. IPR017449. Pro-tRNA_synth_II. IPR016061. Pro-tRNA_synth_II_C. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit. G3DSA:3.30.110.30. Pro-tRNA-synth_II_C_arc/euk. 1 hit.
KO	K01881.
PANTHER	PTHR11451:SF6. ProS_fam_I. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF09180. ProRS-C_1. 1 hit. PF00587. tRNA-synt_2b. 1 hit. [Graphical view]
PRINTS	PR01046. TRNASYNTHPRO.
SMART	SM00946. ProRS-C_1. 1 hit. [Graphical view]
SUPFAM	SSF52954. Anticodon_bd. 1 hit. SSF64586. Pro-tRNA_synth_II_C. 1 hit.
TIGRFAMs	TIGR00408. ProS_fam_I. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYP_HALS3

Accession

Primary (citable) accession number: B0R3A3

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 22, 2009
Last sequence update:	April 8, 2008
Last modified:	January 25, 2012
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program