B0KUP7 (TPMT_PSEPG) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thiopurine S-methyltransferase
EC=2.1.1.67

Alternative name(s):
Thiopurine methyltransferase

Gene names

Name:	tpm
Ordered Locus Names:	PputGB1_1445

Organism

Pseudomonas putida (strain GB-1) [Complete proteome] [HAMAP]

Taxonomic identifier

76869 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	216 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	S-adenosyl-L-methionine + a thiopurine = S-adenosyl-L-homocysteine + a thiopurine S-methylether. HAMAP MF_00812
Subcellular location	Cytoplasm By similarity HAMAP MF_00812.
Sequence similarities	Belongs to the methyltransferase superfamily. TPMT family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	thiopurine S-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 216	216	Thiopurine S-methyltransferase HAMAP MF_00812		PRO_1000083761
Sites
	Binding site	10	1	S-adenosyl-L-methionine By similarity
	Binding site	45	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	66	1	S-adenosyl-L-methionine By similarity
	Binding site	123	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	B0KUP7 [UniParc]. Last modified March 18, 2008. Version 1. Checksum: 65DC55B44ABF7E39	FASTA	216	24,358
	10 20 30 40 50 60 MEPAFWQKRW ADNQIGFHQA QVNPYLQKYW PRLQLAPASR VLVPLCGKSL DLAWLAGQGY 70 80 90 100 110 120 RVLGVELSRQ AVEGFFREHG LDADVLQHGA FEVWRSGEIE LWCGDFFTLQ AEDIADCVGL 130 140 150 160 170 180 YDRAALIALP PQMRAAYMRL LSAWLPAGCR GLLVTLDYDQ SLLGGPPFSV GDKEVLQGFA 190 200 210 GWQMDELEVV ELIQESPKFL QAGACSLLER VYRVKR

References

[1]

"Complete sequence of Pseudomonas putida GB-1." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Bruce D., Goodwin L., Chertkov O., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., McCarthy J.K., Richardson P. Submitted (JAN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: GB-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000926 Genomic DNA. Translation: ABY97352.1.
RefSeq	YP_001667688.1. NC_010322.1.
3D structure databases
ProteinModelPortal	B0KUP7.
ModBase	Search...
Protein-protein interaction databases
STRING	B0KUP7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5869210.
GenomeReviews	Gene locus PputGB1_1445 in contig CP000926_GR.
KEGG	ppg:PputGB1_1445.
PATRIC	19929879. VBIPsePut76638_1454.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG444929.
OMA	QGDIFTL.
ProtClustDB	PRK13255.
Enzyme and pathway databases
BioCyc	PPUT76869:PPUTGB1_1445-MONOMER.
Family and domain databases
HAMAP	MF_00812. Thiopur_methtran. [Tree]
InterPro	IPR022474. Thiopur_S-MeTfrase_Se/Te_detox. IPR008854. Thiopurine_S-MeTrfase. IPR016822. Thiopurine_S-MeTrfase_sub. [Graphical view]
KO	K00569.
PANTHER	PTHR10259. PTHR10259. 1 hit.
Pfam	PF05724. TPMT. 1 hit. [Graphical view]
PIRSF	PIRSF023956. Thiopurine_S-methyltransferase. 1 hit.
TIGRFAMs	TIGR03840. TMPT_Se_Te. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TPMT_PSEPG

Accession

Primary (citable) accession number: B0KUP7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	March 18, 2008
Last modified:	January 25, 2012
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program