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Protein

Lipid-A-disaccharide synthase

Gene

lpxB

Organism
Pseudomonas putida (strain GB-1)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Condensation of UDP-2,3-diacylglucosamine and 2,3-diacylglucosamine-1-phosphate to form lipid A disaccharide, a precursor of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell.UniRule annotation

Catalytic activityi

UDP-2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosamine + 2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl 1-phosphate = UDP + 2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-beta-D-glucosaminyl-(1->6)-2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl 1-phosphate.UniRule annotation

Pathwayi: lipid IV(A) biosynthesis

This protein is involved in step 5 of the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine.UniRule annotation
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (lpxA)
  2. UDP-3-O-acyl-N-acetylglucosamine deacetylase (lpxC)
  3. no protein annotated in this organism
  4. UDP-2,3-diacylglucosamine hydrolase (lpxH)
  5. Lipid-A-disaccharide synthase (lpxB)
  6. Tetraacyldisaccharide 4'-kinase (lpxK)
This subpathway is part of the pathway lipid IV(A) biosynthesis, which is itself part of Glycolipid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine, the pathway lipid IV(A) biosynthesis and in Glycolipid biosynthesis.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase
Biological processLipid A biosynthesis, Lipid biosynthesis, Lipid metabolism

Enzyme and pathway databases

BioCyciPPUT76869:G1GAZ-1205-MONOMER
UniPathwayiUPA00359; UER00481

Protein family/group databases

CAZyiGT19 Glycosyltransferase Family 19

Names & Taxonomyi

Protein namesi
Recommended name:
Lipid-A-disaccharide synthaseUniRule annotation (EC:2.4.1.182UniRule annotation)
Gene namesi
Name:lpxBUniRule annotation
Ordered Locus Names:PputGB1_1159
OrganismiPseudomonas putida (strain GB-1)
Taxonomic identifieri76869 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002157 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000802811 – 375Lipid-A-disaccharide synthaseAdd BLAST375

Interactioni

Protein-protein interaction databases

STRINGi76869.PputGB1_1159

Structurei

3D structure databases

ProteinModelPortaliB0KSB2
SMRiB0KSB2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LpxB family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D0V Bacteria
COG0763 LUCA
HOGENOMiHOG000018004
KOiK00748
OMAiPTVWAWR

Family and domain databases

HAMAPiMF_00392 LpxB, 1 hit
InterProiView protein in InterPro
IPR003835 Glyco_trans_19
PANTHERiPTHR30372 PTHR30372, 1 hit
PfamiView protein in Pfam
PF02684 LpxB, 1 hit
TIGRFAMsiTIGR00215 lpxB, 1 hit

Sequencei

Sequence statusi: Complete.

B0KSB2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQLCVALVA GEASGDILGS GLMRALKARH PDVRFIGVGG PLMEAEGLQS
60 70 80 90 100
YFPMERLAVM GLVEVLGRLR ELLKRRKLLI QTLIDEKPDV FIGIDAPDFT
110 120 130 140 150
LNIELKLRQA GIKTVHYVSP SVWAWRQKRV LKIREGCDLM LTLLPFEARF
160 170 180 190 200
YEEQGVPVRF VGHPLADTIP LEADRSVARA ALGLGEGPIV ALMPGSRGGE
210 220 230 240 250
VGRLGALFLD AAEHLCQQVP GVRFVLPCAN AARRAQVEHM LEGRQLPLTL
260 270 280 290 300
LDGQSHQALA ACDAVLIASG TATLEALLYK RPMVVAYRLA PLTYWILKRL
310 320 330 340 350
VKSPYVSLPN LLAQRELVPE LLQDQATSQA LANTLAPLVR DGSQQTERFD
360 370
EIHRTLRRDA SNQAAEAVLA LLKDR
Length:375
Mass (Da):41,202
Last modified:March 18, 2008 - v1
Checksum:i22E826CAC4A2E1A7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000926 Genomic DNA Translation: ABY97067.1
RefSeqiWP_012270846.1, NC_010322.1

Genome annotation databases

EnsemblBacteriaiABY97067; ABY97067; PputGB1_1159
KEGGippg:PputGB1_1159

Similar proteinsi

Entry informationi

Entry nameiLPXB_PSEPG
AccessioniPrimary (citable) accession number: B0KSB2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: March 18, 2008
Last modified: May 23, 2018
This is version 53 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

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