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B0KS97

- GLND_PSEPG

UniProt

B0KS97 - GLND_PSEPG

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Protein

Bifunctional uridylyltransferase/uridylyl-removing enzyme

Gene
glnD, PputGB1_1144
Organism
Pseudomonas putida (strain GB-1)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen assimilation and metabolism By similarity.UniRule annotation

Catalytic activityi

UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotation
Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Enzyme regulationi

Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity By similarity.UniRule annotation

GO - Molecular functioni

  1. [protein-PII] uridylyltransferase activity Source: UniProtKB-HAMAP
  2. amino acid binding Source: InterPro
  3. metal ion binding Source: InterPro
  4. phosphoric diester hydrolase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. nitrogen compound metabolic process Source: InterPro
  2. regulation of nitrogen utilization Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

BioCyciPPUT76869:GIXB-1174-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional uridylyltransferase/uridylyl-removing enzyme
Short name:
UTase/UR
Alternative name(s):
Bifunctional [protein-PII] modification enzyme
Bifunctional nitrogen sensor protein
Including the following 2 domains:
[Protein-PII] uridylyltransferase (EC:2.7.7.59)
Short name:
PII uridylyltransferase
Short name:
UTase
[Protein-PII]-UMP uridylyl-removing enzyme (EC:3.1.4.-)
Short name:
UR
Gene namesi
Name:glnD
Ordered Locus Names:PputGB1_1144
OrganismiPseudomonas putida (strain GB-1)
Taxonomic identifieri76869 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000002157: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 900900Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotationPRO_1000078808Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi76869.PputGB1_1144.

Structurei

3D structure databases

ProteinModelPortaliB0KS97.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini481 – 590110HDAdd
BLAST
Domaini706 – 78479ACT 1Add
BLAST
Domaini816 – 90085ACT 2Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 342342UridylyltransferaseUniRule annotationAdd
BLAST
Regioni343 – 705363Uridylyl-removingUniRule annotationAdd
BLAST

Domaini

Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing By similarity.UniRule annotation

Sequence similaritiesi

Belongs to the GlnD family.
Contains 2 ACT domains.
Contains 1 HD domain.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG2844.
HOGENOMiHOG000261778.
KOiK00990.
OMAiHHLLMSV.
OrthoDBiEOG6CCH44.

Family and domain databases

Gene3Di1.10.3210.10. 1 hit.
HAMAPiMF_00277. PII_uridylyl_transf.
InterProiIPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view]
PfamiPF01842. ACT. 1 hit.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
PROSITEiPS51671. ACT. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B0KS97-1 [UniParc]FASTAAdd to Basket

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MPQVDPELFD RGQFQAELAL KASPIAAFKK AIRLAGEVLD KRFRAGSEIR    50
PLIEARAWLV DNILQQAWNQ FDWGDQSGIA LVAVGGYGRG ELHPHSDIDL 100
LILLGAAEHE QYRDAIERFL TLLWDIGLEV GQSVRTVDEC AEQARADLTV 150
ITNLMESRTI CGPEALRQRM LEVTSTAHMW PSKEFFLAKR AELKARHHKY 200
NDTEYNLEPN VKGSPGGLRD IQTVLWVARR QYGTLNLHAL AGEGFLLESE 250
NELLASSQDF LWKVRYALHM LAGRAEDRLL FDHQRSLATL LGYGDDNPKR 300
AIEQFMQQYY RVVMSISQLC DLIVQHFEEV ILADEESGST QPLNARFRLH 350
DGYIEAANPN VFKRTPFAML EIFVLMAQRP EIKGVRADTV RLLREHRHLI 400
DDTFRTDIRN TSLFIELFKC EIGIHRNLRR MNRYGILGRY LPEFGLIVGQ 450
MQHDLFHIYT VDAHTLNLIK HLRKLQYTPV SEKFPLASKL MGRLPKPELI 500
YLAGLYHDIG KGRQGDHSEI GAVDAQKFCE RHQLPAWDSR LIVWLVQNHL 550
VMSTTAQRKD LSDPQVINDF ALHVGDETRL DYLYVLTVAD INATNPSLWN 600
SWRASLLRQL YTETKRALRR GLENPLDREE QIRQTQSSAL DILVREGTDP 650
DDVEQLWSQL GDDYFLKHNA ADVAWHTDAI LQQPADGGPL VLIKETTQRE 700
FEGGTQIFIY APDQHDFFAV TVAAMSQLNL NIHDARIITS SSQFTLDTYI 750
VLDNDGGSIG DNPQRVKQIR DGLTEALRNP EDYPTIIQRR VPRQLKHFDF 800
PPQVTILNDA QRPVTILEIT APDRPGLLAR LGRIFLEFDL SLQNAKIATL 850
GERVEDVFFI TDADNQPLSD PQLCSRLQEA IVQQLQAGQG SDTSPTRVTF 900
Length:900
Mass (Da):102,573
Last modified:March 18, 2008 - v1
Checksum:i7650D665F9A853D7
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000926 Genomic DNA. Translation: ABY97052.1.
RefSeqiYP_001667388.1. NC_010322.1.

Genome annotation databases

EnsemblBacteriaiABY97052; ABY97052; PputGB1_1144.
GeneIDi5868903.
KEGGippg:PputGB1_1144.
PATRICi19929261. VBIPsePut76638_1150.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000926 Genomic DNA. Translation: ABY97052.1 .
RefSeqi YP_001667388.1. NC_010322.1.

3D structure databases

ProteinModelPortali B0KS97.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 76869.PputGB1_1144.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABY97052 ; ABY97052 ; PputGB1_1144 .
GeneIDi 5868903.
KEGGi ppg:PputGB1_1144.
PATRICi 19929261. VBIPsePut76638_1150.

Phylogenomic databases

eggNOGi COG2844.
HOGENOMi HOG000261778.
KOi K00990.
OMAi HHLLMSV.
OrthoDBi EOG6CCH44.

Enzyme and pathway databases

BioCyci PPUT76869:GIXB-1174-MONOMER.

Family and domain databases

Gene3Di 1.10.3210.10. 1 hit.
HAMAPi MF_00277. PII_uridylyl_transf.
InterProi IPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view ]
Pfami PF01842. ACT. 1 hit.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view ]
PIRSFi PIRSF006288. PII_uridyltransf. 1 hit.
SMARTi SM00471. HDc. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01693. UTase_glnD. 1 hit.
PROSITEi PS51671. ACT. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: GB-1.

Entry informationi

Entry nameiGLND_PSEPG
AccessioniPrimary (citable) accession number: B0KS97
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: March 18, 2008
Last modified: June 11, 2014
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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