Reviewed, UniProtKB/Swiss-Prot B0KS79 (PLSB_PSEPG)

Names and origin

Protein names

Recommended name:
    Glycerol-3-phosphate acyltransferase
      Short name=GPAT
    EC=2.3.1.15

Gene names

Name:	plsB
Ordered Locus Names:	PputGB1_1126

Organism

Pseudomonas putida (strain GB-1) [Complete proteome] [HAMAP]

Taxonomic identifier

76869 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	828 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Acyl-CoA + sn-glycerol 3-phosphate = CoA + 1-acyl-sn-glycerol 3-phosphate. HAMAP MF_00393
Pathway	Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 1/3. HAMAP MF_00393
Subcellular location	Cell membrane; Peripheral membrane protein By similarity.
Domain	The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate By similarity.
Sequence similarities	Belongs to the GPAT/DAPAT family.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Cell membrane Membrane
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phospholipid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	glycerol-3-phosphate O-acyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 828	828	Glycerol-3-phosphate acyltransferase HAMAP MF_00393		PRO_1000080288
Regions
	Motif	309 – 314	6	HXXXXD motif HAMAP MF_00393

Sequences

	Sequence		Length	Mass (Da)
	B0KS79-1 [UniParc]. Last modified March 18, 2008. Version 1. Checksum: 450923A7373B3BB4	FASTA	828	93,622
	10 20 30 40 50 60 MTRSPLHRLI FGGLRRLLYL WVRSETINQS SMTLNLDRSR PVFYALPSPS LTDLAVLDHE 70 80 90 100 110 120 CTKAGLPRPV LPVAVGPLQE PAAFFYLTPD PDWLGRQDKS GAPPTLERLV AAVSQHAEED 130 140 150 160 170 180 AQIIPVSVFW GQTPASESSP WKLLFADSWA VTGRLRRLLT VLILGRKTRV QFSAPIHLRE 190 200 210 220 230 240 LVQHNKGHAR TVRMAQRLMR VHFRNLKTAV IGPDISHRRN LVKGLVHAPL VRQAIADEAQ 250 260 270 280 290 300 RENLPLAKAE AQALRYGNEI ASDYTYTVIR FLEVVLSWFW NKIYDGIKVN HIEQVQGIAP 310 320 330 340 350 360 GHEVIYVPCH RSHIDYLLLS YLLFRNGLTP PHVAAGINLN MPVVGNLLRR GGAFFMRRTF 370 380 390 400 410 420 KGNPLYTAVF NEYLHTLYTK GFPVEYFVEG GRSRTGRMLQ PRTGMLAITL RSFLRSSRTP 430 440 450 460 470 480 IVFVPVYIGY ERVLEGRTYL GELRGASKKK ESVLDIFKVF GALKQRFGQV YVNFGEPIRL 490 500 510 520 530 540 AGFLDQQQPG WREQDHGPQY RPDWLNATTT RLGETVARHL NEAAAINPVN LVALALLSTS 550 560 570 580 590 600 RLALDERALT RVLDLYLALL RQVPYSPHTT LPDGDGQALI EHVRSMNLVA EQKDALGRIL 610 620 630 640 650 660 FLDEGNAVLM TYYRNNVLHI FALPALLASF FLSSSRMSRE LLGQYVHALY PYLQAELFLR 670 680 690 700 710 720 WAPEQLDEVI DQWLAALVEQ GLLRQENDVY VRPAPSSRQF VLLTLLARTI TQTLQRFYMA 730 740 750 760 770 780 TSLLINSGQN SLSAEALEDL CVMMAQRLSI LHGLNAPEFF DKTLFRHFIQ TLLQQGVLHT 790 800 810 820 DTQGKLGYHD KLGELAEGVA KRVLSAELRL SIRQVALHRD DGLESSTI

References

[1]

"Complete sequence of Pseudomonas putida GB-1." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Bruce D., Goodwin L., Chertkov O., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., McCarthy J.K., Richardson P. Submitted (JAN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000926 Genomic DNA. Translation: ABY97034.1.
RefSeq	YP_001667370.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5868885.
GenomeReviews	Gene locus PputGB1_1126 in contig CP000926_GR.
KEGG	ppg:PputGB1_1126.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	VPVYIGY.
Family and domain databases
HAMAP	MF_00393. [Tree]
InterPro	IPR002123. Acyltransferase. [Graphical view]
Pfam	PF01553. Acyltransferase. 1 hit. [Graphical view]
SMART	SM00563. PlsC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PLSB_PSEPG

Accession

Primary (citable) accession number: B0KS79

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	March 18, 2008
Last modified:	November 3, 2009
This is version 12 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)