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Protein

Glycerol-3-phosphate acyltransferase

Gene

plsB

Organism
Pseudomonas putida (strain GB-1)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Acyl-CoA + sn-glycerol 3-phosphate = CoA + 1-acyl-sn-glycerol 3-phosphate.UniRule annotation

Pathwayi: CDP-diacylglycerol biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes CDP-diacylglycerol from sn-glycerol 3-phosphate.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Glycerol-3-phosphate acyltransferase (plsB)
  2. no protein annotated in this organism
  3. Phosphatidate cytidylyltransferase (PputGB1_1151)
This subpathway is part of the pathway CDP-diacylglycerol biosynthesis, which is itself part of Phospholipid metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CDP-diacylglycerol from sn-glycerol 3-phosphate, the pathway CDP-diacylglycerol biosynthesis and in Phospholipid metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

Enzyme and pathway databases

BioCyciPPUT76869:GIXB-1156-MONOMER.
UniPathwayiUPA00557; UER00612.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycerol-3-phosphate acyltransferaseUniRule annotation (EC:2.3.1.15UniRule annotation)
Short name:
GPATUniRule annotation
Gene namesi
Name:plsBUniRule annotation
Ordered Locus Names:PputGB1_1126
OrganismiPseudomonas putida (strain GB-1)
Taxonomic identifieri76869 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002157 Componenti: Chromosome

Subcellular locationi

  • Cell inner membrane UniRule annotation; Peripheral membrane protein UniRule annotation; Cytoplasmic side UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 828828Glycerol-3-phosphate acyltransferasePRO_1000080288Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi76869.PputGB1_1126.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi309 – 3146HXXXXD motif

Domaini

The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.UniRule annotation

Sequence similaritiesi

Belongs to the GPAT/DAPAT family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105E55. Bacteria.
COG2937. LUCA.
HOGENOMiHOG000218231.
KOiK00631.
OMAiEVIYVPC.
OrthoDBiEOG6B625B.

Family and domain databases

HAMAPiMF_00393. Glyc3P_acyltrans.
InterProiIPR022284. GPAT/DHAPAT.
IPR028354. GPAT_PlsB.
IPR002123. Plipid/glycerol_acylTrfase.
[Graphical view]
PANTHERiPTHR12563. PTHR12563. 2 hits.
PfamiPF01553. Acyltransferase. 1 hit.
[Graphical view]
PIRSFiPIRSF500064. GPAT. 1 hit.
PIRSF000437. GPAT_DHAPAT. 1 hit.
SMARTiSM00563. PlsC. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03703. plsB. 1 hit.

Sequencei

Sequence statusi: Complete.

B0KS79-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTRSPLHRLI FGGLRRLLYL WVRSETINQS SMTLNLDRSR PVFYALPSPS
60 70 80 90 100
LTDLAVLDHE CTKAGLPRPV LPVAVGPLQE PAAFFYLTPD PDWLGRQDKS
110 120 130 140 150
GAPPTLERLV AAVSQHAEED AQIIPVSVFW GQTPASESSP WKLLFADSWA
160 170 180 190 200
VTGRLRRLLT VLILGRKTRV QFSAPIHLRE LVQHNKGHAR TVRMAQRLMR
210 220 230 240 250
VHFRNLKTAV IGPDISHRRN LVKGLVHAPL VRQAIADEAQ RENLPLAKAE
260 270 280 290 300
AQALRYGNEI ASDYTYTVIR FLEVVLSWFW NKIYDGIKVN HIEQVQGIAP
310 320 330 340 350
GHEVIYVPCH RSHIDYLLLS YLLFRNGLTP PHVAAGINLN MPVVGNLLRR
360 370 380 390 400
GGAFFMRRTF KGNPLYTAVF NEYLHTLYTK GFPVEYFVEG GRSRTGRMLQ
410 420 430 440 450
PRTGMLAITL RSFLRSSRTP IVFVPVYIGY ERVLEGRTYL GELRGASKKK
460 470 480 490 500
ESVLDIFKVF GALKQRFGQV YVNFGEPIRL AGFLDQQQPG WREQDHGPQY
510 520 530 540 550
RPDWLNATTT RLGETVARHL NEAAAINPVN LVALALLSTS RLALDERALT
560 570 580 590 600
RVLDLYLALL RQVPYSPHTT LPDGDGQALI EHVRSMNLVA EQKDALGRIL
610 620 630 640 650
FLDEGNAVLM TYYRNNVLHI FALPALLASF FLSSSRMSRE LLGQYVHALY
660 670 680 690 700
PYLQAELFLR WAPEQLDEVI DQWLAALVEQ GLLRQENDVY VRPAPSSRQF
710 720 730 740 750
VLLTLLARTI TQTLQRFYMA TSLLINSGQN SLSAEALEDL CVMMAQRLSI
760 770 780 790 800
LHGLNAPEFF DKTLFRHFIQ TLLQQGVLHT DTQGKLGYHD KLGELAEGVA
810 820
KRVLSAELRL SIRQVALHRD DGLESSTI
Length:828
Mass (Da):93,622
Last modified:March 18, 2008 - v1
Checksum:i450923A7373B3BB4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000926 Genomic DNA. Translation: ABY97034.1.
RefSeqiWP_012270815.1. NC_010322.1.

Genome annotation databases

EnsemblBacteriaiABY97034; ABY97034; PputGB1_1126.
KEGGippg:PputGB1_1126.
PATRICi19929225. VBIPsePut76638_1132.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000926 Genomic DNA. Translation: ABY97034.1.
RefSeqiWP_012270815.1. NC_010322.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi76869.PputGB1_1126.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABY97034; ABY97034; PputGB1_1126.
KEGGippg:PputGB1_1126.
PATRICi19929225. VBIPsePut76638_1132.

Phylogenomic databases

eggNOGiENOG4105E55. Bacteria.
COG2937. LUCA.
HOGENOMiHOG000218231.
KOiK00631.
OMAiEVIYVPC.
OrthoDBiEOG6B625B.

Enzyme and pathway databases

UniPathwayiUPA00557; UER00612.
BioCyciPPUT76869:GIXB-1156-MONOMER.

Family and domain databases

HAMAPiMF_00393. Glyc3P_acyltrans.
InterProiIPR022284. GPAT/DHAPAT.
IPR028354. GPAT_PlsB.
IPR002123. Plipid/glycerol_acylTrfase.
[Graphical view]
PANTHERiPTHR12563. PTHR12563. 2 hits.
PfamiPF01553. Acyltransferase. 1 hit.
[Graphical view]
PIRSFiPIRSF500064. GPAT. 1 hit.
PIRSF000437. GPAT_DHAPAT. 1 hit.
SMARTiSM00563. PlsC. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03703. plsB. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: GB-1.

Entry informationi

Entry nameiPLSB_PSEPG
AccessioniPrimary (citable) accession number: B0KS79
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: March 18, 2008
Last modified: November 11, 2015
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.