B0KP70 (ARGA_PSEPG) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Amino-acid acetyltransferase
EC=2.3.1.1

Alternative name(s):
N-acetylglutamate synthase
Short name=AGS
Short name=NAGS

Gene names

Name:	argA
Ordered Locus Names:	PputGB1_5245

Organism

Pseudomonas putida (strain GB-1) [Complete proteome] [HAMAP]

Taxonomic identifier

76869 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	432 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Acetyl-CoA + L-glutamate = CoA + N-acetyl-L-glutamate. HAMAP MF_01105
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 1/4. HAMAP MF_01105
Subcellular location	Cytoplasm By similarity HAMAP MF_01105.
Sequence similarities	Belongs to the acetyltransferase family. ArgA subfamily. Contains 1 N-acetyltransferase domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	acetyl-CoA:L-glutamate N-acetyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 432	432	Amino-acid acetyltransferase HAMAP MF_01105		PRO_1000084820
Regions
	Domain	286 – 425	140	N-acetyltransferase

Sequences

	Sequence		Length	Mass (Da)
	B0KP70 [UniParc]. Last modified March 18, 2008. Version 1. Checksum: 8605EAC52A88EDDC	FASTA	432	47,399
	10 20 30 40 50 60 MPDYVNWLRH ASPYINAHRD CTFVVMLPGD GVEHPNFGNI VHDLVLLHSL GVRLVLVHGS 70 80 90 100 110 120 RPQIESRLAD RGLTPHYHRG MRITDAATLD CVIDAVGALR LAIEARLSMD IAASPMQGSR 130 140 150 160 170 180 LRVASGNLVT ARPIGVLEGV DYHHTGEVRR VDRKGISRLL DERSIVLLSP LGYSPTGEIF 190 200 210 220 230 240 NLACEDVATR AAIELGADKL LLFGAEPGLL DADGKLVREL RPQQVAPHLQ RLGSDYQGEL 250 260 270 280 290 300 LDAAAEACKG GVARSHIVSY AEDGALLTEL FTRGGGGTLV SQEQFEVVRE ASIEDVGGLL 310 320 330 340 350 360 ELISPLEEQG ILVRRSREVL EREIEQFSVV EREGMIIACA ALYPIADSEA GELACLAVNP 370 380 390 400 410 420 EYRHGGRGDE LLERIESRAR QMGLNTLFVL TTRTAHWFRE RGFAPSGVER LPAARASLYN 430 YQRNSKIFEK AL

References

[1]

"Complete sequence of Pseudomonas putida GB-1." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Bruce D., Goodwin L., Chertkov O., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., McCarthy J.K., Richardson P. Submitted (JAN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: GB-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000926 Genomic DNA. Translation: ABZ01128.1.
RefSeq	YP_001671463.1. NC_010322.1.
3D structure databases
ProteinModelPortal	B0KP70.
ModBase	Search...
Protein-protein interaction databases
STRING	B0KP70.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5873059.
GenomeReviews	Gene locus PputGB1_5245 in contig CP000926_GR.
KEGG	ppg:PputGB1_5245.
PATRIC	19937569. VBIPsePut76638_5244.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG545264.
OMA	AFNLAME.
ProtClustDB	PRK05279.
Enzyme and pathway databases
BioCyc	PPUT76869:PPUTGB1_5245-MONOMER.
Family and domain databases
HAMAP	MF_01105. N-acetyl_glu_synth. [Tree]
InterPro	IPR000182. AcTrfase_GCN5-related_dom. IPR016181. Acyl_CoA_acyltransferase. IPR001048. Asp/Glu/Uridylate_kinase. IPR010167. NH2A_AcTrfase_ArgA. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit. G3DSA:3.40.630.30. Acyl_CoA_acyltransferase. 1 hit.
KO	K14682.
Pfam	PF00696. AA_kinase. 1 hit. PF00583. Acetyltransf_1. 1 hit. [Graphical view]
PIRSF	PIRSF000423. ArgA. 1 hit.
SUPFAM	SSF53633. Aa_kinase. 1 hit. SSF55729. Acyl_CoA_acyltransferase. 1 hit.
TIGRFAMs	TIGR01890. N-Ac-Glu-synth. 1 hit.
PROSITE	PS51186. GNAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ARGA_PSEPG

Accession

Primary (citable) accession number: B0KP70

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	March 18, 2008
Last modified:	January 25, 2012
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program