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B0KN57

- TRMB_PSEPG

UniProt

B0KN57 - TRMB_PSEPG

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Protein
tRNA (guanine-N(7)-)-methyltransferase
Gene
trmB, PputGB1_5153
Organism
Pseudomonas putida (strain GB-1)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei70 – 701S-adenosyl-L-methionine By similarity
Binding sitei95 – 951S-adenosyl-L-methionine By similarity
Binding sitei122 – 1221S-adenosyl-L-methionine By similarity
Active sitei145 – 1451 By similarity
Binding sitei145 – 1451S-adenosyl-L-methionine By similarity
Binding sitei149 – 1491Substrate By similarity
Binding sitei181 – 1811Substrate By similarity

GO - Molecular functioni

  1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciPPUT76869:GIXB-5238-MONOMER.
    UniPathwayiUPA00989.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA (guanine-N(7)-)-methyltransferase (EC:2.1.1.33)
    Alternative name(s):
    tRNA (guanine(46)-N(7))-methyltransferase
    tRNA(m7G46)-methyltransferase
    Gene namesi
    Name:trmB
    Ordered Locus Names:PputGB1_5153
    OrganismiPseudomonas putida (strain GB-1)
    Taxonomic identifieri76869 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
    ProteomesiUP000002157: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 240240tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation
    PRO_1000084447Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi76869.PputGB1_5153.

    Structurei

    3D structure databases

    ProteinModelPortaliB0KN57.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni218 – 2214Substrate binding By similarity

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0220.
    HOGENOMiHOG000073968.
    KOiK03439.
    OMAiHSTLEMA.
    OrthoDBiEOG6K6VBC.

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    HAMAPiMF_01057. tRNA_methyltr_TrmB.
    InterProiIPR029063. SAM-dependent_MTases-like.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view]
    PfamiPF02390. Methyltransf_4. 1 hit.
    [Graphical view]
    SUPFAMiSSF53335. SSF53335. 1 hit.
    TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
    PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B0KN57-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTESHDTPIT PDGEARPHRR IKSFVMRAGR MTEGQQRGLD QGGPLYILPL    50
    ADSPVDYDQV FGRSAPRTLE IGFGMGHSLL EMAAAAPEQD FIGVEVHRPG 100
    VGALLNGVLT QGLKNLRVYD CDAIEVLNRC VADNSLDRLM LFFPDPWHKA 150
    RHHKRRIVQL EFAELVRRKL KPGGVFHMAT DWEPYAEYML EVMSAAPGYR 200
    NRAADGTYVP RPEERPITKF ERRGERLGHG VWDLKFEKVD 240
    Length:240
    Mass (Da):27,203
    Last modified:March 18, 2008 - v1
    Checksum:iEBF9DFED0FBC25EA
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000926 Genomic DNA. Translation: ABZ01038.1.
    RefSeqiYP_001671373.1. NC_010322.1.

    Genome annotation databases

    EnsemblBacteriaiABZ01038; ABZ01038; PputGB1_5153.
    GeneIDi5872968.
    KEGGippg:PputGB1_5153.
    PATRICi19937389. VBIPsePut76638_5154.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000926 Genomic DNA. Translation: ABZ01038.1 .
    RefSeqi YP_001671373.1. NC_010322.1.

    3D structure databases

    ProteinModelPortali B0KN57.
    ModBasei Search...

    Protein-protein interaction databases

    STRINGi 76869.PputGB1_5153.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABZ01038 ; ABZ01038 ; PputGB1_5153 .
    GeneIDi 5872968.
    KEGGi ppg:PputGB1_5153.
    PATRICi 19937389. VBIPsePut76638_5154.

    Phylogenomic databases

    eggNOGi COG0220.
    HOGENOMi HOG000073968.
    KOi K03439.
    OMAi HSTLEMA.
    OrthoDBi EOG6K6VBC.

    Enzyme and pathway databases

    UniPathwayi UPA00989 .
    BioCyci PPUT76869:GIXB-5238-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    HAMAPi MF_01057. tRNA_methyltr_TrmB.
    InterProi IPR029063. SAM-dependent_MTases-like.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view ]
    Pfami PF02390. Methyltransf_4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53335. SSF53335. 1 hit.
    TIGRFAMsi TIGR00091. TIGR00091. 1 hit.
    PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: GB-1.

    Entry informationi

    Entry nameiTRMB_PSEPG
    AccessioniPrimary (citable) accession number: B0KN57
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 20, 2008
    Last sequence update: March 18, 2008
    Last modified: June 11, 2014
    This is version 42 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

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