B0KN43 (SYR_PSEPG) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Arginine--tRNA ligase
EC=6.1.1.19

Alternative name(s):
Arginyl-tRNA synthetase
Short name=ArgRS

Gene names

Name:	argS
Ordered Locus Names:	PputGB1_5139

Organism

Pseudomonas putida (strain GB-1) [Complete proteome] [HAMAP]

Taxonomic identifier

76869 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas putida group › Pseudomonas putida

Protein attributes

Sequence length	578 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-tRNA(Arg). HAMAP-Rule MF_00123
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	arginyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP arginine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 578	578	Arginine--tRNA ligase HAMAP-Rule MF_00123		PRO_1000076224
Regions
	Motif	127 – 137	11	"HIGH" region HAMAP-Rule MF_00123

Sequences

	Sequence		Length	Mass (Da)
	B0KN43 [UniParc]. Last modified March 18, 2008. Version 1. Checksum: F106A5747B9C2FF5	FASTA	578	63,776
	10 20 30 40 50 60 MKDTIRQLIQ QALTQLVNDG VLPEGLSPAI QVENARDKTH GDFASNIAMM LAKPAGMKPR 70 80 90 100 110 120 DLAEKLINAL PASADISKVE IAGPGFLNFF QNTDALANRL DAALADARLG VRKAGPSEKV 130 140 150 160 170 180 VIDMSAPNLA KEMHVGHLRS TIIGDSVARV LEFLGDNVIR QNHVGDWGTQ FGMLMAYLQE 190 200 210 220 230 240 NPITSDELSD LENFYRAAKK RFDESEEFAT RARGLVVKLQ AGDPECLALW TRFKDISLSH 250 260 270 280 290 300 CQKTYELLNV KLTMADVMGE SAYNDDLANV VADLKAKGLL VESQGAQCVF LEEFKNSEGE 310 320 330 340 350 360 PLPVIVQKAD GGYLYATTDL AAVRYRSNVL NADRALYFVD QRQALHFNQV FEVARRAGFV 370 380 390 400 410 420 GHPMQMEHMG FGTMNGADGR PFKTRDGGTV KLIDLLTEAK ERAYALVKEK NPSLTEAELR 430 440 450 460 470 480 HIGEVVGIGA VKYADLSKHR TSDYSFNFEL MLNFEGNTAP YLLYAYTRVA GVFRKLGKGF 490 500 510 520 530 540 DEVEGNIVLQ AAHEQDLAAR LAQFGEILNN VADKGTPHVL CSYLYDLAGL FSSFYENCPI 550 560 570 LAAETLEQQQ SRLRLAALTG RTLKQGLELL GLETLERM

References

[1]

"Complete sequence of Pseudomonas putida GB-1." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Bruce D., Goodwin L., Chertkov O., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., McCarthy J.K., Richardson P. Submitted (JAN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: GB-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000926 Genomic DNA. Translation: ABZ01024.1.
RefSeq	YP_001671359.1. NC_010322.1.
3D structure databases
ProteinModelPortal	B0KN43.
ModBase	Search...
Protein-protein interaction databases
STRING	76869.PputGB1_5139.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABZ01024; ABZ01024; PputGB1_5139.
GeneID	5872954.
KEGG	ppg:PputGB1_5139.
PATRIC	19937359. VBIPsePut76638_5139.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0018.
HOGENOM	HOG000247212.
KO	K01887.
OMA	QGAKCVF.
ProtClustDB	PRK01611.
Enzyme and pathway databases
BioCyc	PPUT76869:GIXB-5224-MONOMER.
Family and domain databases
Gene3D	3.30.1360.70. 1 hit. 3.40.50.620. 1 hit.
HAMAP	MF_00123. Arg_tRNA_synth.
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR001278. Arg-tRNA-ligase_Ia. IPR015945. Arg-tRNA-synth_Ia_core. IPR005148. Arg-tRNA-synth_N. IPR008909. DALR_anticod-bd. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. [Graphical view]
PANTHER	PTHR11956. PTHR11956. 1 hit.
Pfam	PF03485. Arg_tRNA_synt_N. 1 hit. PF05746. DALR_1. 1 hit. PF00750. tRNA-synt_1d. 1 hit. [Graphical view]
PRINTS	PR01038. TRNASYNTHARG.
SMART	SM01016. Arg_tRNA_synt_N. 1 hit. SM00836. DALR_1. 1 hit. [Graphical view]
SUPFAM	SSF55190. Arg-tRNA-synth_Ic_N. 1 hit. SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMs	TIGR00456. argS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYR_PSEPG

Accession

Primary (citable) accession number: B0KN43

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	March 18, 2008
Last modified:	May 1, 2013
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program