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Protein

Malate dehydrogenase

Gene

mdh

Organism
Pseudomonas putida (strain GB-1)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the reversible oxidation of malate to oxaloacetate.UniRule annotation

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei33NADUniRule annotation1
Binding sitei82SubstrateUniRule annotation1
Binding sitei88SubstrateUniRule annotation1
Binding sitei95NADUniRule annotation1
Binding sitei120SubstrateUniRule annotation1
Binding sitei151SubstrateUniRule annotation1
Active sitei175Proton acceptorUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi8 – 13NADUniRule annotation6
Nucleotide bindingi118 – 120NADUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

NAD

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenaseUniRule annotation (EC:1.1.1.37UniRule annotation)
Gene namesi
Name:mdhUniRule annotation
Ordered Locus Names:PputGB1_0697
OrganismiPseudomonas putida (strain GB-1)
Taxonomic identifieri76869 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002157 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001916531 – 309Malate dehydrogenaseAdd BLAST309

Interactioni

Protein-protein interaction databases

STRINGi76869.PputGB1_0697.

Structurei

3D structure databases

ProteinModelPortaliB0KMD1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. MDH type 3 family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C80. Bacteria.
COG0039. LUCA.
HOGENOMiHOG000213794.
KOiK00024.
OMAiMDLMQTA.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_00487. Malate_dehydrog_3. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR011275. Malate_DH_type3.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.

Sequencei

Sequence statusi: Complete.

B0KMD1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKLTIVGAG LVGEAAAQII ARDELCRELV LMDVQGELAQ GKALDVWQAA
60 70 80 90 100
VESGSDTRVY GGAKAEMLDG SDLVVITAGV PRKPGQSRQD VLSINLPILD
110 120 130 140 150
GIMTDIKHHA PAATVLVVSN PVDVLTYRAW SVSGLGRDKV FGQAGVLDTA
160 170 180 190 200
RMKCFIAEQT GFSAKDITAL VLGGHGDSMV PLMRYCQIGS VPLSHFLSSE
210 220 230 240 250
QIEQIVERTR KGGGEILGLK KMGSACDAPG VAIAQMVDAI ANGRNRILPA
260 270 280 290 300
VAILQGEYGR KDIAMGVPCV LADEGLARVI ELPLDAQEQA MFDQSADQVA

RDIDEMKAL
Length:309
Mass (Da):32,718
Last modified:March 18, 2008 - v1
Checksum:iDB218AAD31586E46
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000926 Genomic DNA. Translation: ABY96607.1.
RefSeqiWP_012270412.1. NC_010322.1.

Genome annotation databases

EnsemblBacteriaiABY96607; ABY96607; PputGB1_0697.
KEGGippg:PputGB1_0697.
PATRICi19928351. VBIPsePut76638_0698.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000926 Genomic DNA. Translation: ABY96607.1.
RefSeqiWP_012270412.1. NC_010322.1.

3D structure databases

ProteinModelPortaliB0KMD1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi76869.PputGB1_0697.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABY96607; ABY96607; PputGB1_0697.
KEGGippg:PputGB1_0697.
PATRICi19928351. VBIPsePut76638_0698.

Phylogenomic databases

eggNOGiENOG4105C80. Bacteria.
COG0039. LUCA.
HOGENOMiHOG000213794.
KOiK00024.
OMAiMDLMQTA.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_00487. Malate_dehydrog_3. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR011275. Malate_DH_type3.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMDH_PSEPG
AccessioniPrimary (citable) accession number: B0KMD1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 18, 2008
Last modified: November 2, 2016
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.