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B0KM84

- SYI_PSEPG

UniProt

B0KM84 - SYI_PSEPG

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Pseudomonas putida (strain GB-1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 46 (01 Oct 2014)
      Sequence version 1 (18 Mar 2008)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei567 – 5671Aminoacyl-adenylateUniRule annotation
    Binding sitei611 – 6111ATPUniRule annotation
    Metal bindingi906 – 9061ZincUniRule annotation
    Metal bindingi909 – 9091ZincUniRule annotation
    Metal bindingi926 – 9261ZincUniRule annotation
    Metal bindingi929 – 9291ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciPPUT76869:GIXB-671-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:PputGB1_0649
    OrganismiPseudomonas putida (strain GB-1)
    Taxonomic identifieri76869 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
    ProteomesiUP000002157: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 943943Isoleucine--tRNA ligasePRO_1000088552Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi76869.PputGB1_0649.

    Structurei

    3D structure databases

    ProteinModelPortaliB0KM84.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi58 – 6811"HIGH" regionAdd
    BLAST
    Motifi608 – 6125"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiKPVHWCL.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B0KM84-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTDYKATLNL PDTAFPMKAG LPQREPQILQ RWDSIGLYQK LREIGKDRPK    50
    FVLHDGPPYA NGKIHIGHAL NKILKDMIVR SKTLAGFDAP YVPGWDCHGL 100
    PIEHKVEVTH GKHLSADRTR ELCREYAAEQ IEGQKTEFIR LGVLGDWDNP 150
    YKTMNFANEA GEIRALAEMV KQGFVFKGLK PVNWCFDCGS ALAEAEVEYA 200
    DKKSQTIDVA FPVADETKLA AAFGLASLAK PAAIVIWTTT PWTIPANQAL 250
    NIHPEFKYAL VDTGERLLVL AEELVESCLK RYNLEGSIIA TAQGSALELI 300
    NFRHPFYDRL SPIYLADYVE LGAGTGVVHS APAYGEDDFV TCKRYGMVND 350
    DILTPVQSNG VYVESLPFFG GQFIWKANPA IVEKLSEVGA LMHTETISHS 400
    YMHCWRHKTP LIYRATAQWF VGMDKQPTTG EPLRERALKA IEDTKFVPAW 450
    GQARLHSMIA NRPDWCISRQ RNWGVPIPFF LHKQTGELHP RTVELMEQVA 500
    KRVEQEGIEA WFKLDAAELL GVEADQYDKI TDTLDVWFDS GTTHWHVLRG 550
    SHDIGHTTGP VADLYLEGSD QHRGWFHSSL LTGCAIDNHA PYRELLTHGF 600
    TVDENGRKMS KSLGNTIEPE KVNNTLGADI LRLWVSATDY SGEMAVSEQI 650
    LQRSADAYRR IRNTARFLLS NLSGFDPARD LLAPEDMLAL DRWAVDRTLL 700
    LQRELEEHYS EYRFWNVYSK VHNFCVQELG GFYLDIIKDR QYTTGANSVA 750
    RRSCQTALYH ISEALVRWIA PILAFTADEI WQYLPGERNE SVMLNGWYQG 800
    LSELPEGTEL DRAYWDRVMA VKASVNKELE NQRTAKVIGG NLQAEVTLYA 850
    DEGLSADLGK LGDELRFVLI TSAASVVPFA QAPAEAVATE VEGLKLKVVK 900
    SGHAKCGRCW HFRADVGSHP EHPEICSRCV DNLSGSGEVR HYA 943
    Length:943
    Mass (Da):105,932
    Last modified:March 18, 2008 - v1
    Checksum:i4222E0116C510D3A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000926 Genomic DNA. Translation: ABY96560.1.
    RefSeqiYP_001666896.1. NC_010322.1.

    Genome annotation databases

    EnsemblBacteriaiABY96560; ABY96560; PputGB1_0649.
    GeneIDi5868400.
    KEGGippg:PputGB1_0649.
    PATRICi19928251. VBIPsePut76638_0654.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000926 Genomic DNA. Translation: ABY96560.1 .
    RefSeqi YP_001666896.1. NC_010322.1.

    3D structure databases

    ProteinModelPortali B0KM84.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 76869.PputGB1_0649.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABY96560 ; ABY96560 ; PputGB1_0649 .
    GeneIDi 5868400.
    KEGGi ppg:PputGB1_0649.
    PATRICi 19928251. VBIPsePut76638_0654.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi KPVHWCL.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci PPUT76869:GIXB-671-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: GB-1.

    Entry informationi

    Entry nameiSYI_PSEPG
    AccessioniPrimary (citable) accession number: B0KM84
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 20, 2008
    Last sequence update: March 18, 2008
    Last modified: October 1, 2014
    This is version 46 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3