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B0KM84

- SYI_PSEPG

UniProt

B0KM84 - SYI_PSEPG

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Pseudomonas putida (strain GB-1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei567 – 5671Aminoacyl-adenylateUniRule annotation
Binding sitei611 – 6111ATPUniRule annotation
Metal bindingi906 – 9061ZincUniRule annotation
Metal bindingi909 – 9091ZincUniRule annotation
Metal bindingi926 – 9261ZincUniRule annotation
Metal bindingi929 – 9291ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciPPUT76869:GIXB-671-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:PputGB1_0649
OrganismiPseudomonas putida (strain GB-1)
Taxonomic identifieri76869 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000002157: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 943943Isoleucine--tRNA ligasePRO_1000088552Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi76869.PputGB1_0649.

Structurei

3D structure databases

ProteinModelPortaliB0KM84.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionAdd
BLAST
Motifi608 – 6125"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B0KM84-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTDYKATLNL PDTAFPMKAG LPQREPQILQ RWDSIGLYQK LREIGKDRPK
60 70 80 90 100
FVLHDGPPYA NGKIHIGHAL NKILKDMIVR SKTLAGFDAP YVPGWDCHGL
110 120 130 140 150
PIEHKVEVTH GKHLSADRTR ELCREYAAEQ IEGQKTEFIR LGVLGDWDNP
160 170 180 190 200
YKTMNFANEA GEIRALAEMV KQGFVFKGLK PVNWCFDCGS ALAEAEVEYA
210 220 230 240 250
DKKSQTIDVA FPVADETKLA AAFGLASLAK PAAIVIWTTT PWTIPANQAL
260 270 280 290 300
NIHPEFKYAL VDTGERLLVL AEELVESCLK RYNLEGSIIA TAQGSALELI
310 320 330 340 350
NFRHPFYDRL SPIYLADYVE LGAGTGVVHS APAYGEDDFV TCKRYGMVND
360 370 380 390 400
DILTPVQSNG VYVESLPFFG GQFIWKANPA IVEKLSEVGA LMHTETISHS
410 420 430 440 450
YMHCWRHKTP LIYRATAQWF VGMDKQPTTG EPLRERALKA IEDTKFVPAW
460 470 480 490 500
GQARLHSMIA NRPDWCISRQ RNWGVPIPFF LHKQTGELHP RTVELMEQVA
510 520 530 540 550
KRVEQEGIEA WFKLDAAELL GVEADQYDKI TDTLDVWFDS GTTHWHVLRG
560 570 580 590 600
SHDIGHTTGP VADLYLEGSD QHRGWFHSSL LTGCAIDNHA PYRELLTHGF
610 620 630 640 650
TVDENGRKMS KSLGNTIEPE KVNNTLGADI LRLWVSATDY SGEMAVSEQI
660 670 680 690 700
LQRSADAYRR IRNTARFLLS NLSGFDPARD LLAPEDMLAL DRWAVDRTLL
710 720 730 740 750
LQRELEEHYS EYRFWNVYSK VHNFCVQELG GFYLDIIKDR QYTTGANSVA
760 770 780 790 800
RRSCQTALYH ISEALVRWIA PILAFTADEI WQYLPGERNE SVMLNGWYQG
810 820 830 840 850
LSELPEGTEL DRAYWDRVMA VKASVNKELE NQRTAKVIGG NLQAEVTLYA
860 870 880 890 900
DEGLSADLGK LGDELRFVLI TSAASVVPFA QAPAEAVATE VEGLKLKVVK
910 920 930 940
SGHAKCGRCW HFRADVGSHP EHPEICSRCV DNLSGSGEVR HYA
Length:943
Mass (Da):105,932
Last modified:March 18, 2008 - v1
Checksum:i4222E0116C510D3A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000926 Genomic DNA. Translation: ABY96560.1.
RefSeqiWP_012270369.1. NC_010322.1.
YP_001666896.1. NC_010322.1.

Genome annotation databases

EnsemblBacteriaiABY96560; ABY96560; PputGB1_0649.
GeneIDi5868400.
KEGGippg:PputGB1_0649.
PATRICi19928251. VBIPsePut76638_0654.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000926 Genomic DNA. Translation: ABY96560.1 .
RefSeqi WP_012270369.1. NC_010322.1.
YP_001666896.1. NC_010322.1.

3D structure databases

ProteinModelPortali B0KM84.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 76869.PputGB1_0649.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABY96560 ; ABY96560 ; PputGB1_0649 .
GeneIDi 5868400.
KEGGi ppg:PputGB1_0649.
PATRICi 19928251. VBIPsePut76638_0654.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci PPUT76869:GIXB-671-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: GB-1.

Entry informationi

Entry nameiSYI_PSEPG
AccessioniPrimary (citable) accession number: B0KM84
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: March 18, 2008
Last modified: October 29, 2014
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3