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Protein

Bifunctional protein FolD 2

Gene

folD2

Organism
Pseudomonas putida (strain GB-1)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the oxidation of 5,10-methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10-formyltetrahydrofolate.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + NADP+ = 5,10-methenyltetrahydrofolate + NADPH.UniRule annotation
5,10-methenyltetrahydrofolate + H2O = 10-formyltetrahydrofolate.UniRule annotation

Pathwayi: tetrahydrofolate interconversion

This protein is involved in the pathway tetrahydrofolate interconversion, which is part of One-carbon metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway tetrahydrofolate interconversion and in One-carbon metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei194NADPUniRule annotation1
Binding sitei235NADP; via amide nitrogenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi169 – 171NADPUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Multifunctional enzyme, Oxidoreductase
Biological processAmino-acid biosynthesis, Histidine biosynthesis, Methionine biosynthesis, One-carbon metabolism, Purine biosynthesis
LigandNADP

Enzyme and pathway databases

BioCyciPPUT76869:G1GAZ-2192-MONOMER
UniPathwayiUPA00193

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional protein FolD 2UniRule annotation
Including the following 2 domains:
Methylenetetrahydrofolate dehydrogenaseUniRule annotation (EC:1.5.1.5UniRule annotation)
Methenyltetrahydrofolate cyclohydrolaseUniRule annotation (EC:3.5.4.9UniRule annotation)
Gene namesi
Name:folD2UniRule annotation
Ordered Locus Names:PputGB1_2105
OrganismiPseudomonas putida (strain GB-1)
Taxonomic identifieri76869 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002157 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003405911 – 303Bifunctional protein FolD 2Add BLAST303

Proteomic databases

PRIDEiB0KLM9

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi76869.PputGB1_2105

Structurei

3D structure databases

ProteinModelPortaliB0KLM9
SMRiB0KLM9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CN0 Bacteria
COG0190 LUCA
HOGENOMiHOG000218242
KOiK01491
OMAiTRINAGR

Family and domain databases

HAMAPiMF_01576 THF_DHG_CYH, 1 hit
InterProiView protein in InterPro
IPR036291 NAD(P)-bd_dom_sf
IPR000672 THF_DH/CycHdrlase
IPR020630 THF_DH/CycHdrlase_cat_dom
IPR020867 THF_DH/CycHdrlase_CS
IPR020631 THF_DH/CycHdrlase_NAD-bd_dom
PANTHERiPTHR10025 PTHR10025, 1 hit
PfamiView protein in Pfam
PF00763 THF_DHG_CYH, 1 hit
PF02882 THF_DHG_CYH_C, 1 hit
PRINTSiPR00085 THFDHDRGNASE
SUPFAMiSSF51735 SSF51735, 1 hit
PROSITEiView protein in PROSITE
PS00767 THF_DHG_CYH_2, 1 hit

Sequencei

Sequence statusi: Complete.

B0KLM9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNALTSLKLI DGKATAARVL AEVREQVQEL RQGGVQPGLV VVLVGADAAS
60 70 80 90 100
QVYVRNKVLR AEEVGIRSQE HRLPADTTQA HLLTLIDRLN RDPEVNGILV
110 120 130 140 150
QLPLPAHIDE HCVLQAINPL KDVDGFHSEN VGGLAQGRDV LTPCTPSGCM
160 170 180 190 200
RLLRDACGEL RGKHAVVVGR SNIVGKPMAA LLLQADCTVT VVHSRSRDLP
210 220 230 240 250
ALCRQADILV AAVGKPRLIG ADCLKPGAVV IDVGINRINE DGQNHLVGDV
260 270 280 290 300
DFAAALPQVA GITPVPGGVG PMTIAYLMKN TLLALDLQQQ AAHQERTACL

SPC
Length:303
Mass (Da):32,148
Last modified:March 18, 2008 - v1
Checksum:i250DDB49D2466032
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000926 Genomic DNA Translation: ABY98006.1
RefSeqiWP_012271757.1, NC_010322.1

Genome annotation databases

EnsemblBacteriaiABY98006; ABY98006; PputGB1_2105
KEGGippg:PputGB1_2105

Similar proteinsi

Entry informationi

Entry nameiFOLD2_PSEPG
AccessioniPrimary (citable) accession number: B0KLM9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: March 18, 2008
Last modified: May 23, 2018
This is version 64 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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