Reviewed, UniProtKB/Swiss-Prot B0KLM9 (FOLD2_PSEPG)

Names and origin

Protein names

Recommended name:
    Bifunctional protein folD 2
Including the following 2 domains:
    1- Recommended name:
            Methylenetetrahydrofolate dehydrogenase
              EC=1.5.1.5
    2- Recommended name:
            Methenyltetrahydrofolate cyclohydrolase
              EC=3.5.4.9

Gene names

Name:	folD2
Ordered Locus Names:	PputGB1_2105

Organism

Pseudomonas putida (strain GB-1) [Complete proteome] [HAMAP]

Taxonomic identifier

76869 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	303 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the oxidation of 5,10-methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10-formyltetrahydrofolate By similarity.
Catalytic activity	5,10-methylenetetrahydrofolate + NADP+ = 5,10-methenyltetrahydrofolate + NADPH. HAMAP MF_01576 5,10-methenyltetrahydrofolate + H2O = 10-formyltetrahydrofolate. HAMAP MF_01576
Pathway	One-carbon metabolism; tetrahydrofolate pathway. HAMAP MF_01576
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Histidine biosynthesis Methionine biosynthesis One-carbon metabolism Purine biosynthesis
Ligand	NADP
Molecular function	Hydrolase Oxidoreductase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	folic acid and derivative biosynthetic process Inferred from electronic annotation. Source: InterPro histidine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW one-carbon compound metabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW purine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	binding Inferred from electronic annotation. Source: InterPro methenyltetrahydrofolate cyclohydrolase activity Inferred from electronic annotation. Source: HAMAP methylenetetrahydrofolate dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 303	303	Bifunctional protein folD 2 HAMAP MF_01576		PRO_0000340591

Sequences

	Sequence		Length	Mass (Da)
	B0KLM9-1 [UniParc]. Last modified March 18, 2008. Version 1. Checksum: 250DDB49D2466032	FASTA	303	32,148
	10 20 30 40 50 60 MNALTSLKLI DGKATAARVL AEVREQVQEL RQGGVQPGLV VVLVGADAAS QVYVRNKVLR 70 80 90 100 110 120 AEEVGIRSQE HRLPADTTQA HLLTLIDRLN RDPEVNGILV QLPLPAHIDE HCVLQAINPL 130 140 150 160 170 180 KDVDGFHSEN VGGLAQGRDV LTPCTPSGCM RLLRDACGEL RGKHAVVVGR SNIVGKPMAA 190 200 210 220 230 240 LLLQADCTVT VVHSRSRDLP ALCRQADILV AAVGKPRLIG ADCLKPGAVV IDVGINRINE 250 260 270 280 290 300 DGQNHLVGDV DFAAALPQVA GITPVPGGVG PMTIAYLMKN TLLALDLQQQ AAHQERTACL SPC

References

[1]

"Complete sequence of Pseudomonas putida GB-1." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Bruce D., Goodwin L., Chertkov O., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., McCarthy J.K., Richardson P. Submitted (JAN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000926 Genomic DNA. Translation: ABY98006.1.
RefSeq	YP_001668342.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5869894.
GenomeReviews	Gene locus PputGB1_2105 in contig CP000926_GR.
KEGG	ppg:PputGB1_2105.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	B0KLM9. MQIIDGK.
Family and domain databases
HAMAP	MF_01576. [Tree]
InterPro	IPR016040. NAD(P)-bd_dom. IPR000672. THF_DH/CycHdrlase. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00763. THF_DHG_CYH. 1 hit. PF02882. THF_DHG_CYH_C. 1 hit. [Graphical view]
PRINTS	PR00085. THFDHDRGNASE.
ProDom	PD002300. THFDhg/Cyc_hydro. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00766. THF_DHG_CYH_1. False negative. PS00767. THF_DHG_CYH_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FOLD2_PSEPG

Accession

Primary (citable) accession number: B0KLM9

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 10, 2008
Last sequence update:	March 18, 2008
Last modified:	June 16, 2009
This is version 9 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)