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Protein

Phosphoenolpyruvate carboxykinase [ATP]

Gene

pckA

Organism
Pseudomonas putida (strain GB-1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the gluconeogenesis. Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP) through direct phosphoryl transfer between the nucleoside triphosphate and OAA.UniRule annotation

Catalytic activityi

ATP + oxaloacetate = ADP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Mn2+UniRule annotationNote: Binds 1 Mn2+ ion per subunit.UniRule annotation

Pathwayi: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei45SubstrateUniRule annotation1
Binding sitei179SubstrateUniRule annotation1
Metal bindingi185ManganeseUniRule annotation1
Binding sitei185ATPUniRule annotation1
Binding sitei185SubstrateUniRule annotation1
Metal bindingi204Manganese; via tele nitrogenUniRule annotation1
Binding sitei204ATPUniRule annotation1
Metal bindingi241ManganeseUniRule annotation1
Binding sitei269ATPUniRule annotation1
Binding sitei305ATPUniRule annotation1
Binding sitei305SubstrateUniRule annotation1
Binding sitei431ATPUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi220 – 228ATPUniRule annotation9

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Gluconeogenesis

Keywords - Ligandi

ATP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [ATP]UniRule annotation (EC:4.1.1.49UniRule annotation)
Short name:
PCKUniRule annotation
Short name:
PEP carboxykinaseUniRule annotation
Short name:
PEPCKUniRule annotation
Gene namesi
Name:pckAUniRule annotation
Ordered Locus Names:PputGB1_0278
OrganismiPseudomonas putida (strain GB-1)
Taxonomic identifieri76869 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002157 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000809981 – 513Phosphoenolpyruvate carboxykinase [ATP]Add BLAST513

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi76869.PputGB1_0278.

Structurei

3D structure databases

ProteinModelPortaliB0KI05.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the phosphoenolpyruvate carboxykinase [ATP] family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DJ1. Bacteria.
COG1866. LUCA.
HOGENOMiHOG000271471.
KOiK01610.
OMAiRFIVKEP.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00453. PEPCK_ATP. 1 hit.
InterProiIPR001272. PEP_carboxykinase_ATP.
IPR013035. PEP_carboxykinase_C.
IPR008210. PEP_carboxykinase_N.
IPR015994. PEPCK_ATP_CS.
[Graphical view]
PfamiPF01293. PEPCK_ATP. 1 hit.
[Graphical view]
PIRSFiPIRSF006294. PEP_crbxkin. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
TIGRFAMsiTIGR00224. pckA. 1 hit.
PROSITEiPS00532. PEPCK_ATP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B0KI05-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTQANNTVYT DLSVDELVKE ALQRGEGVLA DTGALVVETG HRTGRSPADR
60 70 80 90 100
FIVEEPSTQD AISWGPINRK FPADKFDALW ARVEAFNNAQ DHFVSYVHVG
110 120 130 140 150
AAAEHYLPVK MTTQTAWQNL FGRCLFINPE QFNPAGRDQW QVLNVANFVC
160 170 180 190 200
EPERDGTNSD GCVIINFAQK KVLIAGMRYA GEMKKAMFSV QNFLLPAADV
210 220 230 240 250
LPMHCAANIG EEGDVTLFFG LSGTGKTTLS ADESRYLIGD DEHGWGEGVV
260 270 280 290 300
FNMEGGCYAK CIDLSEKNEP VIWKAIKHGA VLENVVLDAN KHADYTDVSL
310 320 330 340 350
TQNSRAAYPL EHVAKRSEAN LGGEPNAVIF LTCDLTGVLP PVSILNNEQA
360 370 380 390 400
AYHFLSGYTA LVGSTEMGSG GGIKSTFSTC FGAPFFPRPA GEYAELLIKR
410 420 430 440 450
INGFNSKVYL VNTGWTGGGY GVGKRFSIPT TRAVIAAIQS GALVGAETEH
460 470 480 490 500
LDIINLDVPK AVPGVETELL NPRTNWADKA AYDEAAKGLA KLFIENFKKF
510
EVSDAIKAAG PQL
Length:513
Mass (Da):55,569
Last modified:March 18, 2008 - v1
Checksum:i94A3A11995244152
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000926 Genomic DNA. Translation: ABY96191.1.
RefSeqiWP_012270058.1. NC_010322.1.

Genome annotation databases

EnsemblBacteriaiABY96191; ABY96191; PputGB1_0278.
KEGGippg:PputGB1_0278.
PATRICi19927491. VBIPsePut76638_0289.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000926 Genomic DNA. Translation: ABY96191.1.
RefSeqiWP_012270058.1. NC_010322.1.

3D structure databases

ProteinModelPortaliB0KI05.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi76869.PputGB1_0278.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABY96191; ABY96191; PputGB1_0278.
KEGGippg:PputGB1_0278.
PATRICi19927491. VBIPsePut76638_0289.

Phylogenomic databases

eggNOGiENOG4105DJ1. Bacteria.
COG1866. LUCA.
HOGENOMiHOG000271471.
KOiK01610.
OMAiRFIVKEP.

Enzyme and pathway databases

UniPathwayiUPA00138.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00453. PEPCK_ATP. 1 hit.
InterProiIPR001272. PEP_carboxykinase_ATP.
IPR013035. PEP_carboxykinase_C.
IPR008210. PEP_carboxykinase_N.
IPR015994. PEPCK_ATP_CS.
[Graphical view]
PfamiPF01293. PEPCK_ATP. 1 hit.
[Graphical view]
PIRSFiPIRSF006294. PEP_crbxkin. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
TIGRFAMsiTIGR00224. pckA. 1 hit.
PROSITEiPS00532. PEPCK_ATP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPCKA_PSEPG
AccessioniPrimary (citable) accession number: B0KI05
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: March 18, 2008
Last modified: November 2, 2016
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.