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Protein

Probable alpha-L-glutamate ligase

Gene

rimK

Organism
Pseudomonas putida (strain GB-1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Cofactori

Mg2+UniRule annotation, Mn2+UniRule annotationNote: Binds 2 magnesium or manganese ions per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei141 – 1411ATPUniRule annotation
Binding sitei187 – 1871ATPUniRule annotation
Metal bindingi248 – 2481Magnesium or manganese 1UniRule annotation
Metal bindingi260 – 2601Magnesium or manganese 1UniRule annotation
Metal bindingi260 – 2601Magnesium or manganese 2UniRule annotation
Metal bindingi262 – 2621Magnesium or manganese 2UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi178 – 1792ATPUniRule annotation
Nucleotide bindingi211 – 2133ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciPPUT76869:GIXB-282-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable alpha-L-glutamate ligaseUniRule annotation (EC:6.3.2.-UniRule annotation)
Gene namesi
Name:rimKUniRule annotation
Ordered Locus Names:PputGB1_0273
OrganismiPseudomonas putida (strain GB-1)
Taxonomic identifieri76869 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000002157 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 301301Probable alpha-L-glutamate ligasePRO_1000087749Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi76869.PputGB1_0273.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini104 – 287184ATP-graspUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the RimK family.UniRule annotation
Contains 1 ATP-grasp domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG0189.
HOGENOMiHOG000293092.
KOiK05844.
OMAiCYMNITS.
OrthoDBiEOG6DZDX8.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPiMF_01552. RimK.
InterProiIPR011761. ATP-grasp.
IPR013651. ATP-grasp_RimK-type.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR023533. RimK.
IPR004666. RpS6_RimK/Lys_biosynth_LsyX.
[Graphical view]
PfamiPF08443. RimK. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00768. rimK_fam. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B0KI00-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKIAVLSRNP RLYSTRRLVE AGIQRGHEMV VIDTLRAYMN IASHKPQIHY
60 70 80 90 100
RGKPLEGFDA VIPRIGASVT FYGCAVLRQF EMMGVYPLNE SVAIARSRDK
110 120 130 140 150
LRSLQLLSRR GIGLPITGFA HSPDDIPDLI QMVNGAPLVI KVLEGTQGIG
160 170 180 190 200
VVLCETTQAA ESVIEAFMGL KQNIMVQEYI KEAGGADIRC FVVGDKVIAS
210 220 230 240 250
MKRQAKPGEF RSNLHRGGVA SLIKITPEER ITAIRAAKVM GLSVAGVDIL
260 270 280 290 300
RSNHGPLVME VNSSPGLEGI EVTTGKNVAG MIIEHLEKNG GPNQTRTKGK

G
Length:301
Mass (Da):32,645
Last modified:March 18, 2008 - v1
Checksum:iEA1DC324F0FE6B8E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000926 Genomic DNA. Translation: ABY96186.1.
RefSeqiWP_008099005.1. NC_010322.1.
YP_001666522.1. NC_010322.1.

Genome annotation databases

EnsemblBacteriaiABY96186; ABY96186; PputGB1_0273.
KEGGippg:PputGB1_0273.
PATRICi19927481. VBIPsePut76638_0284.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000926 Genomic DNA. Translation: ABY96186.1.
RefSeqiWP_008099005.1. NC_010322.1.
YP_001666522.1. NC_010322.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi76869.PputGB1_0273.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABY96186; ABY96186; PputGB1_0273.
KEGGippg:PputGB1_0273.
PATRICi19927481. VBIPsePut76638_0284.

Phylogenomic databases

eggNOGiCOG0189.
HOGENOMiHOG000293092.
KOiK05844.
OMAiCYMNITS.
OrthoDBiEOG6DZDX8.

Enzyme and pathway databases

BioCyciPPUT76869:GIXB-282-MONOMER.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPiMF_01552. RimK.
InterProiIPR011761. ATP-grasp.
IPR013651. ATP-grasp_RimK-type.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR023533. RimK.
IPR004666. RpS6_RimK/Lys_biosynth_LsyX.
[Graphical view]
PfamiPF08443. RimK. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00768. rimK_fam. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: GB-1.

Entry informationi

Entry nameiRIMK_PSEPG
AccessioniPrimary (citable) accession number: B0KI00
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: March 18, 2008
Last modified: May 27, 2015
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.