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B0KI00

- RIMK_PSEPG

UniProt

B0KI00 - RIMK_PSEPG

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Protein

Probable alpha-L-glutamate ligase

Gene
rimK, PputGB1_0273
Organism
Pseudomonas putida (strain GB-1)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Cofactori

Binds 2 magnesium or manganese ions per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei141 – 1411ATP By similarity
Binding sitei187 – 1871ATP By similarity
Metal bindingi248 – 2481Magnesium or manganese 1 By similarity
Metal bindingi260 – 2601Magnesium or manganese 1 By similarity
Metal bindingi260 – 2601Magnesium or manganese 2 By similarity
Metal bindingi262 – 2621Magnesium or manganese 2 By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi178 – 1792ATP By similarity
Nucleotide bindingi211 – 2133ATP By similarity

GO - Molecular functioni

  1. acid-amino acid ligase activity Source: UniProtKB-HAMAP
  2. ATP binding Source: UniProtKB-HAMAP
  3. magnesium ion binding Source: UniProtKB-HAMAP
  4. manganese ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. cellular protein modification process Source: InterPro
  2. translation Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciPPUT76869:GIXB-282-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable alpha-L-glutamate ligase (EC:6.3.2.-)
Gene namesi
Name:rimK
Ordered Locus Names:PputGB1_0273
OrganismiPseudomonas putida (strain GB-1)
Taxonomic identifieri76869 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000002157: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 301301Probable alpha-L-glutamate ligaseUniRule annotationPRO_1000087749Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi76869.PputGB1_0273.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini104 – 287184ATP-graspAdd
BLAST

Sequence similaritiesi

Belongs to the RimK family.
Contains 1 ATP-grasp domain.

Phylogenomic databases

eggNOGiCOG0189.
HOGENOMiHOG000293092.
KOiK05844.
OMAiCREPRLY.
OrthoDBiEOG6DZDX8.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPiMF_01552. RimK.
InterProiIPR011761. ATP-grasp.
IPR013651. ATP-grasp_RimK-type.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR023533. Ribosomal_S6_modifier_RimK.
IPR004666. RpS6_RimK/Lys_biosynth_LsyX.
[Graphical view]
PfamiPF08443. RimK. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00768. rimK_fam. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B0KI00-1 [UniParc]FASTAAdd to Basket

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MKIAVLSRNP RLYSTRRLVE AGIQRGHEMV VIDTLRAYMN IASHKPQIHY    50
RGKPLEGFDA VIPRIGASVT FYGCAVLRQF EMMGVYPLNE SVAIARSRDK 100
LRSLQLLSRR GIGLPITGFA HSPDDIPDLI QMVNGAPLVI KVLEGTQGIG 150
VVLCETTQAA ESVIEAFMGL KQNIMVQEYI KEAGGADIRC FVVGDKVIAS 200
MKRQAKPGEF RSNLHRGGVA SLIKITPEER ITAIRAAKVM GLSVAGVDIL 250
RSNHGPLVME VNSSPGLEGI EVTTGKNVAG MIIEHLEKNG GPNQTRTKGK 300
G 301
Length:301
Mass (Da):32,645
Last modified:March 18, 2008 - v1
Checksum:iEA1DC324F0FE6B8E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000926 Genomic DNA. Translation: ABY96186.1.
RefSeqiYP_001666522.1. NC_010322.1.

Genome annotation databases

EnsemblBacteriaiABY96186; ABY96186; PputGB1_0273.
GeneIDi5867970.
KEGGippg:PputGB1_0273.
PATRICi19927481. VBIPsePut76638_0284.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000926 Genomic DNA. Translation: ABY96186.1 .
RefSeqi YP_001666522.1. NC_010322.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 76869.PputGB1_0273.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABY96186 ; ABY96186 ; PputGB1_0273 .
GeneIDi 5867970.
KEGGi ppg:PputGB1_0273.
PATRICi 19927481. VBIPsePut76638_0284.

Phylogenomic databases

eggNOGi COG0189.
HOGENOMi HOG000293092.
KOi K05844.
OMAi CREPRLY.
OrthoDBi EOG6DZDX8.

Enzyme and pathway databases

BioCyci PPUT76869:GIXB-282-MONOMER.

Family and domain databases

Gene3Di 3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPi MF_01552. RimK.
InterProi IPR011761. ATP-grasp.
IPR013651. ATP-grasp_RimK-type.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR023533. Ribosomal_S6_modifier_RimK.
IPR004666. RpS6_RimK/Lys_biosynth_LsyX.
[Graphical view ]
Pfami PF08443. RimK. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00768. rimK_fam. 1 hit.
PROSITEi PS50975. ATP_GRASP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: GB-1.

Entry informationi

Entry nameiRIMK_PSEPG
AccessioniPrimary (citable) accession number: B0KI00
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: March 18, 2008
Last modified: May 14, 2014
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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