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Protein

Translation initiation factor IF-2

Gene

infB

Organism
Pseudomonas putida (strain GB-1)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex.UniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi351 – 358GTPUniRule annotation8
Nucleotide bindingi397 – 401GTPUniRule annotation5
Nucleotide bindingi451 – 454GTPUniRule annotation4

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Initiation factor

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Translation initiation factor IF-2UniRule annotation
Gene namesi
Name:infBUniRule annotation
Ordered Locus Names:PputGB1_4712
OrganismiPseudomonas putida (strain GB-1)
Taxonomic identifieri76869 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002157 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000756141 – 842Translation initiation factor IF-2Add BLAST842

Interactioni

Protein-protein interaction databases

STRINGi76869.PputGB1_4712.

Structurei

3D structure databases

ProteinModelPortaliB0KHX8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini342 – 509tr-type GAdd BLAST168

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni351 – 358G1By similarity8
Regioni376 – 380G2By similarity5
Regioni397 – 400G3By similarity4
Regioni451 – 454G4By similarity4
Regioni487 – 489G5By similarity3

Sequence similaritiesi

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. IF-2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107QHU. Bacteria.
COG0532. LUCA.
HOGENOMiHOG000076907.
KOiK02519.
OMAiEMGHTAK.

Family and domain databases

Gene3Di3.40.50.10050. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00100_B. IF_2_B. 1 hit.
InterProiIPR009061. DNA-bd_dom_put.
IPR013575. IF2_assoc_dom_bac.
IPR006847. IF2_N.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
IPR000178. TF_IF2_bacterial-like.
IPR023115. TIF_IF2_dom3.
IPR009000. Transl_B-barrel.
[Graphical view]
PfamiPF11987. IF-2. 1 hit.
PF08364. IF2_assoc. 1 hit.
PF04760. IF2_N. 2 hits.
[Graphical view]
SUPFAMiSSF46955. SSF46955. 1 hit.
SSF50447. SSF50447. 2 hits.
SSF52156. SSF52156. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00487. IF-2. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS51722. G_TR_2. 1 hit.
PS01176. IF2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B0KHX8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTQVTVKELA QEVEAPVERL LQQMREAGLP HTDAGQVVTD NEKQTLLTHL
60 70 80 90 100
KSSHKSKAEE PRKITLQRKT TSTLRVAGSK SISVEVRKKK VFVQRSPEEI
110 120 130 140 150
QAEQKRELDE RRAAENAARD KVEAEVRQRN EEQARRQAAD SAVAAPAPAA
160 170 180 190 200
KPEPAPAAAP APVVADAPAS EDAAARAAER KKDETRRNES RTRDDDRRRG
210 220 230 240 250
EAPRVSIKVK VKEKEKAPTP RAAPRTTDEE SDGARRGRGG KGKLKKRNQH
260 270 280 290 300
GFQNPTGPVI RDVTIGETIT VSELANQMSV KGAEVVKFMF KMGTPVTINQ
310 320 330 340 350
VLDQETAQLI AEELGHKVTL VSDTALEDSL AESLKFEGQT ESRAPVVTVM
360 370 380 390 400
GHVDHGKTSL LDYIRRAKVA AGEAGGITQH IGAYHVETDR GMVTFLDTPG
410 420 430 440 450
HAAFTQMRAR GAKATDIVIL VVAADDGVMP QTREAVQHAK AAGVPLVVAV
460 470 480 490 500
NKIDKPGADL DRIRNELSVE GVTSEDWGGD TPFVKVSAKM GTGVDELLEA
510 520 530 540 550
VLLQAEILEL TATPTAPGRG VVVESRLDKG RGPVATILVQ DGTLRQGDMV
560 570 580 590 600
LCGSNYGRVR AMLDENGKPV KEAGPSIPVE ILGLDGTPEA GDELSVVADE
610 620 630 640 650
KKAREVALFR QGKYREVKLA RAHAGKLENI FETMGQEEKK TLNIVLKTDV
660 670 680 690 700
RGSLEALQGS LGGLGNDEVQ VRVIGGGVGG ITESDANLAL ASNAVLFGFN
710 720 730 740 750
VRADAGARKI VEQEGLDMRY YNVIYDIIED VKKALTGMLG SDVRENILGV
760 770 780 790 800
AEVRDVFRSP KFGAIAGCMV IEGTVYRNRP IRVLRDDVVI FEGELESLRR
810 820 830 840
FKDDASEVRS GMECGIGVKS YNDVKVGDKI EVFEKVQVAR TL
Length:842
Mass (Da):91,179
Last modified:March 18, 2008 - v1
Checksum:i22906692EB02EE55
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000926 Genomic DNA. Translation: ABZ00599.1.
RefSeqiWP_012274244.1. NC_010322.1.

Genome annotation databases

EnsemblBacteriaiABZ00599; ABZ00599; PputGB1_4712.
KEGGippg:PputGB1_4712.
PATRICi19936515. VBIPsePut76638_4719.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000926 Genomic DNA. Translation: ABZ00599.1.
RefSeqiWP_012274244.1. NC_010322.1.

3D structure databases

ProteinModelPortaliB0KHX8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi76869.PputGB1_4712.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABZ00599; ABZ00599; PputGB1_4712.
KEGGippg:PputGB1_4712.
PATRICi19936515. VBIPsePut76638_4719.

Phylogenomic databases

eggNOGiENOG4107QHU. Bacteria.
COG0532. LUCA.
HOGENOMiHOG000076907.
KOiK02519.
OMAiEMGHTAK.

Family and domain databases

Gene3Di3.40.50.10050. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00100_B. IF_2_B. 1 hit.
InterProiIPR009061. DNA-bd_dom_put.
IPR013575. IF2_assoc_dom_bac.
IPR006847. IF2_N.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
IPR000178. TF_IF2_bacterial-like.
IPR023115. TIF_IF2_dom3.
IPR009000. Transl_B-barrel.
[Graphical view]
PfamiPF11987. IF-2. 1 hit.
PF08364. IF2_assoc. 1 hit.
PF04760. IF2_N. 2 hits.
[Graphical view]
SUPFAMiSSF46955. SSF46955. 1 hit.
SSF50447. SSF50447. 2 hits.
SSF52156. SSF52156. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00487. IF-2. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS51722. G_TR_2. 1 hit.
PS01176. IF2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiIF2_PSEPG
AccessioniPrimary (citable) accession number: B0KHX8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: March 18, 2008
Last modified: November 2, 2016
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.