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B0KHX8 (IF2_PSEPG) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 45. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Translation initiation factor IF-2
Gene names
Name:infB
Ordered Locus Names:PputGB1_4712
OrganismPseudomonas putida (strain GB-1) [Complete proteome] [HAMAP]
Taxonomic identifier76869 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Protein attributes

Sequence length842 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex By similarity. HAMAP-Rule MF_00100

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00100.

Sequence similarities

Belongs to the IF-2 family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandGTP-binding
Nucleotide-binding
   Molecular functionInitiation factor
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionGTP binding

Inferred from electronic annotation. Source: UniProtKB-KW

GTPase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

translation initiation factor activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 842842Translation initiation factor IF-2 HAMAP-Rule MF_00100
PRO_1000075614

Regions

Nucleotide binding351 – 3588GTP By similarity
Nucleotide binding397 – 4015GTP By similarity
Nucleotide binding451 – 4544GTP By similarity
Region345 – 493149G-domain HAMAP-Rule MF_00100

Sequences

Sequence LengthMass (Da)Tools
B0KHX8 [UniParc].

Last modified March 18, 2008. Version 1.
Checksum: 22906692EB02EE55

FASTA84291,179
        10         20         30         40         50         60 
MTQVTVKELA QEVEAPVERL LQQMREAGLP HTDAGQVVTD NEKQTLLTHL KSSHKSKAEE 

        70         80         90        100        110        120 
PRKITLQRKT TSTLRVAGSK SISVEVRKKK VFVQRSPEEI QAEQKRELDE RRAAENAARD 

       130        140        150        160        170        180 
KVEAEVRQRN EEQARRQAAD SAVAAPAPAA KPEPAPAAAP APVVADAPAS EDAAARAAER 

       190        200        210        220        230        240 
KKDETRRNES RTRDDDRRRG EAPRVSIKVK VKEKEKAPTP RAAPRTTDEE SDGARRGRGG 

       250        260        270        280        290        300 
KGKLKKRNQH GFQNPTGPVI RDVTIGETIT VSELANQMSV KGAEVVKFMF KMGTPVTINQ 

       310        320        330        340        350        360 
VLDQETAQLI AEELGHKVTL VSDTALEDSL AESLKFEGQT ESRAPVVTVM GHVDHGKTSL 

       370        380        390        400        410        420 
LDYIRRAKVA AGEAGGITQH IGAYHVETDR GMVTFLDTPG HAAFTQMRAR GAKATDIVIL 

       430        440        450        460        470        480 
VVAADDGVMP QTREAVQHAK AAGVPLVVAV NKIDKPGADL DRIRNELSVE GVTSEDWGGD 

       490        500        510        520        530        540 
TPFVKVSAKM GTGVDELLEA VLLQAEILEL TATPTAPGRG VVVESRLDKG RGPVATILVQ 

       550        560        570        580        590        600 
DGTLRQGDMV LCGSNYGRVR AMLDENGKPV KEAGPSIPVE ILGLDGTPEA GDELSVVADE 

       610        620        630        640        650        660 
KKAREVALFR QGKYREVKLA RAHAGKLENI FETMGQEEKK TLNIVLKTDV RGSLEALQGS 

       670        680        690        700        710        720 
LGGLGNDEVQ VRVIGGGVGG ITESDANLAL ASNAVLFGFN VRADAGARKI VEQEGLDMRY 

       730        740        750        760        770        780 
YNVIYDIIED VKKALTGMLG SDVRENILGV AEVRDVFRSP KFGAIAGCMV IEGTVYRNRP 

       790        800        810        820        830        840 
IRVLRDDVVI FEGELESLRR FKDDASEVRS GMECGIGVKS YNDVKVGDKI EVFEKVQVAR 


TL 

« Hide

References

[1]"Complete sequence of Pseudomonas putida GB-1."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Bruce D., Goodwin L., Chertkov O., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M. expand/collapse author list , Hauser L., Kyrpides N., Kim E., McCarthy J.K., Richardson P.
Submitted (JAN-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: GB-1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000926 Genomic DNA. Translation: ABZ00599.1.
RefSeqYP_001670934.1. NC_010322.1.

3D structure databases

ProteinModelPortalB0KHX8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING76869.PputGB1_4712.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABZ00599; ABZ00599; PputGB1_4712.
GeneID5872523.
KEGGppg:PputGB1_4712.
PATRIC19936515. VBIPsePut76638_4719.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0532.
HOGENOMHOG000076907.
KOK02519.
OMAIKFMFKM.
OrthoDBEOG67HJSV.
ProtClustDBPRK05306.

Enzyme and pathway databases

BioCycPPUT76869:GIXB-4793-MONOMER.

Family and domain databases

Gene3D3.40.50.10050. 1 hit.
3.40.50.300. 1 hit.
HAMAPMF_00100_B. IF_2_B.
InterProIPR009061. DNA-bd_dom_put.
IPR000795. EF_GTP-bd_dom.
IPR013575. IF2_assoc_dom_bac.
IPR006847. IF2_N.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR000178. TF_IF2_bacterial-like.
IPR015760. TIF_IF2.
IPR023115. TIF_IF2_dom3.
IPR009000. Transl_B-barrel.
IPR004161. Transl_elong_EFTu/EF1A_2.
[Graphical view]
PANTHERPTHR23115:SF41. PTHR23115:SF41. 1 hit.
PfamPF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF11987. IF-2. 1 hit.
PF08364. IF2_assoc. 1 hit.
PF04760. IF2_N. 2 hits.
[Graphical view]
SUPFAMSSF46955. SSF46955. 1 hit.
SSF50447. SSF50447. 2 hits.
SSF52156. SSF52156. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsTIGR00487. IF-2. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEPS01176. IF2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameIF2_PSEPG
AccessionPrimary (citable) accession number: B0KHX8
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: March 18, 2008
Last modified: April 16, 2014
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Translation initiation factors

List of translation initiation factor entries