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B0KHW7

- B0KHW7_PSEPG

UniProt

B0KHW7 - B0KHW7_PSEPG

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Protein

Acetyl-coenzyme A synthetase

Gene

acsA

Organism
Pseudomonas putida (strain GB-1)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the conversion of acetate into acetyl-CoA (AcCoA), an essential intermediate at the junction of anabolic and catabolic pathways. AcsA undergoes a two-step reaction. In the first half reaction, AcsA combines acetate with ATP to form acetyl-adenylate (AcAMP) intermediate. In the second half reaction, it can then transfer the acetyl group from AcAMP to the sulfhydryl group of CoA, forming the product AcCoA.UniRule annotation

Catalytic activityi

ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA.UniRule annotation

Cofactori

Magnesium.UniRule annotationSAAS annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei307 – 3071Coenzyme AUniRule annotation
Binding sitei331 – 3311Coenzyme AUniRule annotation
Binding sitei496 – 4961ATPUniRule annotation
Binding sitei511 – 5111ATPUniRule annotation
Binding sitei519 – 5191Coenzyme A; via carbonyl oxygenUniRule annotation
Binding sitei522 – 5221ATPUniRule annotation
Metal bindingi533 – 5331Magnesium; via carbonyl oxygenUniRule annotation
Metal bindingi535 – 5351Magnesium; via carbonyl oxygenUniRule annotation
Metal bindingi538 – 5381Magnesium; via carbonyl oxygenUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi383 – 3853ATPUniRule annotation
Nucleotide bindingi407 – 4126ATPUniRule annotation

GO - Molecular functioni

  1. acetate-CoA ligase activity Source: UniProtKB-HAMAP
  2. AMP binding Source: InterPro
  3. ATP binding Source: UniProtKB-KW
  4. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. acetyl-CoA biosynthetic process from acetate Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

LigaseUniRule annotationImported

Keywords - Ligandi

ATP-bindingUniRule annotation, MagnesiumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciPPUT76869:GIXB-4782-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Acetyl-coenzyme A synthetaseUniRule annotation (EC:6.2.1.1UniRule annotation)
Short name:
AcCoA synthetaseUniRule annotation
Short name:
AcsUniRule annotation
Alternative name(s):
Acetate--CoA ligaseUniRule annotation
Acyl-activating enzymeUniRule annotation
Gene namesi
Name:acsAUniRule annotation
Ordered Locus Names:PputGB1_4701Imported
OrganismiPseudomonas putida (strain GB-1)Imported
Taxonomic identifieri76869 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000002157: Chromosome

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei605 – 6051N6-acetyllysineUniRule annotation

Post-translational modificationi

Acetylated. Deacetylation by the SIR2-homolog deacetylase activates the enzyme.UniRule annotation

Keywords - PTMi

AcetylationUniRule annotation

Interactioni

Protein-protein interaction databases

STRINGi76869.PputGB1_4701.

Structurei

3D structure databases

ProteinModelPortaliB0KHW7.
SMRiB0KHW7. Positions 21-641.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni189 – 1924Coenzyme A bindingUniRule annotation

Sequence similaritiesi

Belongs to the ATP-dependent AMP-binding enzyme family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0365.
HOGENOMiHOG000229981.
KOiK01895.
OMAiRRIFEPT.
OrthoDBiEOG68WR2H.

Family and domain databases

HAMAPiMF_01123. Ac_CoA_synth.
InterProiIPR011904. Ac_CoA_lig.
IPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view]
PfamiPF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02188. Ac_CoA_lig_AcsA. 1 hit.
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B0KHW7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MFDIRNYPQA LAVSQSATPT PDEYHRLYRQ SVDDPDTFWA EQAKRLDWIK
60 70 80 90 100
PWSSVQQCDL NTGKARWFDG AQLNVSYNCI DRHLAQRGEQ TALLWEGDDP
110 120 130 140 150
KDSKAITYRE LHRQVCRLAN AMKARGVKKG DRVCIYMPMI PEAAFAMLAC
160 170 180 190 200
TRIGAIHSVV FGGFSPDALR DRILDADCRT VITADEGVRG GKRVPLKQNV
210 220 230 240 250
DKALASCPAV SSVLVVRRTG GDVAWAEGRD LWYHEVTEKA GDDCPPEPMA
260 270 280 290 300
AEDPLFILYT SGSTGKPKGV LHTTGGYLLQ ATMTFKVVFD YRDGEVFWCT
310 320 330 340 350
ADVGWVTGHS YIVYGPLANG AISLMFEGVP NYPDTSRFWQ VVDKHQVNIF
360 370 380 390 400
YTAPTALRAL MREGSAPLQG TSRKSLRLLG SVGEPINPEA WEWYFEAVGQ
410 420 430 440 450
KRCPIVDTWW QTETGGIMLT PLPGSQALKP GCATQPMFGV QPALLDEKGK
460 470 480 490 500
LIEGPGAGLL VIKASWPGQI RSVYGDHQRM VDTYFKPMPG YYFTGDGARR
510 520 530 540 550
DADGDYWITG RIDDVINVSG HRIGTAEVES ALVLHDSVAE AAVVGYPHDL
560 570 580 590 600
KGQGVYAFVT TMNGVTPDDT LKAELLALVS KEIGSFAKPE LIQWAPALPK
610 620 630 640
TRSGKIMRRI LRKIACNELD NLGDTSTLAD PSVVQGLIDK RLNQ
Length:644
Mass (Da):71,051
Last modified:March 18, 2008 - v1
Checksum:i6DA55322F09A7A93
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000926 Genomic DNA. Translation: ABZ00588.1.
RefSeqiWP_012274233.1. NC_010322.1.
YP_001670923.1. NC_010322.1.

Genome annotation databases

EnsemblBacteriaiABZ00588; ABZ00588; PputGB1_4701.
GeneIDi5872512.
KEGGippg:PputGB1_4701.
PATRICi19936495. VBIPsePut76638_4709.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000926 Genomic DNA. Translation: ABZ00588.1 .
RefSeqi WP_012274233.1. NC_010322.1.
YP_001670923.1. NC_010322.1.

3D structure databases

ProteinModelPortali B0KHW7.
SMRi B0KHW7. Positions 21-641.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 76869.PputGB1_4701.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABZ00588 ; ABZ00588 ; PputGB1_4701 .
GeneIDi 5872512.
KEGGi ppg:PputGB1_4701.
PATRICi 19936495. VBIPsePut76638_4709.

Phylogenomic databases

eggNOGi COG0365.
HOGENOMi HOG000229981.
KOi K01895.
OMAi RRIFEPT.
OrthoDBi EOG68WR2H.

Enzyme and pathway databases

BioCyci PPUT76869:GIXB-4782-MONOMER.

Family and domain databases

HAMAPi MF_01123. Ac_CoA_synth.
InterProi IPR011904. Ac_CoA_lig.
IPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view ]
Pfami PF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR02188. Ac_CoA_lig_AcsA. 1 hit.
PROSITEi PS00455. AMP_BINDING. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: GB-1Imported.

Entry informationi

Entry nameiB0KHW7_PSEPG
AccessioniPrimary (citable) accession number: B0KHW7
Entry historyi
Integrated into UniProtKB/TrEMBL: March 18, 2008
Last sequence update: March 18, 2008
Last modified: October 29, 2014
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

External Data

Dasty 3