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B0KHW7

- B0KHW7_PSEPG

UniProt

B0KHW7 - B0KHW7_PSEPG

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Protein

Acetyl-coenzyme A synthetase

Gene
acsA, PputGB1_4701
Organism
Pseudomonas putida (strain GB-1)
Status
Unreviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of acetate into acetyl-CoA (AcCoA), an essential intermediate at the junction of anabolic and catabolic pathways. AcsA undergoes a two-step reaction. In the first half reaction, AcsA combines acetate with ATP to form acetyl-adenylate (AcAMP) intermediate. In the second half reaction, it can then transfer the acetyl group from AcAMP to the sulfhydryl group of CoA, forming the product AcCoA By similarity.UniRule annotation

Catalytic activityi

ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA.UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotationSAAS annotations

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei307 – 3071Coenzyme A By similarityUniRule annotation
Binding sitei331 – 3311Coenzyme A By similarityUniRule annotation
Binding sitei383 – 3831Substrate; via nitrogen amide By similarityUniRule annotation
Binding sitei496 – 4961Substrate By similarityUniRule annotation
Binding sitei511 – 5111Substrate By similarityUniRule annotation
Active sitei513 – 5131 By similarityUniRule annotation
Binding sitei519 – 5191Coenzyme A By similarityUniRule annotation
Binding sitei522 – 5221Substrate By similarityUniRule annotation
Metal bindingi533 – 5331Magnesium; via carbonyl oxygen By similarityUniRule annotation
Metal bindingi535 – 5351Magnesium; via carbonyl oxygen By similarityUniRule annotation
Metal bindingi538 – 5381Magnesium; via carbonyl oxygen By similarityUniRule annotation
Binding sitei580 – 5801Coenzyme A By similarityUniRule annotation

GO - Molecular functioni

  1. acetate-CoA ligase activity Source: UniProtKB-HAMAP
  2. AMP binding Source: InterPro
  3. ATP binding Source: UniProtKB-KW
  4. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. acetyl-CoA biosynthetic process from acetate Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

LigaseUniRule annotationImported

Keywords - Ligandi

ATP-bindingUniRule annotation, MagnesiumUniRule annotationSAAS annotations, Metal-bindingUniRule annotationSAAS annotations, Nucleotide-binding

Enzyme and pathway databases

BioCyciPPUT76869:GIXB-4782-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Acetyl-coenzyme A synthetaseUniRule annotation (EC:6.2.1.1UniRule annotation)
Short name:
AcCoA synthetaseUniRule annotation
Short name:
AcsUniRule annotation
Alternative name(s):
Acetate--CoA ligase
Acyl-activating enzyme
Gene namesi
Name:acsAUniRule annotation
Ordered Locus Names:PputGB1_4701Imported
OrganismiPseudomonas putida (strain GB-1)Imported
Taxonomic identifieri76869 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000002157: Chromosome

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei605 – 6051N6-acetyllysine By similarityUniRule annotation

Post-translational modificationi

Acetylated. Deacetylation by the SIR2-homolog deacetylase activates the enzyme By similarity.UniRule annotation

Keywords - PTMi

AcetylationUniRule annotation

Interactioni

Protein-protein interaction databases

STRINGi76869.PputGB1_4701.

Structurei

3D structure databases

ProteinModelPortaliB0KHW7.
SMRiB0KHW7. Positions 21-641.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni407 – 4126Substrate binding By similarityUniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0365.
HOGENOMiHOG000229981.
KOiK01895.
OMAiRRIFEPT.
OrthoDBiEOG68WR2H.

Family and domain databases

HAMAPiMF_01123. Ac_CoA_synth.
InterProiIPR011904. Ac_CoA_lig.
IPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view]
PfamiPF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02188. Ac_CoA_lig_AcsA. 1 hit.
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B0KHW7-1 [UniParc]FASTAAdd to Basket

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MFDIRNYPQA LAVSQSATPT PDEYHRLYRQ SVDDPDTFWA EQAKRLDWIK    50
PWSSVQQCDL NTGKARWFDG AQLNVSYNCI DRHLAQRGEQ TALLWEGDDP 100
KDSKAITYRE LHRQVCRLAN AMKARGVKKG DRVCIYMPMI PEAAFAMLAC 150
TRIGAIHSVV FGGFSPDALR DRILDADCRT VITADEGVRG GKRVPLKQNV 200
DKALASCPAV SSVLVVRRTG GDVAWAEGRD LWYHEVTEKA GDDCPPEPMA 250
AEDPLFILYT SGSTGKPKGV LHTTGGYLLQ ATMTFKVVFD YRDGEVFWCT 300
ADVGWVTGHS YIVYGPLANG AISLMFEGVP NYPDTSRFWQ VVDKHQVNIF 350
YTAPTALRAL MREGSAPLQG TSRKSLRLLG SVGEPINPEA WEWYFEAVGQ 400
KRCPIVDTWW QTETGGIMLT PLPGSQALKP GCATQPMFGV QPALLDEKGK 450
LIEGPGAGLL VIKASWPGQI RSVYGDHQRM VDTYFKPMPG YYFTGDGARR 500
DADGDYWITG RIDDVINVSG HRIGTAEVES ALVLHDSVAE AAVVGYPHDL 550
KGQGVYAFVT TMNGVTPDDT LKAELLALVS KEIGSFAKPE LIQWAPALPK 600
TRSGKIMRRI LRKIACNELD NLGDTSTLAD PSVVQGLIDK RLNQ 644
Length:644
Mass (Da):71,051
Last modified:March 18, 2008 - v1
Checksum:i6DA55322F09A7A93
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000926 Genomic DNA. Translation: ABZ00588.1.
RefSeqiYP_001670923.1. NC_010322.1.

Genome annotation databases

EnsemblBacteriaiABZ00588; ABZ00588; PputGB1_4701.
GeneIDi5872512.
KEGGippg:PputGB1_4701.
PATRICi19936495. VBIPsePut76638_4709.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000926 Genomic DNA. Translation: ABZ00588.1 .
RefSeqi YP_001670923.1. NC_010322.1.

3D structure databases

ProteinModelPortali B0KHW7.
SMRi B0KHW7. Positions 21-641.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 76869.PputGB1_4701.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABZ00588 ; ABZ00588 ; PputGB1_4701 .
GeneIDi 5872512.
KEGGi ppg:PputGB1_4701.
PATRICi 19936495. VBIPsePut76638_4709.

Phylogenomic databases

eggNOGi COG0365.
HOGENOMi HOG000229981.
KOi K01895.
OMAi RRIFEPT.
OrthoDBi EOG68WR2H.

Enzyme and pathway databases

BioCyci PPUT76869:GIXB-4782-MONOMER.

Family and domain databases

HAMAPi MF_01123. Ac_CoA_synth.
InterProi IPR011904. Ac_CoA_lig.
IPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view ]
Pfami PF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR02188. Ac_CoA_lig_AcsA. 1 hit.
PROSITEi PS00455. AMP_BINDING. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: GB-1Imported.

Entry informationi

Entry nameiB0KHW7_PSEPG
AccessioniPrimary (citable) accession number: B0KHW7
Entry historyi
Integrated into UniProtKB/TrEMBL: March 18, 2008
Last sequence update: March 18, 2008
Last modified: June 11, 2014
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome

External Data

Dasty 3

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