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B0KHQ8 (RLMG_PSEPG) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 28. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Ribosomal RNA large subunit methyltransferase G
EC=2.1.1.174
Alternative name(s):
23S rRNA m2G1835 methyltransferase
rRNA (guanine-N(2)-)-methyltransferase RlmG
Gene names
Name:rlmG
Ordered Locus Names:PputGB1_4642
OrganismPseudomonas putida (strain GB-1) [Complete proteome] [HAMAP]
Taxonomic identifier76869 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length374 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Specifically methylates the guanosine in position 1835 (m2G1835) of 23S rRNA By similarity. HAMAP MF_01859

Catalytic activity

S-adenosyl-L-methionine + guanosine(1835) in 23S rRNA = S-adenosyl-L-homocysteine + N(2)-methylguanosine(1835) in 23S rRNA. HAMAP MF_01859

Subcellular location

Cytoplasm Potential HAMAP MF_01859.

Sequence similarities

Belongs to the methyltransferase superfamily. RlmG family.

Ontologies

Keywords
   Biological processrRNA processing
   Cellular componentCytoplasm
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionnucleic acid binding

Inferred from electronic annotation. Source: InterPro

rRNA (guanine-N2-)-methyltransferase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 374374Ribosomal RNA large subunit methyltransferase G HAMAP MF_01859
PRO_0000366480

Sequences

Sequence LengthMass (Da)Tools
B0KHQ8 [UniParc].

Last modified March 18, 2008. Version 1.
Checksum: 24C75D890092DA13

FASTA37440,626
        10         20         30         40         50         60 
MPLLTTPYAE LDLIRQPEQA NDPLQAFDAA DEYLLAQLHE QAPDANCRVL VLNDSFGALA 

        70         80         90        100        110        120 
VSLAGRLQVV SSGDSHLAHL GLEKNLTRNG LTFDSVPFVP ASEHWQGLFD RVLVRVPKTL 

       130        140        150        160        170        180 
ALLEEQLIRL QGHLAPGAQV IAGAMIKHLP RAAGDLMEKY IGPVQASLAQ KKARLLTATV 

       190        200        210        220        230        240 
AERPLARSPY PSRYHLDAPA LELVNHANVF CREGLDIGTR AFLPHLPRDL GNARVADLGC 

       250        260        270        280        290        300 
GNGVLAIASA LGNPDAEYTL VDESYMAVQS AQENWRAALG ERAANFVAAD GLAGVEKQSL 

       310        320        330        340        350        360 
DVVLCNPPFH QQQVVGDFLA WRMFQQAREA LVVGGALYIV GNRHLGYHSK LARLFRGVEQ 

       370 
VAATPKFVIL KARK

« Hide

References

[1]"Complete sequence of Pseudomonas putida GB-1."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Bruce D., Goodwin L., Chertkov O., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M. expand/collapse author list , Hauser L., Kyrpides N., Kim E., McCarthy J.K., Richardson P.
Submitted (JAN-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: GB-1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000926 Genomic DNA. Translation: ABZ00529.1.
RefSeqYP_001670864.1. NC_010322.1.

3D structure databases

ProteinModelPortalB0KHQ8.
ModBaseSearch...

Protein-protein interaction databases

STRINGB0KHQ8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID5872450.
GenomeReviewsGene locus PputGB1_4642 in contig CP000926_GR.
KEGGppg:PputGB1_4642.
PATRIC19936367. VBIPsePut76638_4648.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG321931.
OMANRHLGYH.
ProtClustDBCLSK868782.

Enzyme and pathway databases

BioCycPPUT76869:PPUTGB1_4642-MONOMER.

Family and domain databases

HAMAPMF_01859. 23SrRNA_methyltr_G.
[Tree]
InterProIPR002052. DNA_methylase_N6_adenine_CS.
IPR017237. rRNA_m2G-MeTrfase_RsmD.
IPR007848. Small_mtfrase.
[Graphical view]
KOK00564.
PfamPF05175. MTS. 1 hit.
[Graphical view]
PIRSFPIRSF037565. RRNA_m2G_Mtase_RsmD_prd. 1 hit.
ProtoNetSearch...

Entry information

Entry nameRLMG_PSEPG
AccessionPrimary (citable) accession number: B0KHQ8
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: March 18, 2008
Last modified: January 25, 2012
This is version 28 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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