Reviewed, UniProtKB/Swiss-Prot B0KH90 (STHA_PSEPG)

Names and origin

Protein names

Recommended name:
    Soluble pyridine nucleotide transhydrogenase
      Short name=STH
    EC=1.6.1.1
Alternative name(s):
    NAD(P)(+) transhydrogenase [B-specific]

Gene names

Name:	sthA
Ordered Locus Names:	PputGB1_1692

Organism

Pseudomonas putida (strain GB-1) [Complete proteome] [HAMAP]

Taxonomic identifier

76869 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	464 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Conversion of NADPH, generated by peripheral catabolic pathways, to NADH, which can enter the respiratory chain for energy generation By similarity.
Catalytic activity	NADPH + NAD(+) = NADP(+) + NADH.
Cofactor	Binds 1 FAD per subunit By similarity.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein NAD NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NADP metabolic process Inferred from electronic annotation. Source: HAMAP cell redox homeostasis Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro NAD(P)+ transhydrogenase (B-specific) activity Inferred from electronic annotation. Source: HAMAP electron carrier activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 464	464	Soluble pyridine nucleotide transhydrogenase		PRO_1000078412
Regions
	Nucleotide binding	35 – 44	10	FAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	B0KH90-1 [UniParc]. Last modified March 18, 2008. Version 1. Checksum: 32DA105F9B0C39AD	FASTA	464	50,949
	10 20 30 40 50 60 MAVYNYDVVV LGSGPAGEGA AMNAAKAGRK VAMVDDRRQV GGNCTHLGTI PSKALRHSVR 70 80 90 100 110 120 QIMQFNTNPM FRAIGEPRWF SFPDVLKSAE KVISKQVASR TGYYARNRVD VFFGTGSFAD 130 140 150 160 170 180 EQTVEVVCPN GVVEKLNAKH IIIATGSRPY RPADIDFHHP RVYDSDTILS LSHTPRKLIV 190 200 210 220 230 240 YGAGVIGCEY ASIFSGLGVL VELVDNRGQL LSFLDSEISQ ALSYHFSNNN ITVRHNEEYE 250 260 270 280 290 300 RVEGLDNGVI LHLKSGKKIK ADALLWCNGR TGNTDKLGLE NIGIKVNSRG QIEVDEAYRT 310 320 330 340 350 360 TVPNIYGAGD VIGWPSLASA AHDQGRSAAG SIVDNGSWRF VNDVPTGIYT IPEISSIGKN 370 380 390 400 410 420 EQELTQAKVP YEVGKAFFKG MARAQIAGEP QGMLKILFHR ETLEILGVHC FGYQASEIVH 430 440 450 460 IGQAIMNQPG EHNNLKYFVN TTFNYPTMAE AYRVAAYDGL NRLF

References

[1]

"Complete sequence of Pseudomonas putida GB-1." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Bruce D., Goodwin L., Chertkov O., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., McCarthy J.K., Richardson P. Submitted (JAN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000926 Genomic DNA. Translation: ABY97595.1.
RefSeq	YP_001667931.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5869472.
GenomeReviews	Gene locus PputGB1_1692 in contig CP000926_GR.
KEGG	ppg:PputGB1_1692.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_00247. [Tree]
InterPro	IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR000815. Hg_reductase. IPR001100. Pyr_nuc-diS_OxRdtase. IPR004099. Pyr_nucl-diS_OxRdtase_dimer. IPR001327. Pyr_OxRdtase_NAD_bd. [Graphical view]
Gene3D	G3DSA:3.30.390.30. Pyr_redox_dim. 1 hit.
Pfam	PF00070. Pyr_redox. 1 hit. PF07992. Pyr_redox_2. 1 hit. PF02852. Pyr_redox_dim. 1 hit. [Graphical view]
PRINTS	PR00368. FADPNR. PR00945. HGRDTASE. PR00411. PNDRDTASEI.
ProDom	PD000139. FAD_pyr_redox. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

STHA_PSEPG

Accession

Primary (citable) accession number: B0KH90

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	March 18, 2008
Last modified:	November 25, 2008
This is version 8 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)