B0KGU6 (HGD_PSEPG) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Homogentisate 1,2-dioxygenase
EC=1.13.11.5

Alternative name(s):
Homogentisate oxygenase
Homogentisic acid oxidase
Homogentisicase

Gene names

Name:	hmgA
Ordered Locus Names:	PputGB1_4605

Organism

Pseudomonas putida (strain GB-1) [Complete proteome] [HAMAP]

Taxonomic identifier

76869 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas putida group › Pseudomonas putida

Protein attributes

Sequence length	433 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Homogentisate + O2 = 4-maleylacetoacetate. HAMAP-Rule MF_00334
Cofactor	Iron By similarity.
Pathway	Amino-acid degradation; L-phenylalanine degradation; acetoacetate and fumarate from L-phenylalanine: step 4/6. HAMAP-Rule MF_00334
Sequence similarities	Belongs to the homogentisate dioxygenase family.

Ontologies

Keywords
Biological process	Phenylalanine catabolism Tyrosine catabolism
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	L-phenylalanine catabolic process Inferred from electronic annotation. Source: HAMAP tyrosine catabolic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	homogentisate 1,2-dioxygenase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 433	433	Homogentisate 1,2-dioxygenase HAMAP-Rule MF_00334		PRO_1000079266
Sites
	Metal binding	331	1	Iron By similarity
	Metal binding	337	1	Iron By similarity
	Metal binding	367	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	B0KGU6 [UniParc]. Last modified March 18, 2008. Version 1. Checksum: 2F7E6F29323464BC	FASTA	433	48,079
	10 20 30 40 50 60 MNRDTSPDLH YLSGFGNEFA SEALPGALPV GQNSPQKAPY GLYAELLSGT AFTMARSELR 70 80 90 100 110 120 RTWLYRIRPS ALHPRFERLA RQPLSGPLGG INPNRLRWSP QPIPAEPTDF IEGWLPMVAN 130 140 150 160 170 180 AAAEKPAGVS IYIYRANRSM ERVFFNADGE LLLVPEQGRL RIATELGVME VEPLEIAVIP 190 200 210 220 230 240 RGMKFRVELL DGQARGYIAE NHGAPLRIPD LGPIGSNGLA NPRDFLTPVA HYEEAEAPVQ 250 260 270 280 290 300 LVQKFLGEHW ACELQHSPLD VVAWHGSNVP YKYDLRRFNT IGTVSFDHPD PSIFTVLTSP 310 320 330 340 350 360 TSVHGLANMD FVIFPPRWMV AENTFRPPWF HRNLMNEFMG LINGAYDAKA EGFLPGGASL 370 380 390 400 410 420 HGVMSAHGPD AETCEKAIAA DLAPHKIDNT MAFMFETSQV LRPSLQALEC PQLQADYDSC 430 WATLPSTFNP NRR

References

[1]

"Complete sequence of Pseudomonas putida GB-1." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Bruce D., Goodwin L., Chertkov O., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., McCarthy J.K., Richardson P. Submitted (JAN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: GB-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000926 Genomic DNA. Translation: ABZ00492.1.
RefSeq	YP_001670827.1. NC_010322.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	76869.PputGB1_4605.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABZ00492; ABZ00492; PputGB1_4605.
GeneID	5872413.
KEGG	ppg:PputGB1_4605.
PATRIC	19936289. VBIPsePut76638_4609.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG3508.
HOGENOM	HOG000139824.
KO	K00451.
OMA	AVTKWGL.
ProtClustDB	PRK05341.
Enzyme and pathway databases
BioCyc	PPUT76869:GIXB-4683-MONOMER.
UniPathway	UPA00139; UER00339.
Family and domain databases
Gene3D	2.60.120.10. 2 hits.
HAMAP	MF_00334. Homogentis_dioxygen.
InterPro	IPR005708. Homogentis_dOase. IPR022950. Homogentis_dOase_bac. IPR014710. RmlC-like_jellyroll. IPR011051. RmlC_Cupin. [Graphical view]
PANTHER	PTHR11056. PTHR11056. 1 hit.
Pfam	PF04209. HgmA. 1 hit. [Graphical view]
SUPFAM	SSF51182. RmlC_like_cupin. 1 hit.
TIGRFAMs	TIGR01015. hmgA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HGD_PSEPG

Accession

Primary (citable) accession number: B0KGU6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	March 18, 2008
Last modified:	May 1, 2013
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program