B0KF82 (LEUD_PSEPG) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-isopropylmalate dehydratase small subunit
EC=4.2.1.33

Alternative name(s):
Alpha-IPM isomerase
Short name=IPMI
Isopropylmalate isomerase

Gene names

Name:	leuD
Ordered Locus Names:	PputGB1_1517

Organism

Pseudomonas putida (strain GB-1) [Complete proteome] [HAMAP]

Taxonomic identifier

76869 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas putida group › Pseudomonas putida

Protein attributes

Sequence length	214 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate By similarity. HAMAP-Rule MF_01031
Catalytic activity	(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate. HAMAP-Rule MF_01031
Pathway	Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 2/4. HAMAP-Rule MF_01031
Subunit structure	Heterodimer of LeuC and LeuD By similarity.
Sequence similarities	Belongs to the LeuD family. LeuD type 1 subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis Leucine biosynthesis
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	leucine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular_component	3-isopropylmalate dehydratase complex Inferred from electronic annotation. Source: InterPro
Molecular_function	3-isopropylmalate dehydratase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 214	214	3-isopropylmalate dehydratase small subunit HAMAP-Rule MF_01031		PRO_1000084260

Sequences

	Sequence		Length	Mass (Da)
	B0KF82 [UniParc]. Last modified March 18, 2008. Version 1. Checksum: 200A415F6BEDB1DB	FASTA	214	24,225
	10 20 30 40 50 60 MKAFTQHTGL VAPLDRANVD TDQIIPKQFL KSIKRTGFGP NLFDEWRYLD VGQPYQDNSK 70 80 90 100 110 120 RPVNQEFVLN HARYQGASVL LARENFGCGS SREHAPWALD EYGFRSIIAP SFADIFFNNS 130 140 150 160 170 180 FKNGLLPIIL SDEEVDELFK QVEANPGYQL TIDLQAQAVT RPDGKVLHFE IDAFRKHCLL 190 200 210 NGLDDIGLTL QDSDAIKAFE GKHRAGQPWL FRDA

References

[1]

"Complete sequence of Pseudomonas putida GB-1." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Bruce D., Goodwin L., Chertkov O., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., McCarthy J.K., Richardson P. Submitted (JAN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: GB-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000926 Genomic DNA. Translation: ABY97422.1.
RefSeq	YP_001667758.1. NC_010322.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	76869.PputGB1_1517.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABY97422; ABY97422; PputGB1_1517.
GeneID	5869293.
KEGG	ppg:PputGB1_1517.
PATRIC	19930053. VBIPsePut76638_1529.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0066.
HOGENOM	HOG000222939.
KO	K01704.
OMA	YQDNSKR.
ProtClustDB	PRK01641.
Enzyme and pathway databases
BioCyc	PPUT76869:GIXB-1564-MONOMER.
UniPathway	UPA00048; UER00071.
Family and domain databases
Gene3D	3.20.19.10. 1 hit.
HAMAP	MF_01031. LeuD_type1.
InterPro	IPR004431. 3-IsopropMal_deHydase_ssu. IPR015937. Acoase/IPM_deHydtase. IPR015928. Aconitase/3IPM_dehydase_swvl. IPR000573. AconitaseA/IPMdHydase_ssu_swvl. [Graphical view]
PANTHER	PTHR11670. PTHR11670. 1 hit. PTHR11670:SF2. PTHR11670:SF2. 1 hit.
Pfam	PF00694. Aconitase_C. 1 hit. [Graphical view]
SUPFAM	SSF52016. Aconitase/3IPM_dehydase_swvl. 1 hit.
TIGRFAMs	TIGR00171. leuD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LEUD_PSEPG

Accession

Primary (citable) accession number: B0KF82

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	March 18, 2008
Last modified:	May 1, 2013
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program