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Protein

Formate--tetrahydrofolate ligase

Gene

fhs

Organism
Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) (Clostridium thermohydrosulfuricum)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate.UniRule annotation

Pathway:itetrahydrofolate interconversion

This protein is involved in the pathway tetrahydrofolate interconversion, which is part of One-carbon metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway tetrahydrofolate interconversion and in One-carbon metabolism.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi65 – 728ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

One-carbon metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciTPSE340099:GH4W-310-MONOMER.
UniPathwayiUPA00193.

Names & Taxonomyi

Protein namesi
Recommended name:
Formate--tetrahydrofolate ligaseUniRule annotation (EC:6.3.4.3UniRule annotation)
Alternative name(s):
Formyltetrahydrofolate synthetaseUniRule annotation
Short name:
FHSUniRule annotation
Short name:
FTHFSUniRule annotation
Gene namesi
Name:fhsUniRule annotation
Ordered Locus Names:Teth39_0303
OrganismiThermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) (Clostridium thermohydrosulfuricum)
Taxonomic identifieri340099 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaThermoanaerobacteralesThermoanaerobacteraceaeThermoanaerobacter
ProteomesiUP000002156 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 555555Formate--tetrahydrofolate ligasePRO_1000146706Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi340099.Teth39_0303.

Structurei

3D structure databases

ProteinModelPortaliB0KC36.
SMRiB0KC36. Positions 4-553.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the formate--tetrahydrofolate ligase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG2759.
HOGENOMiHOG000040280.
KOiK01938.
OMAiCGEIMTM.
OrthoDBiEOG6PCPSP.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
HAMAPiMF_01543. FTHFS.
InterProiIPR000559. Formate_THF_ligase.
IPR020628. Formate_THF_ligase_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01268. FTHFS. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00721. FTHFS_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B0KC36-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKSDIEIAQE AKMLHIREVA KKLDIEEDYL EYYGKYKAKI SPALSEKIKD
60 70 80 90 100
RKDGKLILVT AITPTPAGEG KTTLTVGLGQ ALAKIGKKAM IALREPSLGP
110 120 130 140 150
CMGIKGGAAG GGYSQVVPME DINLHFTGDL HAITAAHNLL AAMIDNHIHH
160 170 180 190 200
GNELNIDIRA ITWKRAMDMN DRALREIIVG LGGKANGFPR QDGFIITVAS
210 220 230 240 250
EVMAILCLAQ DLMDLKRRIG DIIVAYDKDG NPVTARDLKA DGAMTVLLKD
260 270 280 290 300
AIKPNLVQTI ENVPAFVHGG PFANIAHGCN SLIATKYGLK LADYLVTEAG
310 320 330 340 350
FGADLGAEKF FDIKSRFGGL TPNAAVIVAT VKALKMHGGV KKEHLQKEDV
360 370 380 390 400
EAVRRGIENL EKQVENVRKF GVPVVVALNR FVFDTEREIE EVRKACEEMG
410 420 430 440 450
VDMAVAEVWE KGGEGGIELA EKVVKACETP SNFHVLYDET LPIKDKLHII
460 470 480 490 500
ATEIYGADGV EYTASALKDI ANLERLGFDK MPIVVAKTQY SLSDDPKLLG
510 520 530 540 550
RPRGFKITVR ELRVSRGAGF IVALTGDIMT MPGLPKHPAA ENIDIDENGR

ITGLF
Length:555
Mass (Da):60,349
Last modified:March 18, 2008 - v1
Checksum:iFE053937AE11690F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000924 Genomic DNA. Translation: ABY93972.1.
RefSeqiWP_012268968.1. NC_010321.1.

Genome annotation databases

EnsemblBacteriaiABY93972; ABY93972; Teth39_0303.
KEGGitpd:Teth39_0303.
PATRICi23885169. VBIThePse6203_0323.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000924 Genomic DNA. Translation: ABY93972.1.
RefSeqiWP_012268968.1. NC_010321.1.

3D structure databases

ProteinModelPortaliB0KC36.
SMRiB0KC36. Positions 4-553.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi340099.Teth39_0303.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABY93972; ABY93972; Teth39_0303.
KEGGitpd:Teth39_0303.
PATRICi23885169. VBIThePse6203_0323.

Phylogenomic databases

eggNOGiCOG2759.
HOGENOMiHOG000040280.
KOiK01938.
OMAiCGEIMTM.
OrthoDBiEOG6PCPSP.

Enzyme and pathway databases

UniPathwayiUPA00193.
BioCyciTPSE340099:GH4W-310-MONOMER.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
HAMAPiMF_01543. FTHFS.
InterProiIPR000559. Formate_THF_ligase.
IPR020628. Formate_THF_ligase_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01268. FTHFS. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00721. FTHFS_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete sequence of Thermoanaerobacter pseudethanolicus 39E."
    Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Bruce D., Goodwin L., Saunders E., Brettin T., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L.
    , Kyrpides N., Lykidis A., Hemme C., Fields M.W., He Z., Zhou J., Richardson P.
    Submitted (JAN-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 33223 / 39E.

Entry informationi

Entry nameiFTHS_THEP3
AccessioniPrimary (citable) accession number: B0KC36
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: March 18, 2008
Last modified: July 22, 2015
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.