B0KC36 (FTHS_THEP3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Formate--tetrahydrofolate ligase
EC=6.3.4.3

Alternative name(s):
Formyltetrahydrofolate synthetase
Short name=FHS
Short name=FTHFS

Gene names

Name:	fhs
Ordered Locus Names:	Teth39_0303

Organism

Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) (Clostridium thermohydrosulfuricum) [Complete proteome] [HAMAP]

Taxonomic identifier

340099 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Clostridia › Thermoanaerobacterales › Thermoanaerobacteraceae › Thermoanaerobacter › Thermoanaerobacter pseudethanolicus

Protein attributes

Sequence length	555 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate. HAMAP-Rule MF_01543
Pathway	One-carbon metabolism; tetrahydrofolate interconversion. HAMAP-Rule MF_01543
Sequence similarities	Belongs to the formate--tetrahydrofolate ligase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	folic acid-containing compound biosynthetic process Inferred from electronic annotation. Source: InterPro tetrahydrofolate interconversion Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP formate-tetrahydrofolate ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 555	555	Formate--tetrahydrofolate ligase HAMAP-Rule MF_01543		PRO_1000146706
Regions
	Nucleotide binding	65 – 72	8	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	B0KC36 [UniParc]. Last modified March 18, 2008. Version 1. Checksum: FE053937AE11690F	FASTA	555	60,349
	10 20 30 40 50 60 MKSDIEIAQE AKMLHIREVA KKLDIEEDYL EYYGKYKAKI SPALSEKIKD RKDGKLILVT 70 80 90 100 110 120 AITPTPAGEG KTTLTVGLGQ ALAKIGKKAM IALREPSLGP CMGIKGGAAG GGYSQVVPME 130 140 150 160 170 180 DINLHFTGDL HAITAAHNLL AAMIDNHIHH GNELNIDIRA ITWKRAMDMN DRALREIIVG 190 200 210 220 230 240 LGGKANGFPR QDGFIITVAS EVMAILCLAQ DLMDLKRRIG DIIVAYDKDG NPVTARDLKA 250 260 270 280 290 300 DGAMTVLLKD AIKPNLVQTI ENVPAFVHGG PFANIAHGCN SLIATKYGLK LADYLVTEAG 310 320 330 340 350 360 FGADLGAEKF FDIKSRFGGL TPNAAVIVAT VKALKMHGGV KKEHLQKEDV EAVRRGIENL 370 380 390 400 410 420 EKQVENVRKF GVPVVVALNR FVFDTEREIE EVRKACEEMG VDMAVAEVWE KGGEGGIELA 430 440 450 460 470 480 EKVVKACETP SNFHVLYDET LPIKDKLHII ATEIYGADGV EYTASALKDI ANLERLGFDK 490 500 510 520 530 540 MPIVVAKTQY SLSDDPKLLG RPRGFKITVR ELRVSRGAGF IVALTGDIMT MPGLPKHPAA 550 ENIDIDENGR ITGLF

References

[1]

"Complete sequence of Thermoanaerobacter pseudethanolicus 39E." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Bruce D., Goodwin L., Saunders E., Brettin T., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Lykidis A., Hemme C., Fields M.W., He Z., Zhou J., Richardson P. Submitted (JAN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33223 / 39E.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000924 Genomic DNA. Translation: ABY93972.1.
RefSeq	YP_001664308.1. NC_010321.1.
3D structure databases
ProteinModelPortal	B0KC36.
SMR	B0KC36. Positions 4-553.
ModBase	Search...
Protein-protein interaction databases
STRING	340099.Teth39_0303.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABY93972; ABY93972; Teth39_0303.
GeneID	5874250.
KEGG	tpd:Teth39_0303.
PATRIC	23885169. VBIThePse6203_0323.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2759.
HOGENOM	HOG000040280.
KO	K01938.
OMA	EIMAVLC.
ProtClustDB	CLSK901681.
Enzyme and pathway databases
UniPathway	UPA00193.
Family and domain databases
HAMAP	MF_01543. FTHFS.
InterPro	IPR000559. Formate_THF_ligase. IPR020628. Formate_THF_ligase_CS. [Graphical view]
Pfam	PF01268. FTHFS. 1 hit. [Graphical view]
PROSITE	PS00721. FTHFS_1. 1 hit. PS00722. FTHFS_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FTHS_THEP3

Accession

Primary (citable) accession number: B0KC36

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	March 18, 2008
Last modified:	May 1, 2013
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program