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B0KBI1 (DDL_THEP3) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 44. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:Teth39_0187
OrganismThermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) (Clostridium thermohydrosulfuricum) [Complete proteome] [HAMAP]
Taxonomic identifier340099 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaThermoanaerobacteralesThermoanaerobacteraceaeThermoanaerobacter

Protein attributes

Sequence length359 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 359359D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_0000341184

Regions

Domain141 – 346206ATP-grasp
Nucleotide binding172 – 22756ATP By similarity

Sites

Metal binding2991Magnesium or manganese 1 By similarity
Metal binding3131Magnesium or manganese 1 By similarity
Metal binding3131Magnesium or manganese 2 By similarity
Metal binding3151Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
B0KBI1 [UniParc].

Last modified March 18, 2008. Version 1.
Checksum: A8646128A86188AE

FASTA35940,083
        10         20         30         40         50         60 
MEKKKIAVLF GGQSGEHEVS LMSAKSIINN LDKDKYEIYM VGITKKGEWY LYRGDVGKIE 

        70         80         90        100        110        120 
TGEWEKEGIP AIMGASTKYR GIITFEDGEN GFYPIDVVFP VLHGPNGEDG TIQGLLELLD 

       130        140        150        160        170        180 
MPYVGANVLS SALCMDKVFT KRIFKEAGLP TPDFVVVYGK EIEDLEAIKK KIEHLGYPCF 

       190        200        210        220        230        240 
VKPANLGSSV GITKVHNEEE LPGALKLAAK YDRKLLIERG IDAREIECSV LGNENPEASI 

       250        260        270        280        290        300 
AGEIVPSNEF YDYNAKYFDG GKSLLLIPAP LPDEKMEEVR KLAIKAYKAL DLRGMARVDF 

       310        320        330        340        350 
LMDRNTGTLY LNEVNTIPGF TKISMYPKLW ESSGKSYSTL LDELINLAVE SHNEKCREW 

« Hide

References

[1]"Complete sequence of Thermoanaerobacter pseudethanolicus 39E."
Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Bruce D., Goodwin L., Saunders E., Brettin T., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L. expand/collapse author list , Kyrpides N., Lykidis A., Hemme C., Fields M.W., He Z., Zhou J., Richardson P.
Submitted (JAN-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 33223 / 39E.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000924 Genomic DNA. Translation: ABY93860.1.
RefSeqYP_001664196.1. NC_010321.1.

3D structure databases

ProteinModelPortalB0KBI1.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING340099.Teth39_0187.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABY93860; ABY93860; Teth39_0187.
GeneID5875521.
KEGGtpd:Teth39_0187.
PATRIC23884914. VBIThePse6203_0200.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011593.
KOK01921.
OMAMDKIAMK.
OrthoDBEOG64BQ73.
ProtClustDBPRK01966.

Enzyme and pathway databases

BioCycTPSE340099:GH4W-190-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_THEP3
AccessionPrimary (citable) accession number: B0KBI1
Entry history
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: March 18, 2008
Last modified: February 19, 2014
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways