Reviewed, UniProtKB/Swiss-Prot B0KAI2 (RIBBA_THEP3)

Names and origin

Protein names

Recommended name:
    Riboflavin biosynthesis protein ribBA
Including the following 2 domains:
    1- Recommended name:
            3,4-dihydroxy-2-butanone 4-phosphate synthase
                Short name=DHBP synthase
              EC=4.1.99.12
    2- Recommended name:
            GTP cyclohydrolase-2
              EC=3.5.4.25
        Alternative name(s):
            GTP cyclohydrolase II

Gene names

Name:	ribBA
Ordered Locus Names:	Teth39_0023

Organism

Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) (Clostridium thermohydrosulfuricum) [Complete proteome] [HAMAP]

Taxonomic identifier

340099 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Thermoanaerobacterales › Thermoanaerobacteriaceae › Thermoanaerobacter

Protein attributes

Sequence length	396 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate By similarity. HAMAP MF_01283 Catalyzes the conversion of GTP to 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate By similarity. HAMAP MF_01283
Catalytic activity	D-ribulose 5-phosphate = formate + L-3,4-dihydroxybutan-2-one 4-phosphate. HAMAP MF_01283 GTP + 3 H2O = formate + 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)pyrimidine + diphosphate. HAMAP MF_01283
Cofactor	Binds 2 divalent metal cations per subunit. Magnesium or manganese By similarity. HAMAP MF_01283 Binds 1 zinc ion per subunit By similarity. HAMAP MF_01283
Pathway	Cofactor biosynthesis; riboflavin biosynthesis; 2-hydroxy-3-oxobutyl phosphate from D-ribulose 5-phosphate: step 1/1. HAMAP MF_01283 Cofactor biosynthesis; riboflavin biosynthesis; 5-amino-6-(D-ribitylamino)uracil from GTP: step 1/4. HAMAP MF_01283
Sequence similarities	In the N-terminal section; belongs to the DHBP synthase family. In the C-terminal section; belongs to the GTP cyclohydrolase II family.

Ontologies

Keywords
Biological process	Riboflavin biosynthesis
Ligand	GTP-binding Magnesium Manganese Metal-binding Nucleotide-binding Zinc
Molecular function	Hydrolase Lyase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	riboflavin biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	3,4-dihydroxy-2-butanone-4-phosphate synthase activity Inferred from electronic annotation. Source: HAMAP GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTP cyclohydrolase II activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP manganese ion binding Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 396	396	Riboflavin biosynthesis protein ribBA HAMAP MF_01283		PRO_1000140367
Regions
	Nucleotide binding	249 – 253	5	GTP By similarity
	Nucleotide binding	291 – 293	3	GTP By similarity
	Region	1 – 199	199	DHBP synthase HAMAP MF_01283
	Region	26 – 27	2	D-ribulose 5-phosphate binding By similarity
	Region	138 – 142	5	D-ribulose 5-phosphate binding By similarity
	Region	200 – 396	197	GTP cyclohydrolase II HAMAP MF_01283
Sites
	Active site	325	1	Proton acceptor; for GTP cyclohydrolase activity Potential
	Active site	327	1	Nucleophile; for GTP cyclohydrolase activity By similarity
	Metal binding	27	1	Magnesium or manganese 1 By similarity
	Metal binding	27	1	Magnesium or manganese 2 By similarity
	Metal binding	141	1	Magnesium or manganese 2 By similarity
	Metal binding	254	1	Zinc; catalytic By similarity
	Metal binding	265	1	Zinc; catalytic By similarity
	Metal binding	267	1	Zinc; catalytic By similarity
	Binding site	31	1	D-ribulose 5-phosphate By similarity
	Binding site	162	1	D-ribulose 5-phosphate By similarity
	Binding site	270	1	GTP By similarity
	Binding site	313	1	GTP By similarity
	Binding site	348	1	GTP By similarity
	Binding site	353	1	GTP By similarity
	Site	124	1	Essential for DHBP synthase activity By similarity
	Site	162	1	Essential for DHBP synthase activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	B0KAI2-1 [UniParc]. Last modified March 18, 2008. Version 1. Checksum: 089B2F43178A8D60	FASTA	396	44,558
	10 20 30 40 50 60 MFDRIEDAIE DIKQGKMIIV VDDENRENEG DLVMAAEKVT GEHINFMIKY GRGLVCVPMT 70 80 90 100 110 120 EKRLNELGIY QMVENNTDHK ETAFTVSVDY KECTTGISAF ERALTVKKLV DDNSKPEDFT 130 140 150 160 170 180 KPGHIFPLRA KDGGVLVRAG HTEAAVDLAV LAGLKPAGVI CEIIKDDGNM ARLPDLLEFA 190 200 210 220 230 240 KKFGLRIISI EDLIKYRMKN EILVRRVAQA KLPTKYGNFE IVGYEEILTR KQHVALIKGD 250 260 270 280 290 300 INKEPVLVRI HSECLTGDIL GSLRCDCGDQ LHAAMERIGQ EGGVLVYLRQ EGRGIGLLNK 310 320 330 340 350 360 IKAYHLQDQG LDTVEANIKL GFPPDLREYS IAAQILKDIG VKKIRIMTNN PQKIMELSDY 370 380 390 GLDVVERVSI EICPNHYNEK YLKTKKERMG HLILEV

References

[1]

"Complete sequence of Thermoanaerobacter pseudethanolicus 39E." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Bruce D., Goodwin L., Saunders E., Brettin T., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Lykidis A., Hemme C., Fields M.W., He Z., Zhou J., Richardson P. Submitted (JAN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000924 Genomic DNA. Translation: ABY93696.1.
RefSeq	YP_001664032.1.
3D structure databases
SMR	B0KAI2. Positions 1-199, 203-372.
ModBase	Search...
Genome annotation databases
GeneID	5875623.
GenomeReviews	Gene locus Teth39_0023 in contig CP000924_GR.
KEGG	tpd:Teth39_0023.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG735778.
OMA	LRCDCRM.
Family and domain databases
HAMAP	MF_01283. RibBA. [Tree]
InterPro	IPR017945. DHBP_synth_RibB-like_a/b_dom. IPR000422. DHBP_synthase_RibB. IPR000926. GTP_CycHdrlase_II. IPR016299. Riboflavin_synth_RibA. [Graphical view]
Gene3D	G3DSA:3.90.870.10. DHBP_synth_RibB-like_a/b_dom. 1 hit.
Pfam	PF00926. DHBP_synthase. 1 hit. PF00925. GTP_cyclohydro2. 1 hit. [Graphical view]
PIRSF	PIRSF001259. RibA. 1 hit.
TIGRFAMs	TIGR00505. ribA. 1 hit. TIGR00506. ribB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RIBBA_THEP3

Accession

Primary (citable) accession number: B0KAI2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	March 18, 2008
Last modified:	February 9, 2010
This is version 17 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)