B0K9V5 (B0K9V5_THEP3) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
DNA topoisomerase RuleBase RU000518
EC=5.99.1.2 RuleBase RU000518

Gene names

Ordered Locus Names:

Teth39_1264

Organism

Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) (Clostridium thermohydrosulfuricum) [Complete proteome] [HAMAP]

Taxonomic identifier

340099 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Thermoanaerobacterales › Thermoanaerobacteriaceae › Thermoanaerobacter

Protein attributes

Sequence length	693 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. SAAS SAAS000380
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. RuleBase RU000518 SAAS SAAS005733
Sequence similarities	Belongs to the prokaryotic type I/III topoisomerase family. RuleBase RU004206 Contains 1 Toprim domain. SAAS SAAS023406

Ontologies

Keywords
Ligand	ATP-binding SAAS SAAS005733 DNA-binding RuleBase RU000518 SAAS SAAS005733 Nucleotide-binding
Molecular function	Isomerase Topoisomerase RuleBase RU000518 SAAS SAAS005733
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase type I activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	B0K9V5 [UniParc]. Last modified March 18, 2008. Version 1. Checksum: 517D3DE141ED139F	FASTA	693	78,632
	10 20 30 40 50 60 MAKSLVIVES PAKAKTISKF LGKNFKVAAS MGHVRDLPKS QLGIDIENNF APRYITIRGK 70 80 90 100 110 120 GAIIENLKKE AKDADKIFLA TDPDREGEAI SWHIAQLLNL NIEEPCRIEF HEITKNAVQT 130 140 150 160 170 180 ALKSPRSIDL NLVDAQQARR ILDRLVGYKI SPLLWKKIKR GLSAGRVQSV AARLICDRER 190 200 210 220 230 240 EIEAFVPEEY WSITVILSDK KNSGKFEAKF YGTKEDKVEL KTKEDVDKIL AALSEEYIID 250 260 270 280 290 300 KVKTGVKKRN PSPPFITSTL QQEASRKLGF TAKKTMLIAQ QLYEGVEIKG EGSVGLITYI 310 320 330 340 350 360 RTDSTRVANE AKAEVYKYIV EKFGKGYANP EANYVSKRAN VQDAHEAIRP TSIYRDPESI 370 380 390 400 410 420 KDSLTPDQYK LYKLVWSRFI ASQMAPAIYD TVSMDIVNNG YVFKASGSSI KFLGFMTVYV 430 440 450 460 470 480 EGTDMQEEEE KTVPVLQEGT KLFLEELKPM QHFTQPPPRY TEASLIKELE EKGIGRPSTY 490 500 510 520 530 540 APTISTLLER GYVVKDKKNL KPTELGFIVT DALKEFFSKI VDVKFTAEME EQLDKIEEGQ 550 560 570 580 590 600 VKWYEVVEEF YKDFYKTLKI AEEQMEEIEV KEEVEVTDIK CELCGRNMVV KKGRYGKFLA 610 620 630 640 650 660 CSGFPECKNT KPLYEKVGVK CPKCGGEIVK KKSKKGRTYY ACENAPDCDF ILWDKPVEEK 670 680 690 CPVCGSMLIE KNTKNVHILK CSNPGCDYQK EVK

References

[1]

"Complete sequence of Thermoanaerobacter pseudethanolicus 39E." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Bruce D., Goodwin L., Saunders E., Brettin T., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Lykidis A., Hemme C., Fields M.W., He Z., Zhou J., Richardson P. Submitted (JAN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000924 Genomic DNA. Translation: ABY94918.1.
RefSeq	YP_001665254.1. NC_010321.1.
3D structure databases
ProteinModelPortal	B0K9V5.
ModBase	Search...
Protein-protein interaction databases
STRING	B0K9V5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5875159.
GenomeReviews	Gene locus Teth39_1264 in contig CP000924_GR.
KEGG	tpd:Teth39_1264.
PATRIC	23887206. VBIThePse6203_1314.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG512935.
OMA	IERNIRH.
ProtClustDB	PRK05582.
Enzyme and pathway databases
BioCyc	TPSE340099:TETH39_1264-MONOMER.
Family and domain databases
InterPro	IPR000380. Topo_IA. IPR003601. Topo_IA_2. IPR023406. Topo_IA_AS. IPR013497. Topo_IA_cen. IPR013824. Topo_IA_cen_sub1. IPR013825. Topo_IA_cen_sub2. IPR023405. Topo_IA_core_domain. IPR003602. Topo_IA_DNA-bd. IPR013498. Topo_IA_Znf. IPR005733. TopoI_bac-type. IPR006171. Toprim_domain. [Graphical view]
Gene3D	G3DSA:1.10.460.10. Topo_IA_cen_sub1. 2 hits. G3DSA:2.70.20.10. Topo_IA_cen_sub2. 2 hits.
KO	K03168.
PANTHER	PTHR11390. Topo_IA. 1 hit.
Pfam	PF01131. Topoisom_bac. 1 hit. PF01751. Toprim. 1 hit. PF01396. zf-C4_Topoisom. 2 hits. [Graphical view]
PRINTS	PR00417. PRTPISMRASEI.
SMART	SM00437. TOP1Ac. 1 hit. SM00436. TOP1Bc. 1 hit. SM00493. TOPRIM. 1 hit. [Graphical view]
SUPFAM	SSF56712. Topo_IA_core. 1 hit.
TIGRFAMs	TIGR01051. TopA_bact. 1 hit.
PROSITE	PS00396. TOPOISOMERASE_I_PROK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

B0K9V5_THEP3

Accession

Primary (citable) accession number: B0K9V5

Entry history

Integrated into UniProtKB/TrEMBL:	March 18, 2008
Last sequence update:	March 18, 2008
Last modified:	December 14, 2011
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)