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B0K8R7

- SYI_THEP3

UniProt

B0K8R7 - SYI_THEP3

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) (Clostridium thermohydrosulfuricum)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 54 (01 Oct 2014)
      Sequence version 1 (18 Mar 2008)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei559 – 5591Aminoacyl-adenylateUniRule annotation
    Binding sitei603 – 6031ATPUniRule annotation
    Metal bindingi899 – 8991ZincUniRule annotation
    Metal bindingi902 – 9021ZincUniRule annotation
    Metal bindingi919 – 9191ZincUniRule annotation
    Metal bindingi922 – 9221ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciTPSE340099:GH4W-906-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:Teth39_0874
    OrganismiThermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) (Clostridium thermohydrosulfuricum)
    Taxonomic identifieri340099 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesClostridiaThermoanaerobacteralesThermoanaerobacteraceaeThermoanaerobacter
    ProteomesiUP000002156: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 932932Isoleucine--tRNA ligasePRO_1000189210Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi340099.Teth39_0874.

    Structurei

    3D structure databases

    ProteinModelPortaliB0K8R7.
    SMRiB0K8R7. Positions 1-928.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi57 – 6711"HIGH" regionAdd
    BLAST
    Motifi600 – 6045"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiKPVHWCL.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B0K8R7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDYNKTLNLP RTDFPMKANL PTREPEILKR WEEMDIYHKT LEKNKGKEKY    50
    ILHDGPPYAN GDIHIGTAMN KVLKDIIVKY KTMRGYDAPY VPGWDTHGLP 100
    IEQQAIKTLG IKRHEVSPTE FRKVCRDFAF SQIEKQKAQF KRLGVRGDWD 150
    NPYLTLNPEY EAKQIEVFGE MAKKGYIYKG LKPVYWCPSC ETALAEAEIE 200
    YFDETSDSIY VKFRVKDDLG KFKGIVENLN NVYFVIWTTT TWTIPANLAI 250
    ALNPEFDYAL AKFGDEVYIM AKDMLDTVKK EANLSDYEIV AVFKGKDLEG 300
    MKATHPLYDR DSLIILGEHV TLEAGTGCVH TAPGHGEEDF LVGQEYGLEV 350
    LNPIDDKGYF TDKAPGYAGL YYEEANKVIK EDLKKANALV AETRITHSYP 400
    HCWRCKSPII FRATEQWFAS VEGFREEALK AIKEVNWYPS WGEERITNMV 450
    RDRRDWCISR QRVWGVPIPI FYCEKCGKPL INDDTINAVK KIFRQKGSDA 500
    WFEMSAEEIL PKGITCECGS TKFRKETDIM DVWFDSGSSH AAVLQTHPDL 550
    KWPAELYLEG SDQHRGWFQS SLLTSVATRG KAPYRNVLTH GFVVDGEGRK 600
    MSKSLGNGID PADVIKEYGA DILRLWTVSA DFTSDMRISQ EILKQMTEAY 650
    RKIRNTSKFL LSNLYDFDPD KDMLPYEELL EIDKWALFRL NRVVEELTEA 700
    FDKYEYYDFL HLVHTFCVVD MSSLYLDILK DRLYTYPATS KERRAAQTTL 750
    YIILDTLVRL IAPVLTFTSE EIWSYMKHDS QNNFESVQLA DWPQVQEKYN 800
    NPYIIEKWEK LFDIRKDISK ALEIARTDKK IGHSLEAQVD IYPSQELYDF 850
    FKGFNDLEYV FIVSKVVLHQ PEEPAPQNAY ESDDYNLKIV VTHAPGEKCE 900
    RCWMYSETVG TIKEHPTICA RCASHIEQQT QV 932
    Length:932
    Mass (Da):107,851
    Last modified:March 18, 2008 - v1
    Checksum:i0BD53F63FE09E523
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000924 Genomic DNA. Translation: ABY94530.1.
    RefSeqiYP_001664866.1. NC_010321.1.

    Genome annotation databases

    EnsemblBacteriaiABY94530; ABY94530; Teth39_0874.
    GeneIDi5873648.
    KEGGitpd:Teth39_0874.
    PATRICi23886404. VBIThePse6203_0915.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000924 Genomic DNA. Translation: ABY94530.1 .
    RefSeqi YP_001664866.1. NC_010321.1.

    3D structure databases

    ProteinModelPortali B0K8R7.
    SMRi B0K8R7. Positions 1-928.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 340099.Teth39_0874.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABY94530 ; ABY94530 ; Teth39_0874 .
    GeneIDi 5873648.
    KEGGi tpd:Teth39_0874.
    PATRICi 23886404. VBIThePse6203_0915.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi KPVHWCL.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci TPSE340099:GH4W-906-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequence of Thermoanaerobacter pseudethanolicus 39E."
      Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Bruce D., Goodwin L., Saunders E., Brettin T., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L.
      , Kyrpides N., Lykidis A., Hemme C., Fields M.W., He Z., Zhou J., Richardson P.
      Submitted (JAN-2008) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 33223 / 39E.

    Entry informationi

    Entry nameiSYI_THEP3
    AccessioniPrimary (citable) accession number: B0K8R7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: March 18, 2008
    Last modified: October 1, 2014
    This is version 54 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3