SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

B0K8R7

- SYI_THEP3

UniProt

B0K8R7 - SYI_THEP3

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Isoleucine--tRNA ligase

Gene
ileS, Teth39_0874
Organism
Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) (Clostridium thermohydrosulfuricum)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei559 – 5591Aminoacyl-adenylate By similarity
Binding sitei603 – 6031ATP By similarity
Metal bindingi899 – 8991Zinc By similarity
Metal bindingi902 – 9021Zinc By similarity
Metal bindingi919 – 9191Zinc By similarity
Metal bindingi922 – 9221Zinc By similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciTPSE340099:GH4W-906-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:Teth39_0874
OrganismiThermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) (Clostridium thermohydrosulfuricum)
Taxonomic identifieri340099 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaThermoanaerobacteralesThermoanaerobacteraceaeThermoanaerobacter
ProteomesiUP000002156: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 932932Isoleucine--tRNA ligaseUniRule annotationPRO_1000189210Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi340099.Teth39_0874.

Structurei

3D structure databases

ProteinModelPortaliB0K8R7.
SMRiB0K8R7. Positions 1-928.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionUniRule annotationAdd
BLAST
Motifi600 – 6045"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B0K8R7-1 [UniParc]FASTAAdd to Basket

« Hide

MDYNKTLNLP RTDFPMKANL PTREPEILKR WEEMDIYHKT LEKNKGKEKY    50
ILHDGPPYAN GDIHIGTAMN KVLKDIIVKY KTMRGYDAPY VPGWDTHGLP 100
IEQQAIKTLG IKRHEVSPTE FRKVCRDFAF SQIEKQKAQF KRLGVRGDWD 150
NPYLTLNPEY EAKQIEVFGE MAKKGYIYKG LKPVYWCPSC ETALAEAEIE 200
YFDETSDSIY VKFRVKDDLG KFKGIVENLN NVYFVIWTTT TWTIPANLAI 250
ALNPEFDYAL AKFGDEVYIM AKDMLDTVKK EANLSDYEIV AVFKGKDLEG 300
MKATHPLYDR DSLIILGEHV TLEAGTGCVH TAPGHGEEDF LVGQEYGLEV 350
LNPIDDKGYF TDKAPGYAGL YYEEANKVIK EDLKKANALV AETRITHSYP 400
HCWRCKSPII FRATEQWFAS VEGFREEALK AIKEVNWYPS WGEERITNMV 450
RDRRDWCISR QRVWGVPIPI FYCEKCGKPL INDDTINAVK KIFRQKGSDA 500
WFEMSAEEIL PKGITCECGS TKFRKETDIM DVWFDSGSSH AAVLQTHPDL 550
KWPAELYLEG SDQHRGWFQS SLLTSVATRG KAPYRNVLTH GFVVDGEGRK 600
MSKSLGNGID PADVIKEYGA DILRLWTVSA DFTSDMRISQ EILKQMTEAY 650
RKIRNTSKFL LSNLYDFDPD KDMLPYEELL EIDKWALFRL NRVVEELTEA 700
FDKYEYYDFL HLVHTFCVVD MSSLYLDILK DRLYTYPATS KERRAAQTTL 750
YIILDTLVRL IAPVLTFTSE EIWSYMKHDS QNNFESVQLA DWPQVQEKYN 800
NPYIIEKWEK LFDIRKDISK ALEIARTDKK IGHSLEAQVD IYPSQELYDF 850
FKGFNDLEYV FIVSKVVLHQ PEEPAPQNAY ESDDYNLKIV VTHAPGEKCE 900
RCWMYSETVG TIKEHPTICA RCASHIEQQT QV 932
Length:932
Mass (Da):107,851
Last modified:March 18, 2008 - v1
Checksum:i0BD53F63FE09E523
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000924 Genomic DNA. Translation: ABY94530.1.
RefSeqiYP_001664866.1. NC_010321.1.

Genome annotation databases

EnsemblBacteriaiABY94530; ABY94530; Teth39_0874.
GeneIDi5873648.
KEGGitpd:Teth39_0874.
PATRICi23886404. VBIThePse6203_0915.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000924 Genomic DNA. Translation: ABY94530.1 .
RefSeqi YP_001664866.1. NC_010321.1.

3D structure databases

ProteinModelPortali B0K8R7.
SMRi B0K8R7. Positions 1-928.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 340099.Teth39_0874.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABY94530 ; ABY94530 ; Teth39_0874 .
GeneIDi 5873648.
KEGGi tpd:Teth39_0874.
PATRICi 23886404. VBIThePse6203_0915.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci TPSE340099:GH4W-906-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete sequence of Thermoanaerobacter pseudethanolicus 39E."
    Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Bruce D., Goodwin L., Saunders E., Brettin T., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L.
    , Kyrpides N., Lykidis A., Hemme C., Fields M.W., He Z., Zhou J., Richardson P.
    Submitted (JAN-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 33223 / 39E.

Entry informationi

Entry nameiSYI_THEP3
AccessioniPrimary (citable) accession number: B0K8R7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 18, 2008
Last modified: May 14, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi