Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

B0K7U7 (K6PF_THEP3) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 47. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
6-phosphofructokinase

Short name=Phosphofructokinase
EC=2.7.1.11
Alternative name(s):
Phosphohexokinase
Gene names
Name:pfkA
Ordered Locus Names:Teth39_0683
OrganismThermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) (Clostridium thermohydrosulfuricum) [Complete proteome] [HAMAP]
Taxonomic identifier340099 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaThermoanaerobacteralesThermoanaerobacteraceaeThermoanaerobacter

Protein attributes

Sequence length321 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate. HAMAP-Rule MF_00339

Pathway

Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4. HAMAP-Rule MF_00339

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00339.

Sequence similarities

Belongs to the phosphofructokinase family.

Ontologies

Keywords
   Biological processGlycolysis
   Cellular componentCytoplasm
   LigandATP-binding
Magnesium
Metal-binding
Nucleotide-binding
   Molecular functionKinase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processfructose 6-phosphate metabolic process

Inferred from electronic annotation. Source: InterPro

glycolysis

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_component6-phosphofructokinase complex

Inferred from electronic annotation. Source: InterPro

   Molecular_function6-phosphofructokinase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

ATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 3213216-phosphofructokinase HAMAP-Rule MF_00339
PRO_1000120064

Regions

Nucleotide binding21 – 255ATP By similarity
Nucleotide binding155 – 1595ATP By similarity
Nucleotide binding172 – 18817ATP By similarity

Sites

Active site1281Proton acceptor By similarity
Metal binding1861Magnesium; via carbonyl oxygen By similarity
Metal binding1881Magnesium By similarity
Binding site1631Substrate By similarity
Binding site2451Substrate By similarity
Binding site2511Substrate By similarity
Binding site2541Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
B0K7U7 [UniParc].

Last modified March 18, 2008. Version 1.
Checksum: 595F90D4BF423E29

FASTA32135,215
        10         20         30         40         50         60 
MKTIGILTSG GDAPGMNAAI RAVVRTGIYY GLKVKGIMRG YAGLVEDEVI DLNLSSVGDI 

        70         80         90        100        110        120 
LQKGGTILRT ARCEEFKKKE VRKKAYETLQ KHGIEGLVVI GGDGSFRGAQ LLSEEWNVNT 

       130        140        150        160        170        180 
IGIPGTIDND IPCTDYTIGF DTACNTVIDA INKIRDTATS HERANIIEVM GRNAGYIALY 

       190        200        210        220        230        240 
AGLAGGAEMI ILPEVEWSID ELCDKITYGI KRGKLHHIIV LAEGVMSAPE LAKMIKERLP 

       250        260        270        280        290        300 
KLDLRYTILG HIQRGGAPTV MDRVLASQMG ARAVELLLEN KTKRIISIRN NQIVDDDIDE 

       310        320 
ALSMKKEFNR KLYELSKILS I 

« Hide

References

[1]"Complete sequence of Thermoanaerobacter pseudethanolicus 39E."
Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Bruce D., Goodwin L., Saunders E., Brettin T., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L. expand/collapse author list , Kyrpides N., Lykidis A., Hemme C., Fields M.W., He Z., Zhou J., Richardson P.
Submitted (JAN-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 33223 / 39E.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000924 Genomic DNA. Translation: ABY94346.1.
RefSeqYP_001664682.1. NC_010321.1.

3D structure databases

ProteinModelPortalB0K7U7.
SMRB0K7U7. Positions 1-321.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING340099.Teth39_0683.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABY94346; ABY94346; Teth39_0683.
GeneID5874001.
KEGGtpd:Teth39_0683.
PATRIC23885984. VBIThePse6203_0716.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0205.
HOGENOMHOG000248870.
KOK00850.
OMAKMVHHDI.
OrthoDBEOG644ZRM.
ProtClustDBPRK03202.

Enzyme and pathway databases

BioCycTPSE340099:GH4W-704-MONOMER.
UniPathwayUPA00109; UER00182.

Family and domain databases

HAMAPMF_00339. Phosphofructokinase.
InterProIPR012003. ATP_PFK_prok.
IPR012828. PFKA_ATP.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamPF00365. PFK. 1 hit.
[Graphical view]
PIRSFPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSPR00476. PHFRCTKINASE.
SUPFAMSSF53784. SSF53784. 1 hit.
TIGRFAMsTIGR02482. PFKA_ATP. 1 hit.
PROSITEPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameK6PF_THEP3
AccessionPrimary (citable) accession number: B0K7U7
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: March 18, 2008
Last modified: April 16, 2014
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways