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B0K6L2 (PFKA_THEPX) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 50. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
ATP-dependent 6-phosphofructokinase

Short name=ATP-PFK
Short name=Phosphofructokinase
EC=2.7.1.11
Alternative name(s):
Phosphohexokinase
Gene names
Name:pfkA
Ordered Locus Names:Teth514_1194
OrganismThermoanaerobacter sp. (strain X514) [Complete proteome] [HAMAP]
Taxonomic identifier399726 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaThermoanaerobacteralesThermoanaerobacteraceaeThermoanaerobacter

Protein attributes

Sequence length321 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis By similarity. HAMAP-Rule MF_00339

Catalytic activity

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate. HAMAP-Rule MF_00339

Cofactor

Magnesium By similarity. HAMAP-Rule MF_00339

Enzyme regulation

Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate By similarity. HAMAP-Rule MF_00339

Pathway

Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4. HAMAP-Rule MF_00339

Subunit structure

Homotetramer By similarity. HAMAP-Rule MF_00339

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00339.

Sequence similarities

Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Prokaryotic clade "B1" sub-subfamily.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 321321ATP-dependent 6-phosphofructokinase HAMAP-Rule MF_00339
PRO_1000120065

Regions

Nucleotide binding72 – 732ATP By similarity
Nucleotide binding102 – 1054ATP By similarity
Region21 – 255Allosteric activator ADP binding; shared with dimeric partner By similarity
Region126 – 1283Substrate binding By similarity
Region170 – 1723Substrate binding By similarity
Region186 – 1883Allosteric activator ADP binding By similarity
Region214 – 2163Allosteric activator ADP binding By similarity
Region251 – 2544Substrate binding By similarity

Sites

Active site1281Proton acceptor By similarity
Metal binding1031Magnesium; catalytic By similarity
Binding site111ATP; via amide nitrogen By similarity
Binding site1551Allosteric activator ADP By similarity
Binding site1631Substrate; shared with dimeric partner By similarity
Binding site2121Allosteric activator ADP By similarity
Binding site2231Substrate By similarity
Binding site2451Substrate; shared with dimeric partner By similarity

Sequences

Sequence LengthMass (Da)Tools
B0K6L2 [UniParc].

Last modified March 18, 2008. Version 1.
Checksum: 595F90D4BF423E29

FASTA32135,215
        10         20         30         40         50         60 
MKTIGILTSG GDAPGMNAAI RAVVRTGIYY GLKVKGIMRG YAGLVEDEVI DLNLSSVGDI 

        70         80         90        100        110        120 
LQKGGTILRT ARCEEFKKKE VRKKAYETLQ KHGIEGLVVI GGDGSFRGAQ LLSEEWNVNT 

       130        140        150        160        170        180 
IGIPGTIDND IPCTDYTIGF DTACNTVIDA INKIRDTATS HERANIIEVM GRNAGYIALY 

       190        200        210        220        230        240 
AGLAGGAEMI ILPEVEWSID ELCDKITYGI KRGKLHHIIV LAEGVMSAPE LAKMIKERLP 

       250        260        270        280        290        300 
KLDLRYTILG HIQRGGAPTV MDRVLASQMG ARAVELLLEN KTKRIISIRN NQIVDDDIDE 

       310        320 
ALSMKKEFNR KLYELSKILS I 

« Hide

References

[1]"Complete sequence of Thermoanaerobacter sp. X514."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Bruce D., Goodwin L., Saunders E., Brettin T., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L. expand/collapse author list , Kyrpides N., Kim E., Hemme C., Fields M.W., He Z., Zhou J., Richardson P.
Submitted (JAN-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: X514.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000923 Genomic DNA. Translation: ABY92488.1.
RefSeqYP_001662824.1. NC_010320.1.

3D structure databases

ProteinModelPortalB0K6L2.
SMRB0K6L2. Positions 1-321.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING399726.Teth514_1194.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABY92488; ABY92488; Teth514_1194.
GeneID5877678.
KEGGtex:Teth514_1194.
PATRIC23892020. VBITheSp86957_1247.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0205.
HOGENOMHOG000248870.
KOK00850.
OMAAKLERGH.
OrthoDBEOG644ZRM.

Enzyme and pathway databases

BioCycTSP399726:GHCK-1228-MONOMER.
UniPathwayUPA00109; UER00182.

Family and domain databases

HAMAPMF_00339. Phosphofructokinase_I_B1.
InterProIPR012003. ATP_PFK_prok.
IPR012828. PFKA_ATP.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamPF00365. PFK. 1 hit.
[Graphical view]
PIRSFPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSPR00476. PHFRCTKINASE.
SUPFAMSSF53784. SSF53784. 1 hit.
TIGRFAMsTIGR02482. PFKA_ATP. 1 hit.
PROSITEPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePFKA_THEPX
AccessionPrimary (citable) accession number: B0K6L2
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: March 18, 2008
Last modified: July 9, 2014
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways