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Protein

Argininosuccinate synthase

Gene

argG

Organism
Thermoanaerobacter sp. (strain X514)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate.UniRule annotation

Pathway: L-arginine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Argininosuccinate synthase (argG)
  3. Argininosuccinate lyase (argH)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei88 – 881CitrullineUniRule annotation
Binding sitei93 – 931CitrullineUniRule annotation
Binding sitei118 – 1181ATP; via amide nitrogenUniRule annotation
Binding sitei120 – 1201AspartateUniRule annotation
Binding sitei124 – 1241AspartateUniRule annotation
Binding sitei124 – 1241CitrullineUniRule annotation
Binding sitei125 – 1251AspartateUniRule annotation
Binding sitei128 – 1281CitrullineUniRule annotation
Binding sitei177 – 1771CitrullineUniRule annotation
Binding sitei186 – 1861CitrullineUniRule annotation
Binding sitei262 – 2621CitrullineUniRule annotation
Binding sitei274 – 2741CitrullineUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi10 – 189ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciTSP399726:GHCK-682-MONOMER.
UniPathwayiUPA00068; UER00113.

Names & Taxonomyi

Protein namesi
Recommended name:
Argininosuccinate synthaseUniRule annotation (EC:6.3.4.5UniRule annotation)
Alternative name(s):
Citrulline--aspartate ligaseUniRule annotation
Gene namesi
Name:argGUniRule annotation
Ordered Locus Names:Teth514_0663
OrganismiThermoanaerobacter sp. (strain X514)
Taxonomic identifieri399726 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaThermoanaerobacteralesThermoanaerobacteraceaeThermoanaerobacter
ProteomesiUP000002155 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 410410Argininosuccinate synthasePRO_1000089058Add
BLAST

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliB0K4D8.
SMRiB0K4D8. Positions 6-398.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the argininosuccinate synthase family. Type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0137.
HOGENOMiHOG000230093.
KOiK01940.
OMAiPAREWGM.
OrthoDBiEOG6K9QCV.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00005. Arg_succ_synth_type1.
InterProiIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B0K4D8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLKGEKVVLA YSGGLDTSVI IPWLKENYEC EIIAACINVG QGEELKYIKD
60 70 80 90 100
KALASGASKV YIEDVKEEFV KDYIFPTLKA GAVYEGKYLL GTSMARPLIA
110 120 130 140 150
KKLVEIAHKE GAKAIAHGAT GKGNDQVRFE VSIHALDPSI KIIAPWRIWD
160 170 180 190 200
LKSREDEIDY AKKKGIPIPV TKEKIYSVDN NLWHVSHEGG DLEDPWNEPK
210 220 230 240 250
SDLYDIITPP DKAPDKPEYV LIEFEKGIPV KVNGKALEPV KLIEELNAIA
260 270 280 290 300
GRNGVGIADL VENRLVGMKS RGVYETPAGT LLYAAHKELE YLVLDKETMR
310 320 330 340 350
FKDLVSQKYA DLVYNGLWFS PLKAALDAFV EETQKNVTGV VRLKLYKGNV
360 370 380 390 400
INAGVKSPYS LYNQEFVTFG KDEVYNQKDA EGFINLFGLS LKIKALMEME
410
RKDMDEAVGR
Length:410
Mass (Da):45,806
Last modified:March 18, 2008 - v1
Checksum:iBCDBEC1658CB4CC8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000923 Genomic DNA. Translation: ABY91971.1.
RefSeqiWP_003868142.1. NC_010320.1.

Genome annotation databases

EnsemblBacteriaiABY91971; ABY91971; Teth514_0663.
KEGGitex:Teth514_0663.
PATRICi23890879. VBITheSp86957_0693.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000923 Genomic DNA. Translation: ABY91971.1.
RefSeqiWP_003868142.1. NC_010320.1.

3D structure databases

ProteinModelPortaliB0K4D8.
SMRiB0K4D8. Positions 6-398.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABY91971; ABY91971; Teth514_0663.
KEGGitex:Teth514_0663.
PATRICi23890879. VBITheSp86957_0693.

Phylogenomic databases

eggNOGiCOG0137.
HOGENOMiHOG000230093.
KOiK01940.
OMAiPAREWGM.
OrthoDBiEOG6K9QCV.

Enzyme and pathway databases

UniPathwayiUPA00068; UER00113.
BioCyciTSP399726:GHCK-682-MONOMER.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00005. Arg_succ_synth_type1.
InterProiIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: X514.

Entry informationi

Entry nameiASSY_THEPX
AccessioniPrimary (citable) accession number: B0K4D8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: March 18, 2008
Last modified: June 24, 2015
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.