B0K403 (SYE2_THEPX) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase 2
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase 2
Short name=GluRS 2

Gene names

Name:	gltX2
Ordered Locus Names:	Teth514_2102

Organism

Thermoanaerobacter sp. (strain X514) [Complete proteome] [HAMAP]

Taxonomic identifier

399726 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Thermoanaerobacterales › Thermoanaerobacteriaceae › Thermoanaerobacter

Protein attributes

Sequence length	479 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022_B
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022_B
Subunit structure	Monomer By similarity. HAMAP MF_00022_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00022_B.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 479	479	Glutamate--tRNA ligase 2 HAMAP MF_00022_B		PRO_0000367784
Regions
	Motif	10 – 20	11	"HIGH" region HAMAP MF_00022_B
	Motif	243 – 247	5	"KMSKS" region HAMAP MF_00022_B
Sites
	Binding site	246	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	B0K403 [UniParc]. Last modified March 18, 2008. Version 1. Checksum: 237F3A29695EF085	FASTA	479	55,661
	10 20 30 40 50 60 MSEVRVRFAP SPTGSLHIGG ARTALFNWLF ARHNGGKFIL RVDDTDLQRS TEESMKGILE 70 80 90 100 110 120 GLKWLGIDWD EGPIYQSQRL EEYRKFANKL LKEGKAYYCF CTKEELEEMR KQAEKEGRPP 130 140 150 160 170 180 MYTGKCRNLT KEQIEEYLRQ GKKPVIRLKV PQQGKTVVHD IIRGDVEFDN STFDDFIIMK 190 200 210 220 230 240 SDNMPTYNFA TVVDDYQMGI THVIRAEEHL SNTPKQILIF EALGLEIPQF AHVSMVLAPD 250 260 270 280 290 300 RSKLSKRHGA TSVQEFRDQG YLPEAIVNYI TLLGWIPKDG EEIFDVSKSI KEFTLERVSK 310 320 330 340 350 360 NPAIYDVQKL TWINGHYIRN YDLDKLTEVV IPFLKAKNFI GEDFDYDYIK KIVSVVRERE 370 380 390 400 410 420 KTLVDVADAM SYYFTEVNEY EEKGVKKYFT KEKVVDILKK AVVTLKEVEP FNKFTTEETY 430 440 450 460 470 RKLVEELQIS SGELFHPTRL AISGRTFGPG LFDIMELLGK ERTIKRIEKA IDFIQKMRK

References

[1]

"Complete sequence of Thermoanaerobacter sp. X514." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Bruce D., Goodwin L., Saunders E., Brettin T., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Kim E., Hemme C., Fields M.W., He Z., Zhou J., Richardson P. Submitted (JAN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: X514.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000923 Genomic DNA. Translation: ABY93374.1.
RefSeq	YP_001663710.1. NC_010320.1.
3D structure databases
ProteinModelPortal	B0K403.
ModBase	Search...
Protein-protein interaction databases
STRING	B0K403.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5876145.
GenomeReviews	Gene locus Teth514_2102 in contig CP000923_GR.
KEGG	tex:Teth514_2102.
PATRIC	23893932. VBITheSp86957_2180.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG628189.
OMA	IEWFNLD.
ProtClustDB	PRK01406.
Enzyme and pathway databases
BioCyc	TSP1755:TETH514_2102-MONOMER.
Family and domain databases
HAMAP	MF_00022_B. Glu_tRNA_synth_B. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ib_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
KO	K01885.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit. PTHR10119:SF1. PTHR10119:SF1. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMs	TIGR00464. GltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE2_THEPX

Accession

Primary (citable) accession number: B0K403

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	March 18, 2008
Last modified:	January 25, 2012
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program