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B0K2W1

- SYI_THEPX

UniProt

B0K2W1 - SYI_THEPX

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Thermoanaerobacter sp. (strain X514)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei559 – 5591Aminoacyl-adenylateUniRule annotation
Binding sitei603 – 6031ATPUniRule annotation
Metal bindingi899 – 8991ZincUniRule annotation
Metal bindingi902 – 9021ZincUniRule annotation
Metal bindingi919 – 9191ZincUniRule annotation
Metal bindingi922 – 9221ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciTSP399726:GHCK-1932-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Teth514_1886
OrganismiThermoanaerobacter sp. (strain X514)
Taxonomic identifieri399726 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaThermoanaerobacteralesThermoanaerobacteraceaeThermoanaerobacter
ProteomesiUP000002155: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 932932Isoleucine--tRNA ligasePRO_1000189211Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi399726.Teth514_1886.

Structurei

3D structure databases

ProteinModelPortaliB0K2W1.
SMRiB0K2W1. Positions 1-928.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionAdd
BLAST
Motifi600 – 6045"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiLGRRSCQ.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B0K2W1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDYNKTLNLP RTDFPMKANL PTREPEILKR WEEMDIYHKT LEKNKGKEKY
60 70 80 90 100
ILHDGPPYAN GDIHIGTAMN KVLKDIIVKY KTMRGYDAPY VPGWDTHGLP
110 120 130 140 150
IEQQAIKTLG IKRHEVSPTE FRKVCRDFAF SQIEKQKAQF KRLGVRGDWD
160 170 180 190 200
NPYLTLNPEY EAKQIEVFGE MAKKGYIYKG LKPVYWCPSC ETALAEAEIE
210 220 230 240 250
YFDETSDSIY VKFRVKDDLG KFKGIVENLN NVYFVIWTTT TWTIPANLAI
260 270 280 290 300
ALNPEFDYAL AKFGDEVYIM AKDMLDTVKK EANLSDYEIV AVFKGKDLEG
310 320 330 340 350
MKATHPLYDR DSLIILGEHV TLEAGTGCVH TAPGHGEEDF LVGQEYGLEV
360 370 380 390 400
LNPIDDKGYF TDKAPGYAGL YYEEANKVIK EDLKKANALV AETRITHSYP
410 420 430 440 450
HCWRCKSPII FRATEQWFAS VEGFREEALK AIKEVNWYPS WGEERITNMV
460 470 480 490 500
RDRRDWCISR QRVWGVPIPI FYCEKCGKPL INDDTINAVK KIFRQKGSDA
510 520 530 540 550
WFEMSAEEIL PKGITCECGS TKFRKETDIM DVWFDSGSSH AAVLQTHPDL
560 570 580 590 600
KWPAELYLEG SDQHRGWFQS SLLTSVATRG KAPYRNVLTH GFVVDGEGRK
610 620 630 640 650
MSKSLGNGID PADVIKEYGA DILRLWTVSA DFTSDMRISQ EILKQMTEAY
660 670 680 690 700
RKIRNTSKFL LSNLYDFDPD KDMLPYEELL EIDKWALFRL NRVVEELTEA
710 720 730 740 750
FDKYEYYDFL HLVHTFCVVD MSSLYLDILK DRLYTYPATS KERRAAQTTL
760 770 780 790 800
YIILDTLVRL IAPVLTFTSE EIWSYMKHDS QNNFESVQLA DWPQVQEKYN
810 820 830 840 850
NPYIIEKWEK LFDIRKDISK ALEIARTDKK IGHSLEAQVD IYPSQELYDF
860 870 880 890 900
FKGFNDLEYV FIVSKVVLHQ PEEPAPQNAY ESDDYNLKIV VTHAPGEKCE
910 920 930
RCWMYSETVG TIKEHPTICA RCASHIEQQT QV
Length:932
Mass (Da):107,851
Last modified:March 18, 2008 - v1
Checksum:i0BD53F63FE09E523
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000923 Genomic DNA. Translation: ABY93166.1.
RefSeqiYP_001663502.1. NC_010320.1.

Genome annotation databases

EnsemblBacteriaiABY93166; ABY93166; Teth514_1886.
GeneIDi5877806.
KEGGitex:Teth514_1886.
PATRICi23893456. VBITheSp86957_1952.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000923 Genomic DNA. Translation: ABY93166.1 .
RefSeqi YP_001663502.1. NC_010320.1.

3D structure databases

ProteinModelPortali B0K2W1.
SMRi B0K2W1. Positions 1-928.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 399726.Teth514_1886.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABY93166 ; ABY93166 ; Teth514_1886 .
GeneIDi 5877806.
KEGGi tex:Teth514_1886.
PATRICi 23893456. VBITheSp86957_1952.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi LGRRSCQ.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci TSP399726:GHCK-1932-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: X514.

Entry informationi

Entry nameiSYI_THEPX
AccessioniPrimary (citable) accession number: B0K2W1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 18, 2008
Last modified: October 29, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3