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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Thermoanaerobacter sp. (strain X514)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei559Aminoacyl-adenylateUniRule annotation1
Binding sitei603ATPUniRule annotation1
Metal bindingi899ZincUniRule annotation1
Metal bindingi902ZincUniRule annotation1
Metal bindingi919ZincUniRule annotation1
Metal bindingi922ZincUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Teth514_1886
OrganismiThermoanaerobacter sp. (strain X514)
Taxonomic identifieri399726 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaThermoanaerobacteralesThermoanaerobacteraceaeThermoanaerobacter
Proteomesi
  • UP000002155 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001892111 – 932Isoleucine--tRNA ligaseAdd BLAST932

Interactioni

Subunit structurei

Monomer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliB0K2W1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi57 – 67"HIGH" regionAdd BLAST11
Motifi600 – 604"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000246402.
KOiK01870.
OMAiTHGFIVD.
OrthoDBiPOG091H028I.

Family and domain databases

CDDicd07960. Anticodon_Ia_Ile_BEm. 1 hit.
Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR033708. Anticodon_Ile_BEm.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_Ia_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_FPG/IleRS.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B0K2W1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDYNKTLNLP RTDFPMKANL PTREPEILKR WEEMDIYHKT LEKNKGKEKY
60 70 80 90 100
ILHDGPPYAN GDIHIGTAMN KVLKDIIVKY KTMRGYDAPY VPGWDTHGLP
110 120 130 140 150
IEQQAIKTLG IKRHEVSPTE FRKVCRDFAF SQIEKQKAQF KRLGVRGDWD
160 170 180 190 200
NPYLTLNPEY EAKQIEVFGE MAKKGYIYKG LKPVYWCPSC ETALAEAEIE
210 220 230 240 250
YFDETSDSIY VKFRVKDDLG KFKGIVENLN NVYFVIWTTT TWTIPANLAI
260 270 280 290 300
ALNPEFDYAL AKFGDEVYIM AKDMLDTVKK EANLSDYEIV AVFKGKDLEG
310 320 330 340 350
MKATHPLYDR DSLIILGEHV TLEAGTGCVH TAPGHGEEDF LVGQEYGLEV
360 370 380 390 400
LNPIDDKGYF TDKAPGYAGL YYEEANKVIK EDLKKANALV AETRITHSYP
410 420 430 440 450
HCWRCKSPII FRATEQWFAS VEGFREEALK AIKEVNWYPS WGEERITNMV
460 470 480 490 500
RDRRDWCISR QRVWGVPIPI FYCEKCGKPL INDDTINAVK KIFRQKGSDA
510 520 530 540 550
WFEMSAEEIL PKGITCECGS TKFRKETDIM DVWFDSGSSH AAVLQTHPDL
560 570 580 590 600
KWPAELYLEG SDQHRGWFQS SLLTSVATRG KAPYRNVLTH GFVVDGEGRK
610 620 630 640 650
MSKSLGNGID PADVIKEYGA DILRLWTVSA DFTSDMRISQ EILKQMTEAY
660 670 680 690 700
RKIRNTSKFL LSNLYDFDPD KDMLPYEELL EIDKWALFRL NRVVEELTEA
710 720 730 740 750
FDKYEYYDFL HLVHTFCVVD MSSLYLDILK DRLYTYPATS KERRAAQTTL
760 770 780 790 800
YIILDTLVRL IAPVLTFTSE EIWSYMKHDS QNNFESVQLA DWPQVQEKYN
810 820 830 840 850
NPYIIEKWEK LFDIRKDISK ALEIARTDKK IGHSLEAQVD IYPSQELYDF
860 870 880 890 900
FKGFNDLEYV FIVSKVVLHQ PEEPAPQNAY ESDDYNLKIV VTHAPGEKCE
910 920 930
RCWMYSETVG TIKEHPTICA RCASHIEQQT QV
Length:932
Mass (Da):107,851
Last modified:March 18, 2008 - v1
Checksum:i0BD53F63FE09E523
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000923 Genomic DNA. Translation: ABY93166.1.
RefSeqiWP_009052491.1. NC_010320.1.

Genome annotation databases

EnsemblBacteriaiABY93166; ABY93166; Teth514_1886.
KEGGitex:Teth514_1886.
PATRICi23893456. VBITheSp86957_1952.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000923 Genomic DNA. Translation: ABY93166.1.
RefSeqiWP_009052491.1. NC_010320.1.

3D structure databases

ProteinModelPortaliB0K2W1.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABY93166; ABY93166; Teth514_1886.
KEGGitex:Teth514_1886.
PATRICi23893456. VBITheSp86957_1952.

Phylogenomic databases

HOGENOMiHOG000246402.
KOiK01870.
OMAiTHGFIVD.
OrthoDBiPOG091H028I.

Family and domain databases

CDDicd07960. Anticodon_Ia_Ile_BEm. 1 hit.
Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR033708. Anticodon_Ile_BEm.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_Ia_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_FPG/IleRS.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYI_THEPX
AccessioniPrimary (citable) accession number: B0K2W1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 18, 2008
Last modified: November 2, 2016
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.