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Protein

NAD kinase

Gene

nadK

Organism
Thermoanaerobacter sp. (strain X514)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP.UniRule annotation

Catalytic activityi

ATP + NAD+ = ADP + NADP+.UniRule annotation

Cofactori

a divalent metal cationUniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei68 – 681Proton acceptorUniRule annotation
Binding sitei153 – 1531NADUniRule annotation
Binding sitei172 – 1721NADUniRule annotation
Binding sitei242 – 2421NADUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi68 – 692NADUniRule annotation
Nucleotide bindingi142 – 1432NADUniRule annotation
Nucleotide bindingi183 – 1886NADUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-HAMAP
  3. NAD+ kinase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. NAD metabolic process Source: InterPro
  2. NADP biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, NAD, NADP, Nucleotide-binding

Enzyme and pathway databases

BioCyciTSP399726:GHCK-1581-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
NAD kinaseUniRule annotation (EC:2.7.1.23UniRule annotation)
Alternative name(s):
ATP-dependent NAD kinaseUniRule annotation
Gene namesi
Name:nadKUniRule annotation
Ordered Locus Names:Teth514_1542
OrganismiThermoanaerobacter sp. (strain X514)
Taxonomic identifieri399726 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaThermoanaerobacteralesThermoanaerobacteraceaeThermoanaerobacter
ProteomesiUP000002155: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 283283NAD kinasePRO_1000120895Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi399726.Teth514_1542.

Structurei

3D structure databases

ProteinModelPortaliB0K0V4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NAD kinase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0061.
HOGENOMiHOG000227222.
KOiK00858.
OMAiVITPECP.
OrthoDBiEOG6PZXDR.

Family and domain databases

Gene3Di2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPiMF_00361. NAD_kinase.
InterProiIPR017438. ATP-NAD_kinase_dom_1.
IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR002504. PolyP/ATP_NADK.
[Graphical view]
PANTHERiPTHR20275. PTHR20275. 1 hit.
PfamiPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.

Sequencei

Sequence statusi: Complete.

B0K0V4-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKKVGVIPNI NKDKDLEVTK SVVKWLLEHD SEPYLNEIVA SKMGYDEYGK
60 70 80 90 100
KSTDIYSKSD FIIALGGDGT ILNVARLCAP FGTPIFAVNL GHLGFLTEVD
110 120 130 140 150
MNEVFVSLDK IYKGEYTVEK RMMLEANVVK NDMEIINFRA LNDIVITRGA
160 170 180 190 200
FSRMARINAY VNNNYVDTYL ADGVIIATPT GSTAYSLSAG GPIVYPTVEV
210 220 230 240 250
IIITPICPHT LYSRSIIVSP DDVIRLEISE ENQDLMITTD GQQGYKLDYR
260 270 280
DIIYIKKSNE YTNLIRVKNT NFFDLLRDKL TER
Length:283
Mass (Da):31,908
Last modified:March 18, 2008 - v1
Checksum:iDCC8D88EB7EEE705
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000923 Genomic DNA. Translation: ABY92829.1.
RefSeqiYP_001663165.1. NC_010320.1.

Genome annotation databases

EnsemblBacteriaiABY92829; ABY92829; Teth514_1542.
GeneIDi5876728.
KEGGitex:Teth514_1542.
PATRICi23892738. VBITheSp86957_1600.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000923 Genomic DNA. Translation: ABY92829.1.
RefSeqiYP_001663165.1. NC_010320.1.

3D structure databases

ProteinModelPortaliB0K0V4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi399726.Teth514_1542.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABY92829; ABY92829; Teth514_1542.
GeneIDi5876728.
KEGGitex:Teth514_1542.
PATRICi23892738. VBITheSp86957_1600.

Phylogenomic databases

eggNOGiCOG0061.
HOGENOMiHOG000227222.
KOiK00858.
OMAiVITPECP.
OrthoDBiEOG6PZXDR.

Enzyme and pathway databases

BioCyciTSP399726:GHCK-1581-MONOMER.

Family and domain databases

Gene3Di2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPiMF_00361. NAD_kinase.
InterProiIPR017438. ATP-NAD_kinase_dom_1.
IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR002504. PolyP/ATP_NADK.
[Graphical view]
PANTHERiPTHR20275. PTHR20275. 1 hit.
PfamiPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: X514.

Entry informationi

Entry nameiNADK_THEPX
AccessioniPrimary (citable) accession number: B0K0V4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: March 18, 2008
Last modified: January 7, 2015
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.