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Protein

CDGSH iron-sulfur domain-containing protein 1

Gene

Cisd1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Plays a key role in regulating maximal capacity for electron transport and oxidative phosphorylation. May be involved in Fe-S cluster shuttling and/or in redox reactions.By similarity

Cofactori

[2Fe-2S] clusterBy similarityNote: Binds 1 [2Fe-2S] cluster per subunit. The [2Fe-2S] cluster is redox-active and pH labile and is significantly less stable at pH 4.5 as compared with pH 7.0.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi72Iron-sulfur (2Fe-2S)By similarity1
Metal bindingi74Iron-sulfur (2Fe-2S)By similarity1
Metal bindingi83Iron-sulfur (2Fe-2S)By similarity1
Metal bindingi87Iron-sulfur (2Fe-2S); via pros nitrogenBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Ligand2Fe-2S, Iron, Iron-sulfur, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
CDGSH iron-sulfur domain-containing protein 1
Alternative name(s):
MitoNEET
Gene namesi
Name:Cisd1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 20

Organism-specific databases

RGDi1309529. Cisd1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei13 – 31Helical; Signal-anchor for type III membrane proteinSequence analysisAdd BLAST19
Topological domaini32 – 108CytoplasmicSequence analysisAdd BLAST77

GO - Cellular componenti

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion outer membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00003547052 – 108CDGSH iron-sulfur domain-containing protein 1Add BLAST107

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki42Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residuei55N6-acetyllysine; alternateBy similarity1
Cross-linki55Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternateBy similarity
Modified residuei68N6-acetyllysine; alternateBy similarity1
Cross-linki68Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternateBy similarity
Cross-linki78Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Cross-linki79Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Cross-linki89Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residuei104N6-acetyllysine; alternateBy similarity1
Cross-linki104Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternateBy similarity
Cross-linki105Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Cross-linki106Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity

Post-translational modificationi

Ubiquitinated by PARK2 during mitophagy, leading to its degradation and enhancement of mitophagy. Deubiquitinated by USP30.By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbiB0K020.
PeptideAtlasiB0K020.
PRIDEiB0K020.

PTM databases

iPTMnetiB0K020.
PhosphoSitePlusiB0K020.
SwissPalmiB0K020.

Expressioni

Gene expression databases

BgeeiENSRNOG00000000610.
GenevisibleiB0K020. RN.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000000749.

Structurei

3D structure databases

ProteinModelPortaliB0K020.
SMRiB0K020.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the CISD protein family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3461. Eukaryota.
ENOG41122II. LUCA.
GeneTreeiENSGT00390000001233.
HOGENOMiHOG000242301.
HOVERGENiHBG052444.
InParanoidiB0K020.
OMAiKDHRNKS.
OrthoDBiEOG091G0XB4.
PhylomeDBiB0K020.
TreeFamiTF324661.

Family and domain databases

InterProiView protein in InterPro
IPR018967. FeS-contain_CDGSH-typ.
IPR019610. FeS-contain_mitoNEET_N.
PfamiView protein in Pfam
PF10660. MitoNEET_N. 1 hit.
PF09360. zf-CDGSH. 1 hit.
SMARTiView protein in SMART
SM00704. ZnF_CDGSH. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

B0K020-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGLSSDSPVR VEWIAAVTFA AGTAALGYLA YKKFYAKESR TKAMVNLQIQ
60 70 80 90 100
KDNPKVVHAF DMEDLGDKAV YCRCWRSKKF PFCDGAHIKH NEETGDNVGP

LIIKKKET
Length:108
Mass (Da):12,097
Last modified:March 18, 2008 - v1
Checksum:i72B2548F490E5FA3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH473988 Genomic DNA. Translation: EDL97255.1.
BC159419 mRNA. Translation: AAI59420.1.
RefSeqiNP_001099855.1. NM_001106385.2.
UniGeneiRn.1164.

Genome annotation databases

EnsembliENSRNOT00000000749; ENSRNOP00000000749; ENSRNOG00000000610.
GeneIDi294362.
KEGGirno:294362.
UCSCiRGD:1309529. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH473988 Genomic DNA. Translation: EDL97255.1.
BC159419 mRNA. Translation: AAI59420.1.
RefSeqiNP_001099855.1. NM_001106385.2.
UniGeneiRn.1164.

3D structure databases

ProteinModelPortaliB0K020.
SMRiB0K020.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000000749.

PTM databases

iPTMnetiB0K020.
PhosphoSitePlusiB0K020.
SwissPalmiB0K020.

Proteomic databases

PaxDbiB0K020.
PeptideAtlasiB0K020.
PRIDEiB0K020.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000000749; ENSRNOP00000000749; ENSRNOG00000000610.
GeneIDi294362.
KEGGirno:294362.
UCSCiRGD:1309529. rat.

Organism-specific databases

CTDi55847.
RGDi1309529. Cisd1.

Phylogenomic databases

eggNOGiKOG3461. Eukaryota.
ENOG41122II. LUCA.
GeneTreeiENSGT00390000001233.
HOGENOMiHOG000242301.
HOVERGENiHBG052444.
InParanoidiB0K020.
OMAiKDHRNKS.
OrthoDBiEOG091G0XB4.
PhylomeDBiB0K020.
TreeFamiTF324661.

Miscellaneous databases

PROiPR:B0K020.

Gene expression databases

BgeeiENSRNOG00000000610.
GenevisibleiB0K020. RN.

Family and domain databases

InterProiView protein in InterPro
IPR018967. FeS-contain_CDGSH-typ.
IPR019610. FeS-contain_mitoNEET_N.
PfamiView protein in Pfam
PF10660. MitoNEET_N. 1 hit.
PF09360. zf-CDGSH. 1 hit.
SMARTiView protein in SMART
SM00704. ZnF_CDGSH. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCISD1_RAT
AccessioniPrimary (citable) accession number: B0K020
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 25, 2008
Last sequence update: March 18, 2008
Last modified: March 15, 2017
This is version 64 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.