B0K019 (BAG1_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
BAG family molecular chaperone regulator 1
Short name=BAG-1

Alternative name(s):
Bcl-2-associated athanogene 1

Gene names

Name:

Bag1

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	358 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release. Inhibits the pro-apoptotic function of PPP1R15A, and has anti-apoptotic activity. Markedly increases the anti-cell death function of BCL2 induced by various stimuli By similarity.
Subunit structure	Homodimer. Forms a heteromeric complex with HSP70/HSC70. Binds to the ATPase domain of HSP/HSC70 chaperones. Interacts with NR3C1. Interacts with the N-terminal region of STK19. Interacts with PPP1R15A. Interacts with BCL2 in an ATP-dependent manner By similarity.
Subcellular location	Nucleus By similarity. Cytoplasm By similarity.
Post-translational modification	Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation By similarity.
Sequence similarities	Contains 1 BAG domain. Contains 1 ubiquitin-like domain.

Ontologies

Keywords
Biological process	Apoptosis
Cellular component	Cytoplasm Nucleus
Domain	Repeat
Molecular function	Chaperone
PTM	Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	apoptotic process Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of apoptotic process Inferred from mutant phenotype. Source: RGD response to drug Inferred from expression pattern. Source: RGD response to lithium ion Inferred from expression pattern. Source: RGD response to nicotine Inferred from expression pattern. Source: RGD response to stress Inferred from expression pattern. Source: RGD
Cellular component	cytosol Inferred from direct assay. Source: RGD mitochondrion Inferred from direct assay. Source: RGD nucleus Inferred from direct assay. Source: RGD perinuclear region of cytoplasm Inferred from direct assay. Source: RGD
Molecular function	chaperone binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 358	358	BAG family molecular chaperone regulator 1		PRO_0000389100
Regions
	Repeat	102 – 111	10	1
	Repeat	114 – 119	6	2
	Repeat	120 – 125	6	3
	Repeat	126 – 131	6	4
	Repeat	132 – 137	6	5
	Repeat	144 – 149	6	6
	Repeat	150 – 155	6	7
	Domain	157 – 237	81	Ubiquitin-like
	Domain	259 – 339	81	BAG
	Region	114 – 212	99	7 X 6 AA tandem repeat of E-E-X(4)
	Region	185 – 232	48	Interaction with HSPA8 By similarity
	Region	229 – 358	130	Interaction with PPP1R15A By similarity

Sequences

	Sequence		Length	Mass (Da)
	B0K019 [UniParc]. Last modified March 18, 2008. Version 1. Checksum: 7C39247930E3E198	FASTA	358	40,160
	10 20 30 40 50 60 MADRGGARRP RGDQEPLGPR LRAPRSARET RQSESRAERG LPPSQRSSVR SAASGHDRST 70 80 90 100 110 120 RGAASGACKP RVKKKVRPRS SQSEKVAHSK ELTRSKKLTR SKKVTGTQEA TQVEEVTTIE 130 140 150 160 170 180 EATQTEEITV AEEVTQTENM AQTEEMVQTE EMEPPTLSVV VTHSNERYDL LVTPQQGNSE 190 200 210 220 230 240 PIVQDLAQLV EEATGVPLPF QKLIFKGKSL KEMETPLSAL GMQNGCRVML IGEKSNPEEE 250 260 270 280 290 300 AELKKLKDLE VSVEKTANHL EELNKELSDI QQGFLAKELQ AEALCRLDRK IKATIEQFMK 310 320 330 340 350 ILEEIDTMVL PENFKDSRLK RKNLVKKVQV FLAECDTVEQ YICQETERLQ STNLALPE

References

[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC159418 mRNA. Translation: AAI59419.1.
IPI	IPI00869713.
RefSeq	NP_001100117.2. NM_001106647.2.
UniGene	Rn.16320.
3D structure databases
ProteinModelPortal	B0K019.
SMR	B0K019. Positions 157-358.
ModBase	Search...
Protein-protein interaction databases
STRING	B0K019.
Proteomic databases
PRIDE	B0K019.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	297994.
KEGG	rno:297994.
Organism-specific databases
CTD	573.
RGD	1305203. Bag1.
Phylogenomic databases
eggNOG	roNOG16479.
GeneTree	ENSGT00450000040296.
HOVERGEN	HBG000236.
OrthoDB	EOG4X6C9F.
Family and domain databases
InterPro	IPR003103. BAG_domain. IPR017093. Molecular_chp_reg_BAG_1. IPR000626. Ubiquitin. IPR019955. Ubiquitin_supergroup. [Graphical view]
KO	K09555.
Pfam	PF02179. BAG. 1 hit. PF00240. ubiquitin. 1 hit. [Graphical view]
PIRSF	PIRSF037029. BAG_1. 1 hit.
SMART	SM00264. BAG. 1 hit. SM00213. UBQ. 1 hit. [Graphical view]
PROSITE	PS51035. BAG. 1 hit. PS00299. UBIQUITIN_1. False negative. PS50053. UBIQUITIN_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	642929.

Entry information

Entry name

BAG1_RAT

Accession

Primary (citable) accession number: B0K019

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 24, 2009
Last sequence update:	March 18, 2008
Last modified:	December 14, 2011
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program