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Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Microcystis aeruginosa (strain NIES-843)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi496 – 4961MagnesiumUniRule annotation
Metal bindingi502 – 5021MagnesiumUniRule annotation

GO - Molecular functioni

  1. 3'-5'-exoribonuclease activity Source: InterPro
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. polyribonucleotide nucleotidyltransferase activity Source: UniProtKB-HAMAP
  4. RNA binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. mRNA catabolic process Source: UniProtKB-HAMAP
  2. RNA processing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding

Enzyme and pathway databases

BioCyciMAER449447:GHO8-5382-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
Alternative name(s):
Polynucleotide phosphorylaseUniRule annotation
Short name:
PNPaseUniRule annotation
Gene namesi
Name:pnpUniRule annotation
Ordered Locus Names:MAE_53390
OrganismiMicrocystis aeruginosa (strain NIES-843)
Taxonomic identifieri449447 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesMicrocystis
ProteomesiUP000001510: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 717717Polyribonucleotide nucleotidyltransferasePRO_1000087992Add
BLAST

Proteomic databases

PaxDbiB0JYC3.
PRIDEiB0JYC3.

Interactioni

Protein-protein interaction databases

STRINGi449447.MAE_53390.

Structurei

3D structure databases

ProteinModelPortaliB0JYC3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini563 – 62260KHUniRule annotationAdd
BLAST
Domaini632 – 70069S1 motifUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation
Contains 1 KH domain.UniRule annotation
Contains 1 S1 motif domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218326.
KOiK00962.
OMAiRFMFHYN.
OrthoDBiEOG6WT8CC.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B0JYC3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEEFDKSISF DGRDIRLKIG LLAPQAGGSV LIQSGETAVL VTATTAKARE
60 70 80 90 100
GVDFLPLTVD YEERLYAAGR IPGGFLRREG RPPEKAILTG RLIDRPLRPL
110 120 130 140 150
FPNWMRDDIQ VVATTLSMDE EVPPDVLAVT GASVAVLLAG IPFQGPMAAV
160 170 180 190 200
RVGLLGDEFI INPTYKEIHN GELDLVVAGS PEGVVMIEAG ANQLPERDII
210 220 230 240 250
EAIDFGYEAV RDLITAQREL IDQLGIPLVT REAPAVNETV QNFLREQAKE
260 270 280 290 300
EIKQILSQFT LSKTERDEKL EAIENRIKET ITALPDEDTL KAPTLEEPKL
310 320 330 340 350
IGNLFKDLTK KLMRAQIIED GVRVDGRQLD EVRPISCRVG VLPRRVHGSG
360 370 380 390 400
LFNRGLTQVL SIATLGTPGD AQDLGDDLHP EDEKRYLHHY NFPPFSVGET
410 420 430 440 450
RPMRSPGRRE IGHGALAERA IVPVLPPQEE FPYVLRVVSE VLSSNGSTSM
460 470 480 490 500
GSVCGSTLAL MDAGVPLKKP VSGAAMGLIK EGEEVRILTD IQGIEDFLGD
510 520 530 540 550
MDFKVAGTDS GITALQMDMK IPGLSMEVVA KAIEQALPAR KHILEKMLAT
560 570 580 590 600
LEKPRTELSP HAPRLLTIKI DPDLIGLVIG PGGKTVKGIT EQTGTKIDID
610 620 630 640 650
DDGTVTISST DGEQAEKAKR LIYNMTRKLN EGEVYLGRVT RIIQIGAFVE
660 670 680 690 700
VLPGKEGMIH ISQLAEGRVG KVEDEVAVGD EVLVKVREID SKGRLNLTRL
710
GIHPDEAAAA RKAATVV
Length:717
Mass (Da):78,010
Last modified:March 18, 2008 - v1
Checksum:iA889548D74795D26
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP009552 Genomic DNA. Translation: BAG05161.1.
RefSeqiWP_012267692.1. NC_010296.1.
YP_001660353.1. NC_010296.1.

Genome annotation databases

EnsemblBacteriaiBAG05161; BAG05161; MAE_53390.
GeneIDi5866178.
KEGGimar:MAE_53390.
PATRICi22635127. VBIMicAer59304_4866.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP009552 Genomic DNA. Translation: BAG05161.1.
RefSeqiWP_012267692.1. NC_010296.1.
YP_001660353.1. NC_010296.1.

3D structure databases

ProteinModelPortaliB0JYC3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi449447.MAE_53390.

Proteomic databases

PaxDbiB0JYC3.
PRIDEiB0JYC3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAG05161; BAG05161; MAE_53390.
GeneIDi5866178.
KEGGimar:MAE_53390.
PATRICi22635127. VBIMicAer59304_4866.

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218326.
KOiK00962.
OMAiRFMFHYN.
OrthoDBiEOG6WT8CC.

Enzyme and pathway databases

BioCyciMAER449447:GHO8-5382-MONOMER.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NIES-843.

Entry informationi

Entry nameiPNP_MICAN
AccessioniPrimary (citable) accession number: B0JYC3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: March 18, 2008
Last modified: March 4, 2015
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.