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Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Microcystis aeruginosa (strain NIES-843)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei253 – 2531ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. glutamate-tRNA ligase activity Source: UniProtKB-HAMAP
  3. tRNA binding Source: InterPro

GO - Biological processi

  1. glutamyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMAER449447:GHO8-5310-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetaseUniRule annotation
Short name:
GluRSUniRule annotation
Gene namesi
Name:gltXUniRule annotation
Ordered Locus Names:MAE_52690
OrganismiMicrocystis aeruginosa (strain NIES-843)
Taxonomic identifieri449447 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesMicrocystis
ProteomesiUP000001510 Componenti: Chromosome

Subcellular locationi

  1. Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 480480Glutamate--tRNA ligasePRO_1000074325Add
BLAST

Proteomic databases

PaxDbiB0JY53.
PRIDEiB0JY53.

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi449447.MAE_52690.

Structurei

3D structure databases

ProteinModelPortaliB0JY53.
SMRiB0JY53. Positions 2-478.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi9 – 1911"HIGH" regionAdd
BLAST
Motifi250 – 2545"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0008.
HOGENOMiHOG000252722.
KOiK01885.
OMAiKLNWINS.
OrthoDBiEOG6DRPF7.

Family and domain databases

Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B0JY53-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTVRVRIAPS PTGNLHIGTA RTAVFNWLFA RHHRGKFILR VEDTDLERSR
60 70 80 90 100
PEYTENIQAG LQWLGLNWDE GPFFQTQRLN YYRQAIQTLL DRGLAYRCYC
110 120 130 140 150
TPEELEKMRE EQKARNLAPR YDNRHRYLTP EQQAQFEQAG RKAVIRFIID
160 170 180 190 200
DDQEIIWQDL IREKVIWKGS DLGGDMVIAR TSENGEENFG QPLYNLAVVV
210 220 230 240 250
DDIDMEITHV IRGEDHIANT AKQILLYEAL GAKVPEFAHS PLILNQEGRK
260 270 280 290 300
LSKRDGVTSI DDFRKLGFLP QALVNYMTLL GWTPPDSTEE IFTLEAAAEV
310 320 330 340 350
FSLERVNKAG AKFDWTKLDW INSQYLHRLT GEELVPLLLP YWQEAGYNFA
360 370 380 390 400
AETDRAWLIG LATLIGPSLT RLSDAVAESR LLLTPLANYN QEALSQLQLE
410 420 430 440 450
GVKDIIKDIL AAITPDLTGE VAKGIVETTT KAHRVKKGLV MKSLRAALMG
460 470 480
ELHGPDLMQS WLLLNQKGWD ISRLQQAVNS
Length:480
Mass (Da):54,663
Last modified:March 18, 2008 - v1
Checksum:i0A7A5D456C43761D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP009552 Genomic DNA. Translation: BAG05091.1.
RefSeqiWP_012267638.1. NC_010296.1.
YP_001660283.1. NC_010296.1.

Genome annotation databases

EnsemblBacteriaiBAG05091; BAG05091; MAE_52690.
GeneIDi5866106.
KEGGimar:MAE_52690.
PATRICi22634997. VBIMicAer59304_4802.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP009552 Genomic DNA. Translation: BAG05091.1.
RefSeqiWP_012267638.1. NC_010296.1.
YP_001660283.1. NC_010296.1.

3D structure databases

ProteinModelPortaliB0JY53.
SMRiB0JY53. Positions 2-478.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi449447.MAE_52690.

Proteomic databases

PaxDbiB0JY53.
PRIDEiB0JY53.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAG05091; BAG05091; MAE_52690.
GeneIDi5866106.
KEGGimar:MAE_52690.
PATRICi22634997. VBIMicAer59304_4802.

Phylogenomic databases

eggNOGiCOG0008.
HOGENOMiHOG000252722.
KOiK01885.
OMAiKLNWINS.
OrthoDBiEOG6DRPF7.

Enzyme and pathway databases

BioCyciMAER449447:GHO8-5310-MONOMER.

Family and domain databases

Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NIES-843.

Entry informationi

Entry nameiSYE_MICAN
AccessioniPrimary (citable) accession number: B0JY53
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: March 18, 2008
Last modified: April 29, 2015
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.