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B0JX07 (BIOD_MICAN) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 28. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
ATP-dependent dethiobiotin synthetase BioD
EC=6.3.3.3
Alternative name(s):
DTB synthetase
Short name=DTBS
Dethiobiotin synthase
Gene names
Name:bioD
Ordered Locus Names:MAE_50720
OrganismMicrocystis aeruginosa (strain NIES-843) [Complete proteome] [HAMAP]
Taxonomic identifier449447 [NCBI]
Taxonomic lineageBacteria › Cyanobacteria › Chroococcales › Microcystis

Protein attributes

Sequence length224 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes a mechanistically unusual reaction, the ATP-dependent insertion of CO2 between the N7 and N8 nitrogen atoms of 7,8-diaminopelargonic acid (DAPA) to form an ureido ring By similarity. HAMAP MF_00336

Catalytic activity

ATP + 7,8-diaminononanoate + CO2 = ADP + phosphate + dethiobiotin. HAMAP MF_00336

Cofactor

Magnesium By similarity. HAMAP MF_00336

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 1/2. HAMAP MF_00336

Subcellular location

Cytoplasm By similarity HAMAP MF_00336.

Sequence similarities

Belongs to the dethiobiotin synthetase family.

Ontologies

Keywords
   Biological processBiotin biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Magnesium
Metal-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processbiotin biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

dethiobiotin synthase activity

Inferred from electronic annotation. Source: EC

magnesium ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 224224ATP-dependent dethiobiotin synthetase BioD HAMAP MF_00336
PRO_1000079275

Regions

Nucleotide binding12 – 176ATP By similarity
Nucleotide binding106 – 1094ATP By similarity
Nucleotide binding166 – 1672ATP By similarity
Nucleotide binding198 – 2003ATP By similarity

Sites

Metal binding121Magnesium 1 By similarity
Metal binding161Magnesium 2 By similarity
Metal binding471Magnesium 2 By similarity
Metal binding1061Magnesium 2 By similarity
Binding site431Substrate By similarity
Binding site471ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
B0JX07 [UniParc].

Last modified March 18, 2008. Version 1.
Checksum: 5D8B8A78517B3BEF

FASTA22424,417
        10         20         30         40         50         60 
MNSLLIAATD TDAGKTVVTT GLVAYWQKYY PSKALGLMKL MQTGQGDREW YEGLFQGQLE 

        70         80         90        100        110        120 
MITPLQYQAP LAPPVAADLE GRDIPLGTVW QALLNLQKSQ DLVLIEGLGG LGCPVTHELT 

       130        140        150        160        170        180 
LADLAAQWRL KTLLVVPVKL GAISQTVANI ALAEQKKVNL GGIILNCLEP RTETEIEQLT 

       190        200        210        220 
PIDLIQSLTN CPVLGVFPFI EDRRDLDKLA SVVASWPEIK LSSI

« Hide

References

[1]"Complete genomic structure of the bloom-forming toxic cyanobacterium Microcystis aeruginosa NIES-843."
Kaneko T., Nakajima N., Okamoto S., Suzuki I., Tanabe Y., Tamaoki M., Nakamura Y., Kasai F., Watanabe A., Kawashima K., Kishida Y., Ono A., Shimizu Y., Takahashi C., Minami C., Fujishiro T., Kohara M., Katoh M. expand/collapse author list , Nakazaki N., Nakayama S., Yamada M., Tabata S., Watanabe M.M.
DNA Res. 14:247-256(2007) [PubMed: 18192279] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: NIES-843.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AP009552 Genomic DNA. Translation: BAG04894.1.
RefSeqYP_001660086.1. NC_010296.1.

3D structure databases

ProteinModelPortalB0JX07.
ModBaseSearch...

Protein-protein interaction databases

STRINGB0JX07.

Proteomic databases

PRIDEB0JX07.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID5865909.
GenomeReviewsGene locus MAE_50720 in contig AP009552_GR.
KEGGmar:MAE_50720.
PATRIC22634619. VBIMicAer59304_4613.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG650065.
OMAALAAYWQ.
PhylomeDBB0JX07.
ProtClustDBPRK00090.

Enzyme and pathway databases

BioCycMAER449447:MAE_50720-MONOMER.

Family and domain databases

HAMAPMF_00336. BioD.
[Tree]
InterProIPR002586. CbiA_P_synth.
IPR004472. DTB_synth_BioD.
[Graphical view]
KOK01935.
PfamPF01656. CbiA. 1 hit.
[Graphical view]
PIRSFPIRSF006755. DTB_synth. 1 hit.
TIGRFAMsTIGR00347. BioD. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOD_MICAN
AccessionPrimary (citable) accession number: B0JX07
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: March 18, 2008
Last modified: January 25, 2012
This is version 28 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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