B0JUF7 (ISPD_MICAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
EC=2.7.7.60

Alternative name(s):
4-diphosphocytidyl-2C-methyl-D-erythritol synthase
MEP cytidylyltransferase
Short name=MCT

Gene names

Name:	ispD
Ordered Locus Names:	MAE_45830

Organism

Microcystis aeruginosa (strain NIES-843) [Complete proteome] [HAMAP]

Taxonomic identifier

449447 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Microcystis

Protein attributes

Sequence length	226 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP) By similarity. HAMAP MF_00108
Catalytic activity	CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00108
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 2/6. HAMAP MF_00108
Sequence similarities	Belongs to the IspD family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoprenoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 226	226	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase HAMAP MF_00108		PRO_1000075935
Sites
	Site	13	1	Transition state stabilizer By similarity
	Site	20	1	Transition state stabilizer By similarity
	Site	152	1	Positions MEP for the nucleophilic attack By similarity
	Site	208	1	Positions MEP for the nucleophilic attack By similarity

Sequences

	Sequence		Length	Mass (Da)
	B0JUF7 [UniParc]. Last modified March 18, 2008. Version 1. Checksum: 0CA7616B81EB68BC	FASTA	226	24,856
	10 20 30 40 50 60 MYLLIPAAGM GKRMGSDRNK LLLTLHGKPL LAWTLLAAME SRAIIWIGIM AQPEDFEEIR 70 80 90 100 110 120 AIITPINALK PVQIIQGGET RQKSVYNGLQ ALPEGAETVL IHDGARCLAT PELFDRCAAA 130 140 150 160 170 180 LATCQGFIAA IPVKDTIKIV DSKGWIEDTP DRSRLWAAQT PQGFQVKLLK ECHEQGRKLD 190 200 210 220 WEVTDDAALL EKCGFPVKIV VGEETNLKIT TPGDLAIAEY ILSQRL

References

[1]

"Complete genomic structure of the bloom-forming toxic cyanobacterium Microcystis aeruginosa NIES-843." Kaneko T., Nakajima N., Okamoto S., Suzuki I., Tanabe Y., Tamaoki M., Nakamura Y., Kasai F., Watanabe A., Kawashima K., Kishida Y., Ono A., Shimizu Y., Takahashi C., Minami C., Fujishiro T., Kohara M., Katoh M. , Nakazaki N., Nakayama S., Yamada M., Tabata S., Watanabe M.M. DNA Res. 14:247-256(2007) [PubMed: 18192279] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NIES-843.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP009552 Genomic DNA. Translation: BAG04405.1.
RefSeq	YP_001659597.1. NC_010296.1.
3D structure databases
ProteinModelPortal	B0JUF7.
ModBase	Search...
Protein-protein interaction databases
STRING	B0JUF7.
Proteomic databases
PRIDE	B0JUF7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5865413.
GenomeReviews	Gene locus MAE_45830 in contig AP009552_GR.
KEGG	mar:MAE_45830.
PATRIC	22633701. VBIMicAer59304_4161.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG672839.
OMA	SKVIVVC.
PhylomeDB	B0JUF7.
ProtClustDB	PRK00155.
Enzyme and pathway databases
BioCyc	MAER449447:MAE_45830-MONOMER.
Family and domain databases
HAMAP	MF_00108. IspD. [Tree]
InterPro	IPR001228. ISPD_synthase. IPR018294. ISPD_synthase_CS. [Graphical view]
KO	K00991.
Pfam	PF01128. IspD. 1 hit. [Graphical view]
TIGRFAMs	TIGR00453. IspD. 1 hit.
PROSITE	PS01295. ISPD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ISPD_MICAN

Accession

Primary (citable) accession number: B0JUF7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	March 18, 2008
Last modified:	January 25, 2012
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program