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B0JSP4

- SYI_MICAN

UniProt

B0JSP4 - SYI_MICAN

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Microcystis aeruginosa (strain NIES-843)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 46 (01 Oct 2014)
      Sequence version 1 (18 Mar 2008)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei562 – 5621Aminoacyl-adenylateUniRule annotation
    Binding sitei606 – 6061ATPUniRule annotation
    Metal bindingi921 – 9211ZincUniRule annotation
    Metal bindingi924 – 9241ZincUniRule annotation
    Metal bindingi941 – 9411ZincUniRule annotation
    Metal bindingi944 – 9441ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciMAER449447:GHO8-1118-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:MAE_11160
    OrganismiMicrocystis aeruginosa (strain NIES-843)
    Taxonomic identifieri449447 [NCBI]
    Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesMicrocystis
    ProteomesiUP000001510: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 952952Isoleucine--tRNA ligasePRO_1000088549Add
    BLAST

    Proteomic databases

    PaxDbiB0JSP4.
    PRIDEiB0JSP4.

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi449447.MAE_11160.

    Structurei

    3D structure databases

    ProteinModelPortaliB0JSP4.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi60 – 7011"HIGH" regionAdd
    BLAST
    Motifi603 – 6075"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiKPVHWCL.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B0JSP4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSNAKSYKDT VNLPQTDFDM RANASKREPE IQKFWQDEQI YEKLAQNNPK    50
    ELFILHDGPP YANGSLHIGH ALNKILKDII NKYKLLQGYK VRYVPGWDCH 100
    GLPIELKVLQ SLKSSEKEGL TPVKLRQKAH DFALQTQKEQ CEGFKRYGVW 150
    GDWENPYLTL QPEYEAAQIA VFGKMALKGY IYRGLKPVHW SPSSQTALAE 200
    AELEYPEGHT SRSVYVAFPI TSVSTPVLRP FLPNLSVAIW TTTPWTLPGN 250
    LAVALNPELN YSVVETSESN YLIVATDLVE KLADTFNRTL TIKATVKGLE 300
    LEHTKYRHPL FDRESAILIG GDYVTTDSGT GLVHTAPGHG QEDYIVGQRY 350
    GLPILSPVDD KGNFTAEAGQ FAGLNVLKDA NEAIILALTE KGALLKEEAY 400
    QHKYPYDWRT KKPTIFRATE QWFASVEGFR ELALEAIDSV RWIPATGKNR 450
    ITSMVSERSD WCISRQRSWG VPIPVFYDEE TNEPLLTEET INHVQAIFGV 500
    KGSNAWWELS VEELLPPSYR NNGRSYRKGM DTMDVWFDSG SSWNAVANAR 550
    PELSYPADMY LEGSDQHRGW FQSSLLTSVA ANGIAPYKTV LTHGFVLDEQ 600
    GRKMSKSLGN VIDPNVIING GKDQKKEPAY GVDVIRLWVS SVDYTNDVNI 650
    GQNILKQLVD IRNKIRNTAR FLLGSLNDFD PVKDAVAYED LPEIDRYMLH 700
    RISEVFTEVT AAFESFQFFR FFQTVQNFCV VDLSNFYIDI AKDRLYISDP 750
    NSFRRRSCQT VYAIALENLA KAIAPVLSHL AEDIWQFLPY KTPYLSVFES 800
    GWLNIDPAWN NRELADKWAK FRQLRTEVNK VMETARNDKA IGASLEAKVL 850
    LYVPDESLQQ ELELFNNCDS LTGNKVDELR YLFLSSQVEL VSDISAIQTA 900
    EYKGESDFVS VGIVKAEGEK CDRCWNYSTQ VGKFADDPTI CERCNAALKG 950
    EF 952
    Length:952
    Mass (Da):107,865
    Last modified:March 18, 2008 - v1
    Checksum:iEF727BAFF613E084
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP009552 Genomic DNA. Translation: BAG00938.1.
    RefSeqiYP_001656130.1. NC_010296.1.

    Genome annotation databases

    EnsemblBacteriaiBAG00938; BAG00938; MAE_11160.
    GeneIDi5861914.
    KEGGimar:MAE_11160.
    PATRICi22627365. VBIMicAer59304_1022.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP009552 Genomic DNA. Translation: BAG00938.1 .
    RefSeqi YP_001656130.1. NC_010296.1.

    3D structure databases

    ProteinModelPortali B0JSP4.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 449447.MAE_11160.

    Proteomic databases

    PaxDbi B0JSP4.
    PRIDEi B0JSP4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAG00938 ; BAG00938 ; MAE_11160 .
    GeneIDi 5861914.
    KEGGi mar:MAE_11160.
    PATRICi 22627365. VBIMicAer59304_1022.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi KPVHWCL.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci MAER449447:GHO8-1118-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: NIES-843.

    Entry informationi

    Entry nameiSYI_MICAN
    AccessioniPrimary (citable) accession number: B0JSP4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 20, 2008
    Last sequence update: March 18, 2008
    Last modified: October 1, 2014
    This is version 46 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3