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B0JSP4

- SYI_MICAN

UniProt

B0JSP4 - SYI_MICAN

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Microcystis aeruginosa (strain NIES-843)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei562 – 5621Aminoacyl-adenylateUniRule annotation
Binding sitei606 – 6061ATPUniRule annotation
Metal bindingi921 – 9211ZincUniRule annotation
Metal bindingi924 – 9241ZincUniRule annotation
Metal bindingi941 – 9411ZincUniRule annotation
Metal bindingi944 – 9441ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciMAER449447:GHO8-1118-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:MAE_11160
OrganismiMicrocystis aeruginosa (strain NIES-843)
Taxonomic identifieri449447 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesMicrocystis
ProteomesiUP000001510: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 952952Isoleucine--tRNA ligasePRO_1000088549Add
BLAST

Proteomic databases

PaxDbiB0JSP4.
PRIDEiB0JSP4.

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi449447.MAE_11160.

Structurei

3D structure databases

ProteinModelPortaliB0JSP4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi60 – 7011"HIGH" regionAdd
BLAST
Motifi603 – 6075"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B0JSP4-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSNAKSYKDT VNLPQTDFDM RANASKREPE IQKFWQDEQI YEKLAQNNPK
60 70 80 90 100
ELFILHDGPP YANGSLHIGH ALNKILKDII NKYKLLQGYK VRYVPGWDCH
110 120 130 140 150
GLPIELKVLQ SLKSSEKEGL TPVKLRQKAH DFALQTQKEQ CEGFKRYGVW
160 170 180 190 200
GDWENPYLTL QPEYEAAQIA VFGKMALKGY IYRGLKPVHW SPSSQTALAE
210 220 230 240 250
AELEYPEGHT SRSVYVAFPI TSVSTPVLRP FLPNLSVAIW TTTPWTLPGN
260 270 280 290 300
LAVALNPELN YSVVETSESN YLIVATDLVE KLADTFNRTL TIKATVKGLE
310 320 330 340 350
LEHTKYRHPL FDRESAILIG GDYVTTDSGT GLVHTAPGHG QEDYIVGQRY
360 370 380 390 400
GLPILSPVDD KGNFTAEAGQ FAGLNVLKDA NEAIILALTE KGALLKEEAY
410 420 430 440 450
QHKYPYDWRT KKPTIFRATE QWFASVEGFR ELALEAIDSV RWIPATGKNR
460 470 480 490 500
ITSMVSERSD WCISRQRSWG VPIPVFYDEE TNEPLLTEET INHVQAIFGV
510 520 530 540 550
KGSNAWWELS VEELLPPSYR NNGRSYRKGM DTMDVWFDSG SSWNAVANAR
560 570 580 590 600
PELSYPADMY LEGSDQHRGW FQSSLLTSVA ANGIAPYKTV LTHGFVLDEQ
610 620 630 640 650
GRKMSKSLGN VIDPNVIING GKDQKKEPAY GVDVIRLWVS SVDYTNDVNI
660 670 680 690 700
GQNILKQLVD IRNKIRNTAR FLLGSLNDFD PVKDAVAYED LPEIDRYMLH
710 720 730 740 750
RISEVFTEVT AAFESFQFFR FFQTVQNFCV VDLSNFYIDI AKDRLYISDP
760 770 780 790 800
NSFRRRSCQT VYAIALENLA KAIAPVLSHL AEDIWQFLPY KTPYLSVFES
810 820 830 840 850
GWLNIDPAWN NRELADKWAK FRQLRTEVNK VMETARNDKA IGASLEAKVL
860 870 880 890 900
LYVPDESLQQ ELELFNNCDS LTGNKVDELR YLFLSSQVEL VSDISAIQTA
910 920 930 940 950
EYKGESDFVS VGIVKAEGEK CDRCWNYSTQ VGKFADDPTI CERCNAALKG

EF
Length:952
Mass (Da):107,865
Last modified:March 18, 2008 - v1
Checksum:iEF727BAFF613E084
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP009552 Genomic DNA. Translation: BAG00938.1.
RefSeqiYP_001656130.1. NC_010296.1.

Genome annotation databases

EnsemblBacteriaiBAG00938; BAG00938; MAE_11160.
GeneIDi5861914.
KEGGimar:MAE_11160.
PATRICi22627365. VBIMicAer59304_1022.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP009552 Genomic DNA. Translation: BAG00938.1 .
RefSeqi YP_001656130.1. NC_010296.1.

3D structure databases

ProteinModelPortali B0JSP4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 449447.MAE_11160.

Proteomic databases

PaxDbi B0JSP4.
PRIDEi B0JSP4.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAG00938 ; BAG00938 ; MAE_11160 .
GeneIDi 5861914.
KEGGi mar:MAE_11160.
PATRICi 22627365. VBIMicAer59304_1022.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci MAER449447:GHO8-1118-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NIES-843.

Entry informationi

Entry nameiSYI_MICAN
AccessioniPrimary (citable) accession number: B0JSP4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: March 18, 2008
Last modified: October 29, 2014
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3