B0JSD7 (SYGA_MICAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycine--tRNA ligase alpha subunit
EC=6.1.1.14

Alternative name(s):
Glycyl-tRNA synthetase alpha subunit
Short name=GlyRS

Gene names

Name:	glyQ
Ordered Locus Names:	MAE_42730

Organism

Microcystis aeruginosa (strain NIES-843) [Complete proteome] [HAMAP]

Taxonomic identifier

449447 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Microcystis

Protein attributes

Sequence length	291 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-tRNA(Gly). HAMAP MF_00254
Subunit structure	Tetramer of two alpha and two beta subunits By similarity.
Subcellular location	Cytoplasm By similarity HAMAP MF_00254.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glycine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 291	291	Glycine--tRNA ligase alpha subunit HAMAP MF_00254		PRO_1000078528

Sequences

	Sequence		Length	Mass (Da)
	B0JSD7 [UniParc]. Last modified March 18, 2008. Version 1. Checksum: 227A2E2B6659D272	FASTA	291	33,608
	10 20 30 40 50 60 MSLTFQAVIA KLNEFWADRG CLVAQPYDTE KGAGTMSPHT FLRAIGPEPW AVAYVEPCRR 70 80 90 100 110 120 PTDGRYGENP NRFQHYYQYQ VLIKPSPDNI QEIYLDSLRV LGINPEDHDI RFVEDNWESP 130 140 150 160 170 180 TLGAWGVGWE VWLDGMEITQ FTYFQQCGGI DCRPVAIEIT YGLERLAMYL QDVEAINKIQ 190 200 210 220 230 240 WNENILYGDI FLQNEIEQCT YNFEASNPEL LFSLFSLYEQ EAKQLIDRSL VIPSLDYVLK 250 260 270 280 290 CSHTFNLLDA RGVIAVAERT RYIGRIRNLA RQVAQLYLQQ REALGFPLQT V

References

[1]

"Complete genomic structure of the bloom-forming toxic cyanobacterium Microcystis aeruginosa NIES-843." Kaneko T., Nakajima N., Okamoto S., Suzuki I., Tanabe Y., Tamaoki M., Nakamura Y., Kasai F., Watanabe A., Kawashima K., Kishida Y., Ono A., Shimizu Y., Takahashi C., Minami C., Fujishiro T., Kohara M., Katoh M. , Nakazaki N., Nakayama S., Yamada M., Tabata S., Watanabe M.M. DNA Res. 14:247-256(2007) [PubMed: 18192279] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NIES-843.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP009552 Genomic DNA. Translation: BAG04095.1.
RefSeq	YP_001659287.1. NC_010296.1.
3D structure databases
ProteinModelPortal	B0JSD7.
SMR	B0JSD7. Positions 3-279.
ModBase	Search...
Protein-protein interaction databases
STRING	B0JSD7.
Proteomic databases
PRIDE	B0JSD7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5865103.
GenomeReviews	Gene locus MAE_42730 in contig AP009552_GR.
KEGG	mar:MAE_42730.
PATRIC	22633141. VBIMicAer59304_3881.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG285316.
OMA	CRRPTDG.
PhylomeDB	B0JSD7.
ProtClustDB	PRK09348.
Enzyme and pathway databases
BioCyc	MAER449447:MAE_42730-MONOMER.
Family and domain databases
HAMAP	MF_00254. Gly_tRNA_synth_alpha. [Tree]
InterPro	IPR006194. Gly-tRNA-synth_II_heterodimer. IPR002310. Gly-tRNA_synth_IIc_asu. [Graphical view]
KO	K01878.
Pfam	PF02091. tRNA-synt_2e. 1 hit. [Graphical view]
PRINTS	PR01044. TRNASYNTHGA.
TIGRFAMs	TIGR00388. GlyQ. 1 hit.
PROSITE	PS50861. AA_TRNA_LIGASE_II_GLYAB. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYGA_MICAN

Accession

Primary (citable) accession number: B0JSD7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	March 18, 2008
Last modified:	January 25, 2012
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program