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B0JQ90 (B0JQ90_MICAN) Unreviewed, UniProtKB/TrEMBL

Last modified May 1, 2013. Version 37. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Glutathione synthetase HAMAP-Rule MF_00162
EC=6.3.2.3 HAMAP-Rule MF_00162
Alternative name(s):
GSH synthetase HAMAP-Rule MF_00162
Glutathione synthase HAMAP-Rule MF_00162
Gene names
Name:gshB HAMAP-Rule MF_00162 EMBL BAG00549.1
Ordered Locus Names:MAE_07270
OrganismMicrocystis aeruginosa (strain NIES-843) [Complete proteome] [HAMAP]
Taxonomic identifier449447 [NCBI]
Taxonomic lineageBacteria › Cyanobacteria › Oscillatoriophycideae › Chroococcales › Microcystis ›

Protein attributes

Sequence length326 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione. HAMAP-Rule MF_00162

Cofactor

Binds (magnesium or manganese,ion) per subunit By similarity. HAMAP-Rule MF_00162

Pathway

Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 2/2. HAMAP-Rule MF_00162

Sequence similarities

Belongs to the prokaryotic GSH synthase family. HAMAP-Rule MF_00162

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00162

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain132 – 317186ATP-grasp By similarity HAMAP-Rule MF_00162
Nucleotide binding159 – 21557ATP By similarity HAMAP-Rule MF_00162

Sites

Metal binding2881Magnesium or manganese By similarity HAMAP-Rule MF_00162
Metal binding2901Magnesium or manganese By similarity HAMAP-Rule MF_00162

Sequences

Sequence LengthMass (Da)Tools
B0JQ90 [UniParc].

Last modified March 18, 2008. Version 1.
Checksum: 775953D543BE6568

FASTA32636,309
        10         20         30         40         50         60 
MKLAFIIDPI EYLDPGHDTS VAIMEASQLL GHEVWMTSIT DLSAIAGKAW AKLTKIELVP 

        70         80         90        100        110        120 
VELKDNHWEA VSQWYQRQET VFTCLENFDF VWMRKDPPVT IAYLYATYLL DLIDPQKTQV 

       130        140        150        160        170        180 
INSTRGLRHA NEKLYTIHFA QVMPATIVSQ DKATIREFVN QKGAAVMKPL GGKAGEGILF 

       190        200        210        220        230        240 
LSPEDRNLNS MIEISTKWGQ EPVMIQDYLP AAKEGDKRII VLNGEPIGAV NRIPTGSEFR 

       250        260        270        280        290        300 
GNMAVGGRVA KTEISDQELE ICATLAPKLR EDGLYFVGLD VIGGYLTEVN VTSPTGIREI 

       310        320 
DRLNNVSLGQ QVIKWLENFA CAKALS

« Hide

References

[1]"Complete genomic structure of the bloom-forming toxic cyanobacterium Microcystis aeruginosa NIES-843."
Kaneko T., Nakajima N., Okamoto S., Suzuki I., Tanabe Y., Tamaoki M., Nakamura Y., Kasai F., Watanabe A., Kawashima K., Kishida Y., Ono A., Shimizu Y., Takahashi C., Minami C., Fujishiro T., Kohara M., Katoh M. expand/collapse author list , Nakazaki N., Nakayama S., Yamada M., Tabata S., Watanabe M.M.
DNA Res. 14:247-256(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: NIES-843.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AP009552 Genomic DNA. Translation: BAG00549.1.
RefSeqYP_001655741.1. NC_010296.1.

3D structure databases

ProteinModelPortalB0JQ90.
ModBaseSearch...

Protein-protein interaction databases

STRING449447.MAE_07270.
B0JQ90.

Proteomic databases

PaxDbB0JQ90.
PRIDEB0JQ90.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAG00549; BAG00549; MAE_07270.
GeneID5861524.
KEGGmar:MAE_07270.
PATRIC22626657. VBIMicAer59304_0669.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0189.
HOGENOMHOG000265022.
KOK01920.
OMAWMRKDPP.
ProtClustDBPRK05246.

Enzyme and pathway databases

BioCycMAER449447:GHO8-728-MONOMER.
UniPathwayUPA00142; UER00210.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPMF_00162. GSH_S.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR006284. Glut_synth_pro.
IPR004218. GSHS_ATP-bd.
IPR004215. GSHS_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PfamPF02955. GSH-S_ATP. 1 hit.
PF02951. GSH-S_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. PreATP-grasp-like. 1 hit.
TIGRFAMsTIGR01380. glut_syn. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameB0JQ90_MICAN
AccessionPrimary (citable) accession number: B0JQ90
Entry history
Integrated into UniProtKB/TrEMBL: March 18, 2008
Last sequence update: March 18, 2008
Last modified: May 1, 2013
This is version 37 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info