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B0JNA3 (B0JNA3_MICAN) Unreviewed, UniProtKB/TrEMBL

Last modified May 1, 2013. Version 36. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
DNA gyrase subunit A HAMAP-Rule MF_01897
EC=5.99.1.3 HAMAP-Rule MF_01897
Gene names
Name:gyrA HAMAP-Rule MF_01897 EMBL BAG00267.1
Ordered Locus Names:MAE_04450
OrganismMicrocystis aeruginosa (strain NIES-843) [Complete proteome] [HAMAP]
Taxonomic identifier449447 [NCBI]
Taxonomic lineageBacteria › Cyanobacteria › Oscillatoriophycideae › Chroococcales › Microcystis ›

Protein attributes

Sequence length850 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings By similarity. HAMAP-Rule MF_01897

Catalytic activity

ATP-dependent breakage, passage and rejoining of double-stranded DNA. HAMAP-Rule MF_01897 SAAS SAAS005743

Subunit structure

Heterotetramer, composed of two GyrA and two GyrB chains. Within the heterotetramer, GyrA contains the active site tyrosine that forms a covalent intermediate with the DNA, while GyrB contributes the cofactor binding sites and catalyzes ATP hydrolysis By similarity. HAMAP-Rule MF_01897

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_01897.

Sequence similarities

Belongs to the topoisomerase GyrA/ParC subunit family. HAMAP-Rule MF_01897

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Active site1221O-(5'-phospho-DNA)-tyrosine intermediate By similarity HAMAP-Rule MF_01897

Sequences

Sequence LengthMass (Da)Tools
B0JNA3 [UniParc].

Last modified March 18, 2008. Version 1.
Checksum: 9C155EBD19C67B80

FASTA85094,325
        10         20         30         40         50         60 
MTAAQDHVVP TDLSNEMSRS YLEYAMSVIV GRALPDARDG LKPVHRRILY AMYELGLTPD 

        70         80         90        100        110        120 
RPFRKCARVV GEVLGKYHPH GDTAVYDALV RMAQDFSMRD PLINGHGNFG SIDNDPPAAM 

       130        140        150        160        170        180 
RYTECRLHTL ATNALLQDIE SETVDFADNF DGSQQEPVVL PSRVPQLLIN GSSGIAVGMA 

       190        200        210        220        230        240 
TNIPPHNLAE IIDGTVALIH NPDLTDTELQ RYIPGPDFPT GGHILGRDGI QEAYTTGRGS 

       250        260        270        280        290        300 
ITLRGVATIE TIEQRGRPDR DAIIITELPY QTNKAALIEK IADLVNDRKI DGISDVRDES 

       310        320        330        340        350        360 
DRDGIRVVIE LKRDAYPRVV LNNLYKQTPL QSNFGANMLA LVDNEPRLLT LREFLRVFLD 

       370        380        390        400        410        420 
FRVEVITRRT RYELRKAEER DHILQGLLIA LASLDAVIAL IRSAADTASA KEGLVQNFGL 

       430        440        450        460        470        480 
SEVQADAILQ MQLRRLTALE SEKIQQEHGE LLAKIGDLQD ILAKKERIDS IIEEELQQIK 

       490        500        510        520        530        540 
TIHATPRRTH IEQREGEIAE TDLIANEQAL ILVTEQGYIK RMPVNTFESQ NRATRGKAGA 

       550        560        570        580        590        600 
KMKEDDGIDH FITCRDHDSV LFFTDRGVVY SLNAYHIPTG SRTARGVPIV QLLEIPKGEK 

       610        620        630        640        650        660 
ITSVVAVSEF SEDNYLIMLT QKGFIKKTAL AAFSNIRSNG LIAISLEDGD QLRWVRLAKE 

       670        680        690        700        710        720 
EDSVIIGSRQ GMAIHFKADS QQLRSLGRAT KGVKSMKLRS GDELISMDIL PSQIVAQIAE 

       730        740        750        760        770        780 
SNEEETEDES EEINPEVANN GPWVLAITMA GLGKRVPVTL LRLQNRAGLG VRVIKFRKKN 

       790        800        810        820        830        840 
DKLAALHVVN PHEEFMIITE RGIIIRQSVD AITPQSRSAG GIRVQKLDED DAIAAVALVP 

       850 
PSDAEESEDI

« Hide

References

[1]"Complete genomic structure of the bloom-forming toxic cyanobacterium Microcystis aeruginosa NIES-843."
Kaneko T., Nakajima N., Okamoto S., Suzuki I., Tanabe Y., Tamaoki M., Nakamura Y., Kasai F., Watanabe A., Kawashima K., Kishida Y., Ono A., Shimizu Y., Takahashi C., Minami C., Fujishiro T., Kohara M., Katoh M. expand/collapse author list , Nakazaki N., Nakayama S., Yamada M., Tabata S., Watanabe M.M.
DNA Res. 14:247-256(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: NIES-843.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AP009552 Genomic DNA. Translation: BAG00267.1.
RefSeqYP_001655459.1. NC_010296.1.

3D structure databases

ProteinModelPortalB0JNA3.
SMRB0JNA3. Positions 30-491.
ModBaseSearch...

Protein-protein interaction databases

STRING449447.MAE_04450.
B0JNA3.

Proteomic databases

PaxDbB0JNA3.
PRIDEB0JNA3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAG00267; BAG00267; MAE_04450.
GeneID5861081.
KEGGmar:MAE_04450.
PATRIC22626161. VBIMicAer59304_0421.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0188.
HOGENOMHOG000076278.
KOK02469.
OMAIAQEDVV.
ProtClustDBPRK05560.

Enzyme and pathway databases

BioCycMAER449447:GHO8-445-MONOMER.

Family and domain databases

Gene3D3.30.1360.40. 1 hit.
3.90.199.10. 2 hits.
HAMAPMF_01897. GyrA.
InterProIPR024946. Arg_repress_C-like.
IPR005743. GyrA.
IPR006691. GyrA/parC_pinwhl.
IPR002205. Topo_IIA_A/C.
IPR013758. Topo_IIA_A/C_ab.
IPR013760. Topo_IIA_like_dom.
[Graphical view]
PfamPF03989. DNA_gyraseA_C. 6 hits.
PF00521. DNA_topoisoIV. 1 hit.
[Graphical view]
SMARTSM00434. TOP4c. 1 hit.
[Graphical view]
SUPFAMSSF56719. Topo_IIA_cen. 1 hit.
TIGRFAMsTIGR01063. gyrA. 1 hit.
ProtoNetSearch...

Entry information

Entry nameB0JNA3_MICAN
AccessionPrimary (citable) accession number: B0JNA3
Entry history
Integrated into UniProtKB/TrEMBL: March 18, 2008
Last sequence update: March 18, 2008
Last modified: May 1, 2013
This is version 36 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info